3D particle averaging and detection of macromolecular symmetry in localization microscopy

Journal article (2021)

Authors

H. Heydarian ImPhys/Computational Imaging - AS
M.J. Joosten BN/Arjen Jakobi Lab - AS
A. Przybylski Max Planck Insitute for Biophysical Chemistry, Göttingen
Florian Schueder Max Planck Institute of Biochemistry, Ludwig Maximilians University
Ralf Jungmann Ludwig Maximilians University, Max Planck Institute of Biochemistry
B.J.C. van Werkhoven ImPhys/Computational Imaging - AS
J. Keller-Fiendeisen Max Planck Insitute for Biophysical Chemistry, Göttingen
B. Rieger ImPhys/Computational Imaging - AS
S. Stallinga ImPhys/Imaging Physics
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Research Group
ImPhys/Computational Imaging (AS) (TU Delft)
Copyright: © 2021 H. Heydarian, M.J. Joosten, A. Przybylski, Florian Schueder, Ralf Jungmann, B.J.C. van Werkhoven, J. Keller-Fiendeisen, B. Rieger, S. Stallinga, More Authors
DOI
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https://doi.org/10.1038/s41467-021-22006-5
OA-Fund TU Delft
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Published Date

2021

Language

English

Faculty

Applied Sciences

Department

ImPhys/Imaging Physics

Research Group

ImPhys/Computational Imaging

Abstract

Single molecule localization microscopy offers in principle resolution down to the molecular level, but in practice this is limited primarily by incomplete fluorescent labeling of the structure. This missing information can be completed by merging information from many structurally identical particles. In this work, we present an approach for 3D single particle analysis in localization microscopy which hugely increases signal-to-noise ratio and resolution and enables determining the symmetry groups of macromolecular complexes. Our method does not require a structural template, and handles anisotropic localization uncertainties. We demonstrate 3D reconstructions of DNA-origami tetrahedrons, Nup96 and Nup107 subcomplexes of the nuclear pore complex acquired using multiple single molecule localization microscopy techniques, with their structural symmetry deducted from the data.