Print Email Facebook Twitter Endocytosis of nutrient transporters in fungi Title Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking Author Barata-Antunes, Cláudia (University of Minho) Alves, Rosana (University of Minho) Talaia, Gabriel (University of Minho; Yale University) Casal, Margarida (University of Minho) Gerós, Hernâni (University of Minho; Universidade de Trás-os-Montes e Alto Douro) Mans, R. (TU Delft BT/Industriele Microbiologie) Paiva, Sandra (University of Minho) Date 2021 Abstract Plasma membrane transporters play pivotal roles in the import of nutrients, including sugars, amino acids, nucleobases, carboxylic acids, and metal ions, that surround fungal cells. The selective removal of these transporters by endocytosis is one of the most important regulatory mechanisms that ensures a rapid adaptation of cells to the changing environment (e.g., nutrient fluctuations or different stresses). At the heart of this mechanism lies a network of proteins that includes the arrestin‐related trafficking adaptors (ARTs) which link the ubiquitin ligase Rsp5 to nutrient transporters and endocytic factors. Transporter conformational changes, as well as dynamic interactions between its cytosolic termini/loops and with lipids of the plasma membrane, are also critical during the endocytic process. Here, we review the current knowledge and recent findings on the molecular mechanisms involved in nutrient transporter endocytosis, both in the budding yeast Saccharomyces cerevisiae and in some species of the filamentous fungus Aspergillus. We elaborate on the physiological importance of tightly regulated endocytosis for cellular fitness under dynamic conditions found in nature and highlight how further understanding and engineering of this process is essential to maximize titer, rate and yield (TRY)-values of engineered cell factories in industrial biotechnological processes. Subject ArrestinsAspergilliBiotechnologyCell factoriesConformational changesEndocytic signalsEndocytosisFungiMCCs/eisosomesMetabolismNutrient transportersSaccharomyces cerevisiaeSignaling pathwaysStructure-functionUbiquitylation To reference this document use: http://resolver.tudelft.nl/uuid:90992530-8cc8-4ec8-8900-ad09407f2960 DOI https://doi.org/10.1016/j.csbj.2021.03.013 ISSN 2001-0370 Source Computational and Structural Biotechnology Journal, 19, 1713-1737 Part of collection Institutional Repository Document type review Rights © 2021 Cláudia Barata-Antunes, Rosana Alves, Gabriel Talaia, Margarida Casal, Hernâni Gerós, R. Mans, Sandra Paiva Files PDF 1_s2.0_S2001037021000854_main.pdf 5.28 MB Close viewer /islandora/object/uuid:90992530-8cc8-4ec8-8900-ad09407f2960/datastream/OBJ/view