Print Email Facebook Twitter Cell type specificity of glucocorticoid signaling in the adult mouse hippocampus Title Cell type specificity of glucocorticoid signaling in the adult mouse hippocampus Author Viho, Eva M.G. (Leiden University Medical Center) Buurstede, Jacobus C. (Leiden University Medical Center) Berkhout, Jari B. (Leiden University Medical Center) Mahfouz, A.M.E.T.A. (TU Delft Pattern Recognition and Bioinformatics; Leiden University Medical Center) Meijer, Onno C. (Leiden University Medical Center) Date 2021 Abstract Glucocorticoid stress hormones are powerful modulators of brain function and can affect mood and cognitive processes. The hippocampus is a prominent glucocorticoid target and expresses both the glucocorticoid receptor (GR: Nr3c1) and the mineralocorticoid receptor (MR: Nr3c2). These nuclear steroid receptors act as ligand-dependent transcription factors. Transcriptional effects of glucocorticoids have often been deduced from bulk mRNA measurements or spatially informed individual gene expression. However, only sparse data exists allowing insights on glucocorticoid-driven gene transcription at the cell type level. Here, we used publicly available single-cell RNA sequencing data to assess the cell-type specificity of GR and MR signaling in the adult mouse hippocampus. The data confirmed that Nr3c1 and Nr3c2 expression differs across neuronal and non-neuronal cell populations. We analyzed co-expression with sex hormones receptors, transcriptional coregulators, and receptors for neurotransmitters and neuropeptides. Our results provide insights in the cellular basis of previous bulk mRNA results and allow the formulation of more defined hypotheses on the effects of glucocorticoids on hippocampal function. Subject corticosteroid receptorshippocampussingle-cell RNA sequencingstress hormonestranscription regulation To reference this document use: http://resolver.tudelft.nl/uuid:6e0c1591-9cc2-4fcc-b1d9-a77b4ad98b4f DOI https://doi.org/10.1111/jne.13072 ISSN 0953-8194 Source Journal of Neuroendocrinology, 34 (2022) (2) Part of collection Institutional Repository Document type journal article Rights © 2021 Eva M.G. Viho, Jacobus C. Buurstede, Jari B. Berkhout, A.M.E.T.A. Mahfouz, Onno C. Meijer Files PDF J_Neuroendocrinology_2021 ... campus.pdf 3.51 MB Close viewer /islandora/object/uuid:6e0c1591-9cc2-4fcc-b1d9-a77b4ad98b4f/datastream/OBJ/view