Print Email Facebook Twitter Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis Title Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis Author Tai, S.L. Daran-Lapujade, P. Walsh, M.C. Pronk, J.T. Daran, J.M. Faculty Applied Sciences Department Biotechnology Date 2007-10-10 Abstract Effects of suboptimal temperatures on transcriptional regulation in yeast have been extensively studied in batch cultures. To eliminate indirect effects of specific growth rates that are inherent to batch-cultivation studies, genome-wide transcriptional responses to low temperatures were analyzed in steady-state chemostats, grown at a fixed specific growth rate (0.03 h-1). Although in vivo metabolic fluxes were essentially the same in cultures grown at 12 and at 30°C, concentrations of the growth-limiting nutrients (glucose or ammonia) were higher at 12°C. This difference was reflected by transcript levels of genes that encode transporters for the growth-limiting nutrients. Several transcriptional responses to low temperature occurred under both nutrient-limitation regimes. Increased transcription of ribosome-biogenesis genes emphasized the importance of adapting protein-synthesis capacity to low temperature. In contrast to observations in cold-shock and batch-culture studies, transcript levels of environmental stress response genes were reduced at 12°C. Transcription of trehalose-biosynthesis genes and intracellular trehalose levels indicated that, in contrast to its role in cold-shock adaptation, trehalose is not involved in steady-state low-temperature adaptation. Comparison of the chemostat- based transcriptome data with literature data revealed large differences between transcriptional reprogramming during long-term low-temperature acclimation and the transcriptional responses to a rapid transition to low temperature. To reference this document use: http://resolver.tudelft.nl/uuid:3809cf80-d848-478a-82e5-49c1e42725d4 Publisher American Society for Cell Biology ISSN 1059-1524 Source Molecular Biology of the Cell, 18 (12), 2007 Part of collection Institutional Repository Document type journal article Rights (c) 2007 Tai, S.L. et al. ; The American Society for Cell Biology Files PDF Tai_2007.pdf 1.84 MB Close viewer /islandora/object/uuid:3809cf80-d848-478a-82e5-49c1e42725d4/datastream/OBJ/view