Print Email Facebook Twitter Enantioselective Michael Addition of Water Title Enantioselective Michael Addition of Water Author Chen, B.S. Resch, V. Otten, L.G. Hanefeld, U. Faculty Applied Sciences Department BT/Biotechnology Date 2014-12-21 Abstract The enantioselective Michael addition using water as both nucleophile and solvent has to date proved beyond the ability of synthetic chemists. Herein, the direct, enantioselective Michael addition of water in water to prepare important ?-hydroxy carbonyl compounds using whole cells of Rhodococcus strains is described. Good yields and excellent enantioselectivities were achieved with this method. Deuterium labeling studies demonstrate that a Michael hydratase catalyzes the water addition exclusively with anti-stereochemistry. Subject biocatalysisenantioselectivityhydratasesMichael additionwater To reference this document use: http://resolver.tudelft.nl/uuid:acd6dca6-d32c-4f57-9ec8-db95bb9ba403 DOI https://doi.org/10.1002/chem.201405579 Publisher Wiley ISSN 0947-6539 Source Chemistry: A European journal, 21 (7), 2015 Part of collection Institutional Repository Document type journal article Rights © 2015 The AuthorsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Files PDF Hanefeld_2015.pdf 727.98 KB Close viewer /islandora/object/uuid:acd6dca6-d32c-4f57-9ec8-db95bb9ba403/datastream/OBJ/view