Print Email Facebook Twitter 3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells Title 3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells Author Barin, N. (TU Delft Micro and Nano Engineering; Erasmus MC) Balcioglu, Hayri E. (Erasmus MC) de Heer, Iris (Erasmus MC) de Wit, Maurice (Erasmus MC) Lamfers, Martine L.M. (Erasmus MC) van Royen, Martin E. (Erasmus MC) French, Pim J. (Erasmus MC) Accardo, A. (TU Delft Micro and Nano Engineering) Date 2022 Abstract A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy, and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient-derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks is observed for cells cultured on the scaffolds. The 3D-engineered glioma scaffolds therefore represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies. Subject 2-photon polymerization3D cell culturebrain cancerepidermal growth factor receptormicrotubes To reference this document use: http://resolver.tudelft.nl/uuid:a20671eb-b256-4848-97aa-f5a9aabd666f DOI https://doi.org/10.1002/smll.202204485 ISSN 1613-6810 Source Small (online), 18 (49) Part of collection Institutional Repository Document type journal article Rights © 2022 N. Barin, Hayri E. Balcioglu, Iris de Heer, Maurice de Wit, Martine L.M. Lamfers, Martin E. van Royen, Pim J. French, A. Accardo Files PDF Small_2022_Barin_3D_E2_80 ... tor_in.pdf 3.8 MB Close viewer /islandora/object/uuid:a20671eb-b256-4848-97aa-f5a9aabd666f/datastream/OBJ/view