Print Email Facebook Twitter Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples Title Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples Author Yin, Xiaole (The University of Hong Kong) Chen, Xi (The University of Hong Kong) Jiang, Xiao Tao (University of New South Wales Faculty of Medicine) Yang, Ying (Sun Yat-sen University) Li, Bing (Tsinghua University) Shum, Marcus Ho Hin (University of Hong Kong) Schmitt, H. (TU Delft BT/Environmental Biotechnology; Rijksinstituut voor Volksgezondheid en Milieu (RIVM)) Cha, Chang Jun (Chung-Ang University) van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology) Date 2023 Abstract Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys in human and animal sectors under the "One-Health"framework. However, there are substantial challenges in comparing and synthesizing the results of multiple studies that employ different test methods and approaches in bioinformatic analysis. In this article, we consider the commonly used quantification units (ARG copy per cell, ARG copy per genome, ARG density, ARG copy per 16S rRNA gene, RPKM, coverage, PPM, etc.) for profiling ARGs and suggest a universal unit (ARG copy per cell) for reporting such biological measurements of samples and improving the comparability of different surveillance efforts. Subject ARG copy per celllong-read sequencingmetagenomicsone-healthstandardization To reference this document use: http://resolver.tudelft.nl/uuid:9b92694f-fca1-4dc6-ba7d-3fc91d31b68e DOI https://doi.org/10.1021/acs.est.3c00159 Embargo date 2024-01-13 ISSN 0013-936X Source Environmental Science & Technology (Washington), 57 (26), 9713-9721 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2023 Xiaole Yin, Xi Chen, Xiao Tao Jiang, Ying Yang, Bing Li, Marcus Ho Hin Shum, H. Schmitt, Chang Jun Cha, Mark C.M. van Loosdrecht, More Authors Files PDF acs.est.3c00159.pdf 2.16 MB Close viewer /islandora/object/uuid:9b92694f-fca1-4dc6-ba7d-3fc91d31b68e/datastream/OBJ/view