Library
local_library Home Repository

In Vivo Prevention of Implant-Associated Infections Caused by Antibiotic-Resistant Bacteria through Biofunctionalization of Additively Manufactured Porous Titanium

Journal Article (2023)
Author(s)

I.A.J. Van Hengel (TU Delft - Biomaterials & Tissue Biomechanics)

B. van Dijk (University Medical Center Utrecht)

K. Modaresifar (TU Delft - Biomaterials & Tissue Biomechanics)

J. Fred F. Hooning van Duyvenbode (University Medical Center Utrecht)

F. Ruben H.A. Nurmohamed (University Medical Center Utrecht)

M.A. Leeflang (TU Delft - Biomaterials & Tissue Biomechanics)

A.C. Fluit (University Medical Center Utrecht)

E.L. Fratila-Apachitei (TU Delft - Biomaterials & Tissue Biomechanics)

I. Apachitei (TU Delft - Biomaterials & Tissue Biomechanics)

H. Weinans (TU Delft - Biomaterials & Tissue Biomechanics, University Medical Center Utrecht)

A.A. Zadpoor (TU Delft - Biomaterials & Tissue Biomechanics)

Research Group
Biomaterials & Tissue Biomechanics
Copyright
© 2023 I.A.J. van Hengel, B. van Dijk, K. Modaresifar, J. Fred F. Hooning van Duyvenbode, F. Ruben H.A. Nurmohamed, M.A. Leeflang, A.C. Fluit, E.L. Fratila-Apachitei, I. Apachitei, Harrie Weinans, A.A. Zadpoor
DOI related publication
https://doi.org/10.3390/jfb14100520
More Info
expand_more
Publication Year
2023
Language
English
Copyright
© 2023 I.A.J. van Hengel, B. van Dijk, K. Modaresifar, J. Fred F. Hooning van Duyvenbode, F. Ruben H.A. Nurmohamed, M.A. Leeflang, A.C. Fluit, E.L. Fratila-Apachitei, I. Apachitei, Harrie Weinans, A.A. Zadpoor
Research Group
Biomaterials & Tissue Biomechanics
Issue number
10
Volume number
14
Reuse Rights

Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.

Abstract

Additively manufactured (AM) porous titanium implants may have an increased risk of implant-associated infection (IAI) due to their huge internal surfaces. However, the same surface, when biofunctionalized, can be used to prevent IAI. Here, we used a rat implant infection model to evaluate the biocompatibility and infection prevention performance of AM porous titanium against bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). The specimens were biofunctionalized with Ag nanoparticles (NPs) using plasma electrolytic oxidation (PEO). Infection was initiated using either intramedullary injection in vivo or with in vitro inoculation of the implant prior to implantation. Nontreated (NT) implants were compared with PEO-treated implants with Ag NPs (PT-Ag), without Ag NPs (PT) and infection without an implant. After 7 days, the bacterial load and bone morphological changes were evaluated. When infection was initiated through in vivo injection, the presence of the implant did not enhance the infection, indicating that this technique may not assess the prevention but rather the treatment of IAIs. Following in vitro inoculation, the bacterial load on the implant and in the peri-implant bony tissue was reduced by over 90% for the PT-Ag implants compared to the PT and NT implants. All infected groups had enhanced osteomyelitis scores compared to the noninfected controls.