Print Email Facebook Twitter Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast Title Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast Author van Aalst, A.C.A. (TU Delft BT/Industriele Microbiologie) Contributor Pronk, J.T. (promotor) Daran, J.G. (promotor) Degree granting institution Delft University of Technology Date 2023-10-02 Abstract The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of yeast biomass and glycerol are coupled via redox-cofactor balances, as a net generation of NADH during biomass formation needs to be compensated by NADH-dependent formation of glycerol from sugar. This thesis discusses redox-engineering strategies for maximizing ethanol yields on substrate. Subject Redox engineeringEthanol productionSaccharomyces cerevisiaeco-cultureMetabolic engineeringanaerobic fermentationElectronsindustrial biotechnology To reference this document use: https://doi.org/10.4233/uuid:9a149309-2339-40c5-9151-032d70b91dbe ISBN 978-94-6483-352-2 Part of collection Institutional Repository Document type doctoral thesis Rights © 2023 A.C.A. van Aalst Files PDF Aafke_van_Aalst_dissertation.pdf 61.48 MB Close viewer /islandora/object/uuid:9a149309-2339-40c5-9151-032d70b91dbe/datastream/OBJ/view