Print Email Facebook Twitter U-SPECT-II: An Ultra-High-Resolution Device for Molecular Small-Animal Imaging Title U-SPECT-II: An Ultra-High-Resolution Device for Molecular Small-Animal Imaging Author Van der Have, F. Vastenhouw, B. Ramakers, R.M. Branderhorst, W. Krah, J.O. Ji, C. Staelens, S.G. Beekman, F.J. Faculty Applied Sciences Department RRR/Radiation, Radionuclides and Reactors Date 2009-01-01 Abstract We present a new rodent SPECT system (U-SPECT-II) that enables molecular imaging of murine organs down to resolutions of less than half a millimeter and high-resolution total-body imaging. Methods: The U-SPECT-II is based on a triangular stationary detector set-up, an XYZ stage that moves the animal during scanning, and interchangeable cylindric collimators (each containing 75 pinhole apertures) for both mouse and rat imaging. A novel graphical user interface incorporating preselection of the field of view with the aid of optical images of the animal focuses the pinholes to the area of interest, thereby maximizing sensitivity for the task at hand. Images are obtained from list mode data using statistical reconstruction that takes system blurring into account to increase resolution. Results: For 99mTc, resolutions determined with capillary phantoms were smaller than 0.35 and 0.45 mm using the mouse collimator with 0.35- and 0.6-mm pinholes, respectively, and less than 0.8mm using the rat collimator with 1.0-mm pinholes. Peak geometric sensitivity is 0.07% and 0.18% for the mouse collimator with 0.35- and 0.6-mm pinholes, respectively, and 0.09% for the rat collimator. Resolution with 111In, compared with that with 99mTc, was barely degraded, and resolution with 125I was degraded by about 10%, with some additional distortion. In vivo, kidney, tumor, and bone images illustrated that U-SPECT-II could be used for novel applications in the study of dynamic biologic systems and radiopharmaceuticals at the suborgan level. Conclusion: Images and movies obtained with U-SPECT-II provide high-resolution radiomolecule visualization in rodents. Discrimination of molecule concentrations between adjacent volumes of about 0.04 µL in mice and 0.5 µL in rats with U-SPECT-II is readily possible. Subject molecular imagingpinholeSPECTsmall animalreconstruction To reference this document use: http://resolver.tudelft.nl/uuid:aeaaa6a0-0583-456f-83b9-774f2d5cc64e DOI https://doi.org/10.2967/jnumed.108.056606 Publisher Society of Nuclear Medicine ISSN 0161-5505 Source Journal of Nuclear Medicine, 50 (4), 2009 Part of collection Institutional Repository Document type journal article Rights (c) 2009 The Author(s)Society of Nuclear Medicine Files PDF Beekman3_2009.pdf 1.04 MB Close viewer /islandora/object/uuid:aeaaa6a0-0583-456f-83b9-774f2d5cc64e/datastream/OBJ/view