Print Email Facebook Twitter Force-free measurements of the conformations of DNA molecules tethered to a wall Title Force-free measurements of the conformations of DNA molecules tethered to a wall Author Lindner, M. Nir, G. Medalion, S. Dietrich, H.R.C. Rabin, Y. Garini, Y. Faculty Applied Sciences Department BT/Biotechnology Date 2011-01-27 Abstract Using an optimized combination of tethered particle motion method, total internal reflection, and a gold nanobead, we measured the three-dimensional distribution of the free end of a tethered DNA molecule. The distribution along the axial z direction (perpendicular to the surface) is found to be Rayleigh-like, in agreement with wormlike chain and freely jointed chain simulations. Using these simulations, we show that the presence of the wall increases the correlations between the orientations of neighboring chain segments compared to free DNA. While the measured and the simulated planar (xy) distributions always agree with that of a Gaussian-random-walk (GRW) model, for short DNA lengths (1 ?m) studied in our experiment, the corresponding axial (z) distributions deviate from those predicted for a GRW confined to half-space. To reference this document use: http://resolver.tudelft.nl/uuid:ea8a1594-f32b-42f9-903c-e5a9cea8c5af DOI https://doi.org/10.1103/PhysRevE.83.011916 Publisher American Physical Society ISSN 1539-3755 Source Physical Review E, 83 (1), 2011 Part of collection Institutional Repository Document type journal article Rights (c) 2011 The Author(s)American Physical Society Files PDF Dietrich2011.pdf 556.53 KB Close viewer /islandora/object/uuid:ea8a1594-f32b-42f9-903c-e5a9cea8c5af/datastream/OBJ/view