Print Email Facebook Twitter Micro-Vessels-Like 3D Scaffolds for Studying the Proton Radiobiology of Glioblastoma-Endothelial Cells Co-Culture Models Title Micro-Vessels-Like 3D Scaffolds for Studying the Proton Radiobiology of Glioblastoma-Endothelial Cells Co-Culture Models Author Akolawala, Q. (TU Delft Micro and Nano Engineering; Holland Particle Therapy Centre) Keuning, F.E. (Erasmus Universiteit Rotterdam) Rovituso, M. (Holland Particle Therapy Centre) van Burik, Wouter (Holland Particle Therapy Centre) van der Wal, E.M. (Holland Particle Therapy Centre) Versteeg, Henri H. (Leiden University Medical Center) Rondon, Araci M.R. (Leiden University Medical Center) Accardo, A. (TU Delft Micro and Nano Engineering) Date 2023 Abstract Glioblastoma (GBM) is a devastating cancer of the brain with an extremely poor prognosis. While X-ray radiotherapy and chemotherapy remain the current standard, proton beam therapy is an appealing alternative as protons can damage cancer cells while sparing the surrounding healthy tissue. However, the effects of protons on in vitro GBM models at the cellular level, especially when co-cultured with endothelial cells, the building blocks of brain micro-vessels, are still unexplored. In this work, novel 3D-engineered scaffolds inspired by the geometry of brain microvasculature are designed, where GBM cells cluster and proliferate. The architectures are fabricated by two-photon polymerization (2PP), pre-cultured with endothelial cells (HUVECs), and then cultured with a human GBM cell line (U251). The micro-vessel structures enable GBM in vivo-like morphologies, and the results show a higher DNA double-strand breakage in GBM monoculture samples when compared to the U251/HUVECs co-culture, with cells in 2D featuring a larger number of DNA damage foci when compared to cells in 3D. The discrepancy in terms of proton radiation response indicates a difference in the radioresistance of the GBM cells mediated by the presence of HUVECs and the possible induction of stemness features that contribute to radioresistance and improved DNA repair. Subject endothelial cellsengineered cell microenvironmentsglioblastomaproton therapytwo-photon polymerization To reference this document use: http://resolver.tudelft.nl/uuid:fdacfae6-9e40-4ff0-8349-92f4dc5a23ec DOI https://doi.org/10.1002/adhm.202302988 ISSN 2192-2640 Source Advanced Healthcare Materials, 13 (2024) (6) Part of collection Institutional Repository Document type journal article Rights © 2023 Q. Akolawala, F.E. Keuning, M. Rovituso, Wouter van Burik, E.M. van der Wal, Henri H. Versteeg, Araci M.R. Rondon, A. Accardo Files PDF Adv_Healthcare_Materials_ ... ogy_of.pdf 12.41 MB Close viewer /islandora/object/uuid:fdacfae6-9e40-4ff0-8349-92f4dc5a23ec/datastream/OBJ/view