P450BM3-Catalyzed oxidations employing dual functional small molecules

Title

P450BM3-Catalyzed oxidations employing dual functional small molecules

 Author

Willot, S.J. (TU Delft BT/Biocatalysis)
Tieves, F. (TU Delft BT/Biocatalysis)
Girhard, Marco (Universität Düsseldorf)
Urlacher, Vlada B. (Universität Düsseldorf)
Hollmann, F. (TU Delft BT/Biocatalysis) ORCID 0000-0003-4821-756X
De Gonzalo, G. (University of Seville)

Date

2019

 Abstract

A set of dual functional small molecules (DFSMs) containing different amino acids has been synthesized and employed together with three different variants of the cytochrome P450 monooxygenase P450BM3 from Bacillus megaterium in H2O2-dependent oxidation reactions. These DFSMs enhance P450BM3 activity with hydrogen peroxide as an oxidant, converting these enzymes into formal peroxygenases. This system has been employed for the catalytic epoxidation of styrene and in the sulfoxidation of thioanisole. Various P450BM3 variants have been evaluated in terms of activity and selectivity of the peroxygenase reactions.


 Subject

Biocatalysis
Cytochrome P450
Dual functional small molecules
Epoxidations
Sulfoxidation

 To reference this document use:

http://resolver.tudelft.nl/uuid:53df8061-0bdd-4e24-97e9-ebe23f2a47f1

 DOI

https://doi.org/10.3390/catal9070567

 ISSN

2073-4344

 Source

Catalysts, 9 (7)

 Part of collection

Institutional Repository

 Document type

journal article

 Rights

© 2019 S.J. Willot, F. Tieves, Marco Girhard, Vlada B. Urlacher, F. Hollmann, G. De Gonzalo
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