Print Email Facebook Twitter A culture model to analyze the acute biomaterial-dependent reaction of human primary neutrophils in vitro Title A culture model to analyze the acute biomaterial-dependent reaction of human primary neutrophils in vitro Author Wesdorp, Marinus A. (Erasmus MC; AO Foundation) Schwab, Andrea (Erasmus MC; AO Foundation) Bektas, Ezgi Irem (AO Foundation) Narcisi, Roberto (Erasmus MC) Eglin, David (University of Twente; AO Foundation; Université de Lyon) Stoddart, Martin J. (AO Foundation) van Osch, G.J.V.M. (TU Delft Biomaterials & Tissue Biomechanics; Erasmus MC) D'Este, Matteo (AO Foundation) Date 2023 Abstract Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials, the onset and resolution of chronic inflammation, and macrophage polarization. However, the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood. Here, we report an in vitro culture model that comprehensively describes the most important neutrophil functions in the light of tissue repair. We isolated human primary neutrophils from peripheral blood and exposed them to a panel of hard, soft, naturally- and synthetically-derived materials. The overall trend showed increased neutrophil survival on naturally derived constructs, together with higher oxidative burst, decreased myeloperoxidase and neutrophil elastase and decreased cytokine secretion compared to neutrophils on synthetic materials. The culture model is a step to better understand the immune modulation elicited by biomaterials. Further studies are needed to correlate the neutrophil response to tissue healing and to elucidate the mechanism triggering the cell response and their consequences in determining inflammation onset and resolution. To reference this document use: http://resolver.tudelft.nl/uuid:8f19f6af-d566-4078-8386-a9c51809a55a DOI https://doi.org/10.1016/j.bioactmat.2022.05.036 ISSN 2452-199X Source Bioactive Materials, 20, 627-637 Part of collection Institutional Repository Document type journal article Rights © 2023 Marinus A. Wesdorp, Andrea Schwab, Ezgi Irem Bektas, Roberto Narcisi, David Eglin, Martin J. Stoddart, G.J.V.M. van Osch, Matteo D'Este Files PDF 1_s2.0_S2452199X22002584_main.pdf 5.19 MB Close viewer /islandora/object/uuid:8f19f6af-d566-4078-8386-a9c51809a55a/datastream/OBJ/view