Fluid-crystal coexistence for proteins and inorganic nanocolloids: Dependence on ionic strength

Fluid-crystal coexistence for proteins and inorganic nanocolloids: Dependence on ionic strength

Prinsen, P.
Odijk, T.

Applied Sciences

2006-08-18

We investigate theoretically the fluid-crystal coexistence of solutions of globular charged nanoparticles such as proteins and inorganic colloids. The thermodynamic properties of the fluid phase are computed via the optimized Baxter model P. Prinsen and T. Odijk [J. Chem. Phys. 121, 6525 (2004)]. This is done specifically for lysozyme and silicotungstates for which the bare adhesion parameters are evaluated via the experimental second virial coefficients. The electrostatic free energy of the crystal is approximated by supposing the cavities in the interstitial phase between the particles are spherical in form. In the salt-free case a Poisson-Boltzmann equation is solved to calculate the effective charge on a particle and a Donnan approximation is used to derive the chemical potential and osmotic pressure in the presence of salt. The coexistence data of lysozyme and silicotungstates are analyzed within this scheme, especially with regard to the ionic-strength dependence of the chemical potentials. The latter agree within the two phases provided some upward adjustment of the effective charge is allowed for.

crystallisation
proteins
nanoparticles
colloids
equations of state
Poisson equation
Boltzmann equation
chemical potential
osmosis
interstitials

http://resolver.tudelft.nl/uuid:9c61f7ec-7bd0-480a-8087-6214fcd41b8f

https://doi.org/10.1063/1.2336423

American Institute of Physics

http://link.aip.org/link/JCPSA6/v125/i7/p074903/s1

Journal of Chemical Physics, 125 (7), 2006

Institutional Repository

journal article

(c) 2006 Prinsen, P.; Odijk, T.; American Institute of Physics