On anammox activity at low temperature

On anammox activity at low temperature: Effect of ladderane composition and process conditions

Kouba, Vojtěch (University of Chemistry and Technology Prague)
Hůrková, Kamila (University of Chemistry and Technology Prague)
Navrátilová, Klára (University of Chemistry and Technology Prague)
Kok, Dana (University of Chemistry and Technology Prague)
Benáková, Andrea (University of Chemistry and Technology Prague)
Laureni, M. (TU Delft Sanitary Engineering)
Vodičková, Patricie (University of Chemistry and Technology Prague)
van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology)
Weissbrodt, D.G. (TU Delft BT/Environmental Biotechnology)

2022

The application of partial nitritation-anammox (PN/A) under mainstream conditions can enable substantial cost savings at wastewater treatment plants (WWTPs), but how process conditions and cell physiology affect anammox performance at psychrophilic temperatures below 15 °C remains poorly understood. We tested 14 anammox communities, including 8 from globally-installed PN/A processes, for (i) specific activity at 10–30 °C, (ii) composition of membrane lipids, and (iii) microbial community structure. We observed that membrane composition and cultivation temperature were closely related to the activity of anammox biomasses. The size of ladderane lipids and the content of bacteriohopanoids were key physiological components related to anammox performance at low temperatures. We also indicate that the adaptation of mesophilic cultures to psychrophilic regime necessitates months, but in some cases can take up to 5 years. Interestingly, biomass enriched in the marine genus “Candidatus Scalindua” displayed outstanding potential for nitrogen removal from cold streams. Collectively, our comprehensive study provides essential knowledge of cold adaptation mechanism, will enable more accurate modelling and suggests highly promising target anammox genera for inoculation and set-up of anammox reactors, in particular for mainstream WWTPs.

Activation energy
Adaptation to low temperature
Anaerobic ammonium oxidation
Anammox activity
Anammox genus
Ladderane phospholipid

http://resolver.tudelft.nl/uuid:8dad0a14-b29e-468f-8543-90e043516b00

https://doi.org/10.1016/j.cej.2022.136712

2022-11-02

1385-8947

Chemical Engineering Journal, 445

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

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© 2022 Vojtěch Kouba, Kamila Hůrková, Klára Navrátilová, Dana Kok, Andrea Benáková, M. Laureni, Patricie Vodičková, Mark C.M. van Loosdrecht, D.G. Weissbrodt, More Authors