Re-scan confocal microscopy: Scanning twice for better resolution

Title

Re-scan confocal microscopy: Scanning twice for better resolution

 Author

De Luca, G.M.R.
Breedijk, R.M.P.
Brandt, R.A.J.
Zeelenberg, C.H.C.
De Jong, B.E.
Timmermans, W.
Nahidi Azar, L.
Hoebe, R.A.
Stallinga, S.
Manders, E.M.M.

Faculty

Applied Sciences

 Department

IST/Imaging Science and Technology

 Date

2013-10-25

 Abstract

We present a new super-resolution technique, Re-scan Confocal Microscopy (RCM), based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD-camera. This new microscope has improved lateral resolution and strongly improved sensitivity while maintaining the sectioning capability of a standard confocal microscope. This simple technology is typically useful for biological applications where the combination high-resolution and high-sensitivity is required.

 To reference this document use:

http://resolver.tudelft.nl/uuid:03f51884-8326-48a6-9437-414b553ea420

 DOI

https://doi.org/10.1364/BOE.4.002644

 Publisher

Optical Society of America

 ISSN

2156-7085

 Source

http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-11-2644

 Source

Biomedical Optics Express, 4 (11), 2013

 Part of collection

Institutional Repository

 Document type

journal article

 Rights

© 2013 Optical Society of America
Files
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