Print Email Facebook Twitter Crystal ball - 2013: Recombination-based DNA assembly in metabolic engineering: a goodbye to old workhorses? Title Crystal ball - 2013: Recombination-based DNA assembly in metabolic engineering: a goodbye to old workhorses? Author Daran, J.M. Pronk, J.T. Faculty Applied Sciences Department BT/Biotechnology Date 2012-12-18 Abstract In this feature, leading researchers in the field of environmental microbiology speculate on the technical and conceptual developments that will drive innovative research and open new vistas over the next few years. For the better part of four decades, genetic engineering has relied on a universal toolbox containing three indispensable implements: restriction enzymes, ligases and, last but not least, Escherichia coli. Even those of us who now languish behind laptops and in meetings rather than work at the bench instantaneously recognize the smells of Luria broth and plasmid preps. To reference this document use: http://resolver.tudelft.nl/uuid:7ad7c09f-c259-4ebb-86ac-2cdce5e8a376 Publisher Society for Applied Microbiology and Blackwell Publishing ISSN 1751-7907 Source Microbial Biotechnology, 6 (1), 2012 Part of collection Institutional Repository Document type journal article Rights © 2012 The Author(s)This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited Files PDF Daran.pdf 31.87 KB Close viewer /islandora/object/uuid:7ad7c09f-c259-4ebb-86ac-2cdce5e8a376/datastream/OBJ/view