Print Email Facebook Twitter Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures Title Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures Author Rombouts, J.L. (TU Delft BT/Environmental Biotechnology) Kranendonk, Elsemiek Madeleine Maria (Student TU Delft) Regueira, Alberte (Universidade de Santiago de Compostela) Weissbrodt, D.G. (TU Delft BT/Environmental Biotechnology) Kleerebezem, R. (TU Delft BT/Environmental Biotechnology) van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology) Date 2020 Abstract Lactic acid-producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology-based process design and can aid the development of sustainable and energy-efficient bioprocesses. Here we demonstrate the enrichment of lactic acid bacteria in a controlled sequencing batch bioreactor environment using a glucose-based medium supplemented with peptides and B vitamins. A mineral medium enrichment operated in parallel was dominated by Ethanoligenens species and fermented glucose to acetate, butyrate and hydrogen. The complex medium enrichment was populated by Lactococcus, Lactobacillus and Megasphaera species and showed a product spectrum of acetate, ethanol, propionate, butyrate and valerate. An intermediate peak of lactate was observed, showing the simultaneous production and consumption of lactate, which is of concern for lactic acid production purposes. This study underlines that the competitive advantage for lactic acid-producing bacteria primarily lies in their ability to attain a high biomass specific uptake rate of glucose, which was two times higher for the complex medium enrichment when compared to the mineral medium enrichment. The competitive advantage of lactic acid production in rich media can be explained using a resource allocation theory for microbial growth processes. Subject enrichment cultureskineticslactic acid bacteriamicrobial ecologyresource allocation To reference this document use: http://resolver.tudelft.nl/uuid:54930a1d-fb11-443b-8030-46995fa394ff DOI https://doi.org/10.1002/bit.27301 ISSN 0006-3592 Source Biotechnology and Bioengineering, 117 (5), 1281-1293 Part of collection Institutional Repository Document type journal article Rights © 2020 J.L. Rombouts, Elsemiek Madeleine Maria Kranendonk, Alberte Regueira, D.G. Weissbrodt, R. Kleerebezem, Mark C.M. van Loosdrecht Files PDF Rombouts_et_al_2020_Biote ... eering.pdf 3.63 MB Close viewer /islandora/object/uuid:54930a1d-fb11-443b-8030-46995fa394ff/datastream/OBJ/view