Senescence during early differentiation reduced the chondrogenic differentiation capacity of mesenchymal progenitor cells

Senescence during early differentiation reduced the chondrogenic differentiation capacity of mesenchymal progenitor cells

Voskamp, Chantal (Erasmus MC)
Koevoet, Wendy J.L.M. (Erasmus MC)
van Osch, G.J.V.M. (TU Delft Biomaterials & Tissue Biomechanics; Erasmus MC)
Narcisi, Roberto (Erasmus MC)

2023

Introduction: Mesenchymal stromal/progenitor cells (MSCs) are promising for cartilage cell-based therapies due to their chondrogenic differentiation capacity. However, MSCs can become senescent during in vitro expansion, a state characterized by stable cell cycle arrest, metabolic alterations, and substantial changes in the gene expression and secretory profile of the cell. In this study, we aimed to investigate how senescence and the senescence-associated secretory phenotype (SASP) affect chondrogenic differentiation of MSCs.

Methods: To study the effect of senescence, we exposed MSCs to gamma irradiation during expansion or during chondrogenic differentiation (the pellet culture). Western blot analysis was used to evaluate MSCs response to the chondrogenic inductor TGF-β.

Results: When senescence was induced during expansion or at day 7 of chondrogenic differentiation, we observed a significant reduction in the cartilage matrix. Interestingly, when senescence was induced at day 14 of differentiation, chondrogenesis was not significantly altered. Moreover, exposing chondrogenic pellets to the medium conditioned by senescent pellets had no significant effect on the expression of anabolic or catabolic cartilage markers, suggesting a neglectable paracrine effect of senescence on cartilage generation in our model. Finally, we show that senescent MSCs showed lower phosphorylated SMAD2 levels after TGFβ1 stimulation than control MSCs.

Conclusion: Overall, these results suggest that the occurrence of senescence in MSCs during expansion or early differentiation could be detrimental for cartilage tissue engineering.

senescence
cartilage
MSC
chondrogenesis
SASP
regenerative medicine
tissue engineering
TGFβ

http://resolver.tudelft.nl/uuid:c6646856-9d0b-435b-a576-4a15bd3fcfc0

https://doi.org/10.3389/fbioe.2023.1241338

2296-4185

Frontiers in Bioengineering and Biotechnology, 11

Institutional Repository

journal article

© 2023 Chantal Voskamp, Wendy J.L.M. Koevoet, G.J.V.M. van Osch, Roberto Narcisi