Print Email Facebook Twitter Robust experimental methods to study in-vivo pre-steady state kinetics of primary metabolism in Saccharomyces cerevisiae Title Robust experimental methods to study in-vivo pre-steady state kinetics of primary metabolism in Saccharomyces cerevisiae Author Mashego, M.R. Contributor Heijnen, J.J. (promotor) Faculty Applied Sciences Date 2005-11-03 Abstract In this thesis, robust experimental methods and experimental designs for quantitative analysis of the metabolome (concentrations of relevant metabolites in the cell) of Saccharomyces cerevisiae (Bakers yeast) have been presented. In this research, it is essential to obtain a snapshot of intracellular as well as extracellular metabolite concentrations under defined conditions and as a function of time. Therefore, it is necessary to obtain within a fraction of a second, a representative sample from the population of cells and instantly arrest cellular metabolism. Subject saccharomyces cerevisiaechemostat culturemetabolomicsglucose perturbationsbioscope To reference this document use: http://resolver.tudelft.nl/uuid:86b788f7-277e-47c6-8a4e-43e139e039c6 ISBN 90-902-0060-6 Part of collection Institutional Repository Document type doctoral thesis Rights (c) 2005 M.R. Mashego Files PDF as_mashego_20051103.pdf 3.24 MB Close viewer /islandora/object/uuid:86b788f7-277e-47c6-8a4e-43e139e039c6/datastream/OBJ/view