Print Email Facebook Twitter Evaluating the Targeting of a Staphylococcus-aureus-Infected Implant with a Radiolabeled Antibody In Vivo Title Evaluating the Targeting of a Staphylococcus-aureus-Infected Implant with a Radiolabeled Antibody In Vivo Author van Dijk, Bruce (University Medical Center Utrecht) Hooning van Duyvenbode, J. Fred F. (University Medical Center Utrecht) de Vor, Lisanne (University Medical Center Utrecht) Nurmohamed, F. Ruben H.A. (University Medical Center Utrecht) Lam, Marnix G.E.H. (University Medical Center Utrecht) Poot, Alex J. (University Medical Center Utrecht) Ramakers, R.M. (TU Delft RST/Technici Pool; MILabs B.V.; University Medical Center Utrecht) Koustoulidou, Sofia (MILabs B.V.; University Medical Center Utrecht) Beekman, F.J. (TU Delft RST/Biomedical Imaging; MILabs B.V.; University Medical Center Utrecht) Weinans, Harrie (TU Delft Biomaterials & Tissue Biomechanics; University Medical Center Utrecht) Date 2023 Abstract Implant infections caused by Staphylococcus aureus are difficult to treat due to biofilm formation, which complicates surgical and antibiotic treatment. We introduce an alternative approach using monoclonal antibodies (mAbs) targeting S. aureus and provide evidence of the specificity and biodistribution of S.-aureus-targeting antibodies in a mouse implant infection model. The monoclonal antibody 4497-IgG1 targeting wall teichoic acid in S. aureus was labeled with indium-111 using CHX-A”-DTPA as a chelator. Single Photon Emission Computed Tomography/computed tomographyscans were performed at 24, 72 and 120 h after administration of the 111In-4497 mAb in Balb/cAnNCrl mice with a subcutaneous implant that was pre-colonized with S. aureus biofilm. The biodistribution of this labelled antibody over various organs was visualized and quantified using SPECT/CT imaging, and was compared to the uptake at the target tissue with the implanted infection. Uptake of the 111In-4497 mAbs at the infected implant gradually increased from 8.34 %ID/cm3 at 24 h to 9.22 %ID/cm3 at 120 h. Uptake at the heart/blood pool decreased over time from 11.60 to 7.58 %ID/cm3, whereas the uptake in the other organs decreased from 7.26 to less than 4.66 %ID/cm3 at 120 h. The effective half-life of 111In-4497 mAbs was determined to be 59 h. In conclusion, 111In-4497 mAbs were found to specifically detect S. aureus and its biofilm with excellent and prolonged accumulation at the site of the colonized implant. Therefore, it has the potential to serve as a drug delivery system for the diagnostic and bactericidal treatment of biofilm. Subject antibodybiodistributioninfectionmiceperiprosthetic joint infectionradioimmunotherapyradiolabelingS. aureusSPECTtheranostics To reference this document use: http://resolver.tudelft.nl/uuid:55a68138-eedb-43e8-a19b-52f9f46577dd DOI https://doi.org/10.3390/ijms24054374 ISSN 1661-6596 Source International Journal of Molecular Sciences (Online), 24 (5) Part of collection Institutional Repository Document type journal article Rights © 2023 Bruce van Dijk, J. Fred F. Hooning van Duyvenbode, Lisanne de Vor, F. Ruben H.A. Nurmohamed, Marnix G.E.H. Lam, Alex J. Poot, R.M. Ramakers, Sofia Koustoulidou, F.J. Beekman, Harrie Weinans, More Authors Files PDF ijms_24_04374_v2.pdf 1.79 MB Close viewer /islandora/object/uuid:55a68138-eedb-43e8-a19b-52f9f46577dd/datastream/OBJ/view