Print Email Facebook Twitter Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction Title Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction Author Winglee, K. McGuire, A.M. Maiga, M. Abeel, T.E.P.M.F. Shea, T. Desjardins, C.A. Diarra, B. Baya, B. Sanogo, M. Diallo, S. Earl, A. Bishai, W.R. Faculty Electrical Engineering, Mathematics and Computer Science Department Intelligent Systems Date 2016-01-11 Abstract Background Mycobacterium africanum, made up of lineages 5 and 6 within the Mycobacterium tuberculosis complex (MTC), causes up to half of all tuberculosis cases in West Africa, but is rarely found outside of this region. The reasons for this geographical restriction remain unknown. Possible reasons include a geographically restricted animal reservoir, a unique preference for hosts of West African ethnicity, and an inability to compete with other lineages outside of West Africa. These latter two hypotheses could be caused by loss of fitness or altered interactions with the host immune system. Methodology/Principal Findings We sequenced 92 MTC clinical isolates from Mali, including two lineage 5 and 24 lineage 6 strains. Our genome sequencing assembly, alignment, phylogeny and average nucleotide identity analyses enabled us to identify features that typify lineages 5 and 6 and made clear that these lineages do not constitute a distinct species within the MTC. We found that in Mali, lineage 6 and lineage 4 strains have similar levels of diversity and evolve drug resistance through similar mechanisms. In the process, we identified a putative novel streptomycin resistance mutation. In addition, we found evidence of person-to-person transmission of lineage 6 isolates and showed that lineage 6 is not enriched for mutations in virulence-associated genes. Conclusions This is the largest collection of lineage 5 and 6 whole genome sequences to date, and our assembly and alignment data provide valuable insights into what distinguishes these lineages from other MTC lineages. Lineages 5 and 6 do not appear to be geographically restricted due to an inability to transmit between West African hosts or to an elevated number of mutations in virulence-associated genes. However, lineage-specific mutations, such as mutations in cell wall structure, secretion systems and cofactor biosynthesis, provide alternative mechanisms that may lead to host specificity. To reference this document use: http://resolver.tudelft.nl/uuid:bc7a42bb-e99b-4a27-848f-29ae48ac1bb1 Publisher Public Library of Science ISSN 1935-2735 Source https://doi.org/10.1371/journal.pntd.0004332 Source PLoS Neglected Tropical Diseases, 10 (1), 2016 Part of collection Institutional Repository Document type journal article Rights © 2016 The Author(s)This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Files PDF 329667.pdf 2.19 MB Close viewer /islandora/object/uuid:bc7a42bb-e99b-4a27-848f-29ae48ac1bb1/datastream/OBJ/view