Print Email Facebook Twitter Continuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11 Title Continuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11 Author Gelain, L. (TU Delft OLD BT/Cell Systems Engineering; University of Campinas) Kingma, Esther (Student TU Delft) Geraldo da Cruz Pradella, José (Universidade Federal de Sao Paulo) Carvalho da Costa, Aline (University of Campinas) van der Wielen, L.A.M. (TU Delft BT/Bioprocess Engineering; University of Limerick) van Gulik, W.M. (TU Delft BT/Industriele Microbiologie) Date 2020 Abstract Carbon-limited chemostat cultures were performed using different carbon sources (glucose, 10 and 20 g/L; sucrose, 10 g/L; fructose/glucose, 5.26/5.26 g/L; carboxymethyl cellulose, 10 g/L; and carboxymethyl cellulose/glucose, 5/5 g/L) to verify the capability of the wild type strain Trichoderma harzianum to produce extracellular enzymes. All chemostat cultures were carried out at a fixed dilution rate of 0.05 h−1. Experiments using glucose, fructose/glucose and sucrose were performed in duplicate. Glucose condition was found to induce the production of enzymes that can catalyse the hydrolysis of p-nitrophenyl-β-D-glucopyranoside (PNPGase). A concentration of 20 g/L of glucose in the feed provided the highest productivity (1048 ± 16 U/mol h). Extracellular polysaccharides were considered the source of inducers. Based on the obtained results, a new PNPGase production process was developed using mainly glucose. This process raises interesting possibilities of synthesizing the inducer substrate and the induced enzymes in a single step using an easily assimilated carbon source under carbon-limited conditions. Subject Carbon limitationContinuous fermentation processEnzyme productionExtracellular polysaccharidesGlucosePNPGase To reference this document use: http://resolver.tudelft.nl/uuid:4145d5da-1a3d-4d25-a519-510f91c6e0d5 DOI https://doi.org/10.1016/j.funbio.2020.10.008 ISSN 1878-6146 Source Fungal Biology, 125 (3), 177-183 Part of collection Institutional Repository Document type journal article Rights © 2020 L. Gelain, Esther Kingma, José Geraldo da Cruz Pradella, Aline Carvalho da Costa, L.A.M. van der Wielen, W.M. van Gulik Files PDF 1_s2.0_S1878614620301604_main.pdf 953.39 KB Close viewer /islandora/object/uuid:4145d5da-1a3d-4d25-a519-510f91c6e0d5/datastream/OBJ/view