Print Email Facebook Twitter A robotic platform to screen aqueous two-phase systems for overcoming inhibition in enzymatic reactions Title A robotic platform to screen aqueous two-phase systems for overcoming inhibition in enzymatic reactions Author Consorti Bussamra, B. (TU Delft BT/Bioprocess Engineering; University of Campinas) Gomes, Joana Castro (Student TU Delft) Freitas, Sindelia (University of Campinas) Mussatto, Solange I. (Technical University of Denmark) da Costa, Aline Carvalho (University of Campinas) van der Wielen, L.A.M. (TU Delft BT/Bioprocess Engineering; University of Limerick) Ottens, M. (TU Delft BT/Bioprocess Engineering) Date 2019 Abstract Aqueous two-phase systems (ATPS) can be applied to enzymatic reactions that are affected by product inhibition. In the biorefinery context, sugars inhibit the cellulolytic enzymes in charge of converting the biomass. Here, we present a strategy to select an ATPS (formed by polymer and salt) that can separate sugar and enzymes. This automated and miniaturized method is able to determine phase diagrams and partition coefficients of solutes in these. Tailored approaches to quantify the solutes are presented, taking into account the limitations of techniques that can be applied with ATPS due to the interference of phase forming components with the analytics. The developed high-throughput (HT) platform identifies suitable phase forming components and the tie line of operation. This fast methodology proposes to screen up to six different polymer-salt systems in eight days and supplies the results to understand the influence of sugar and protein concentrations on their partition coefficients. Subject Aqueous two-phase system (ATPS)Glucose partitioningHigh-throughput screeningProduct inhibitionProtein partitioning To reference this document use: http://resolver.tudelft.nl/uuid:a6a51aba-dbde-4d71-8159-15090ac02d09 DOI https://doi.org/10.1016/j.biortech.2019.01.136 Embargo date 2021-02-10 ISSN 0960-8524 Source Bioresource Technology, 280, 37-50 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2019 B. Consorti Bussamra, Joana Castro Gomes, Sindelia Freitas, Solange I. Mussatto, Aline Carvalho da Costa, L.A.M. van der Wielen, M. Ottens Files PDF Bussamra_et_al_2019_Post_Print.pdf 2.24 MB Close viewer /islandora/object/uuid:a6a51aba-dbde-4d71-8159-15090ac02d09/datastream/OBJ/view