Print Email Facebook Twitter Effects of diabetes mellitus on fibrin clot structure and mechanics in a model of acute neutrophil extracellular traps (Nets) formation Title Effects of diabetes mellitus on fibrin clot structure and mechanics in a model of acute neutrophil extracellular traps (Nets) formation Author de Vries, J.J. (Erasmus MC) Hoppenbrouwers, Tamara (Erasmus MC; Wageningen University & Research) Martinez Torres, C.E. (TU Delft BN/Gijsje Koenderink Lab; Kavli institute of nanoscience Delft; AMOLF) Majied, Rezin (Erasmus MC) Özcan, Behiye (Erasmus MC) Hoek, Mandy van (Erasmus MC) Leebeek, Frank W.G. (Erasmus MC) Rijken, Dingeman C. (Erasmus MC) Koenderink, G.H. (TU Delft BN/Gijsje Koenderink Lab; Kavli institute of nanoscience Delft; AMOLF) Date 2020 Abstract Subjects with diabetes mellitus (DM) have an increased risk of arterial thrombosis, to which changes in clot structure and mechanics may contribute. Another contributing factor might be an increased formation of neutrophil extracellular traps (NETs) in DM. NETs are mainly formed during the acute phase of disease and form a network within the fibrin matrix, thereby influencing clot properties. Previous research has shown separate effects of NETs and DM on clot properties, therefore our aim was to study how DM affects clot properties in a model resembling an acute phase of disease with NETs formation. Clots were prepared from citrated plasma from subjects with and without DM with the addition of NETs, induced in neutrophils by S. aureus bacteria or phorbol myristate acetate (PMA). Structural parameters were measured using scanning electron microscopy, mechanical properties using rheology, and sensitivity to lysis using a fluorescence-based fibrinolysis assay. Plasma clots from subjects with DM had significantly thicker fibers and fewer pores and branch points than clots from subjects without DM. In addition, fibrinolysis was significantly slower, while mechanical properties were similar between both groups. In conclusion, in a model of acute NETs formation, DM plasma shows prothrombotic effects on fibrin clots. Subject Arterial thrombosisDiabetes mellitusFibrinFibrinolysisNeutrophil extracellular traps To reference this document use: http://resolver.tudelft.nl/uuid:a743db23-e77d-4288-ad02-e667837c676e DOI https://doi.org/10.3390/ijms21197107 ISSN 1661-6596 Source International Journal of Molecular Sciences (Online), 21 (19), 1-15 Part of collection Institutional Repository Document type journal article Rights © 2020 J.J. de Vries, Tamara Hoppenbrouwers, C.E. Martinez Torres, Rezin Majied, Behiye Özcan, Mandy van Hoek, Frank W.G. Leebeek, Dingeman C. Rijken, G.H. Koenderink, More Authors Files PDF ijms_21_07107.pdf 2.46 MB Close viewer /islandora/object/uuid:a743db23-e77d-4288-ad02-e667837c676e/datastream/OBJ/view