Print Email Facebook Twitter Bacterial diversity and activity along a salinity gradient in soda lakes of the Kulunda Steppe (Altai, Russia) Title Bacterial diversity and activity along a salinity gradient in soda lakes of the Kulunda Steppe (Altai, Russia) Author Foti, M.J. Sorokin, D.Y. Zacharova, E.E. Pimenov, N.V. Kuenen, J.G. Muyzer, G. Faculty Applied Sciences Department Biotechnology Date 2007-11-08 Abstract Here we describe the diversity and activity of sulfate reducing bacteria along a salinity gradient in four different soda lakes from the Kulunda Steppe (South East Siberia, Russia). For this purpose, a combination of culture-dependent and independent techniques was applied. The general bacterial and SRB diversity were analyzed by denaturing gradient gel electrophoresis (DGGE) targeting the 16S rDNA gene. DNA was used to detect the microbial populations that were present in the soda lake sediments, whereas ribosomal RNA was used as a template to obtain information on those that were active. Individual DGGE bands were sequenced and a phylogenetic analysis was performed. In addition, the overall activity of SRB was obtained by measuring the sulfate reduction rates (SRR) and their abundance was estimated by serial dilution. Our results showed the presence of minor, but highly active microbial populations, mostly represented by members of the Proteobacteria. Remarkably high SRR were measured at hypersaline conditions (200 g L-1). A relatively high viable count indicated that sulfate reducing bacteria could be highly active in hypersaline soda lakes. Furthermore, the increase of sodium carbonate/bicarbonate seemed to affect the composition of the microbial community in soda lakes, but not the rate of sulfate reduction. To reference this document use: http://resolver.tudelft.nl/uuid:50f77f09-958a-431c-84fd-f2b1aa3bd7fd DOI https://doi.org/10.1007/s00792-007-0117-7 Publisher Springer ISSN 1431-0651 Source Extremophiles, 12 (1), 2008 Part of collection Institutional Repository Document type journal article Rights (c) 2007 Foti, M.J. et al. ; Springer Files PDF Foti_2007.pdf 500.49 KB Close viewer /islandora/object/uuid:50f77f09-958a-431c-84fd-f2b1aa3bd7fd/datastream/OBJ/view