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3D particle averaging and detection of macromolecular symmetry in localization microscopy

Journal Article (2021)
Authors

H. Heydarian (ImPhys/Computational Imaging)

M.J. Joosten (TU Delft - BN/Arjen Jakobi Lab)

A. Przybylski (Max Planck Insitute for Biophysical Chemistry, Göttingen)

Florian Schueder (Max Planck Institute of Biochemistry, Ludwig Maximilians University)

Ralf Jungmann (Ludwig Maximilians University, Max Planck Institute of Biochemistry)

B.J.C. van Werkhoven (ImPhys/Computational Imaging)

J. Keller-Fiendeisen (Max Planck Insitute for Biophysical Chemistry, Göttingen)

B. Rieger (ImPhys/Computational Imaging)

S Stallinga (TU Delft - ImPhys/Imaging Physics)

G.B. More authors (External organisation)

Research Group
ImPhys/Computational Imaging
Copyright
© 2021 H. Heydarian, M.J. Joosten, A. Przybylski, Florian Schueder, Ralf Jungmann, B.J.C. van Werkhoven, J. Keller-Fiendeisen, B. Rieger, S. Stallinga, More Authors
To reference this document use:
https://doi.org/10.1038/s41467-021-22006-5
More Info
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Publication Year
2021
Language
English
Copyright
© 2021 H. Heydarian, M.J. Joosten, A. Przybylski, Florian Schueder, Ralf Jungmann, B.J.C. van Werkhoven, J. Keller-Fiendeisen, B. Rieger, S. Stallinga, More Authors
Related content
Research Group
ImPhys/Computational Imaging
Issue number
1
Volume number
12
DOI:
https://doi.org/10.1038/s41467-021-22006-5
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Abstract

Single molecule localization microscopy offers in principle resolution down to the molecular level, but in practice this is limited primarily by incomplete fluorescent labeling of the structure. This missing information can be completed by merging information from many structurally identical particles. In this work, we present an approach for 3D single particle analysis in localization microscopy which hugely increases signal-to-noise ratio and resolution and enables determining the symmetry groups of macromolecular complexes. Our method does not require a structural template, and handles anisotropic localization uncertainties. We demonstrate 3D reconstructions of DNA-origami tetrahedrons, Nup96 and Nup107 subcomplexes of the nuclear pore complex acquired using multiple single molecule localization microscopy techniques, with their structural symmetry deducted from the data.