Title
Visualizing Dynamic Changes During TGF-β-Induced Epithelial to Mesenchymal Transition
Author
Sinha, Abhishek (Leiden University Medical Center)
Mehta, P.P. (TU Delft ChemE/Product and Process Engineering; Leiden University Medical Center)
Fan, Chuannan (Leiden University Medical Center)
Zhang, Jing (Leiden University Medical Center)
Marvin, Dieuwke L. (Leiden University Medical Center)
van Dinther, Maarten (Leiden University Medical Center)
Ritsma, Laila (Leiden University Medical Center)
Boukany, P. (TU Delft ChemE/Product and Process Engineering) 
ten Dijke, Peter (Leiden University Medical Center)
Contributor
Zi, Zhike (editor)
Liu, Xuedong (editor)
Date
2022
Abstract
Epithelial to mesenchymal transition (EMT) is crucial during embryonic development, tissue fibrosis, and cancer progression. Epithelial cells that display a cobblestone-like morphology can undergo a switch to mesenchymal-like phenotype, displaying an elongated spindle shape or a fibroblast-like morphology. EMT is characterized by timely and reversible alterations of molecular and cellular processes. The changes include loss of epithelial and gain of mesenchymal marker expression, loss of polarity, increased cell migratory and invasive properties. Epithelial cells can progress unevenly during this transition and attain hybrid E/M states or metastable EMT states, referred to as epithelial cell plasticity. To gain a deeper insight into the mechanism of EMT, understanding the dynamic aspects of this process is essential. One of the most prominent factors to induce EMT is the cytokine transforming growth factor-β (TGF-β). This chapter discusses molecular and cellular techniques to monitor TGF-β-induced signaling and EMT changes in normal and cancer cell lines. These methods include measuring the TGF-β-induced activation of its intracellular SMAD effectors proteins and changes in epithelial/mesenchymal marker expression and localization. Moreover, we describe assays of cell migration and dynamic reorganization of the actin cytoskeleton and stress filaments that are frequently part of the TGF-β-induced EMT cellular response.
Subject
Cell migration
EMP
EMT
Mesenchymal
Metastasis
TGF-β
To reference this document use:
http://resolver.tudelft.nl/uuid:306e0875-47d7-46ef-92a7-b81e5b476682
DOI
https://doi.org/10.1007/978-1-0716-2277-3_5
Publisher
Humana Press Inc.
Embargo date
2023-07-01
Source
Methods in Molecular Biology
Series
Methods in Molecular Biology, 1064-3745, 2488
Bibliographical note
Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
Part of collection
Institutional Repository
Document type
book chapter
Rights
© 2022 Abhishek Sinha, P.P. Mehta, Chuannan Fan, Jing Zhang, Dieuwke L. Marvin, Maarten van Dinther, Laila Ritsma, P. Boukany, Peter ten Dijke