Print Email Facebook Twitter A Miniaturized EHT Platform for Accurate Measurements of Tissue Contractile Properties Title A Miniaturized EHT Platform for Accurate Measurements of Tissue Contractile Properties Author Dostanic, M. (TU Delft Electronic Components, Technology and Materials) Stein, Jeroen (Leiden University Medical Center) Windt, Laura (Leiden University Medical Center) van Meer, Berend (Leiden University Medical Center) Bellin, Milena (Leiden University Medical Center; Università degli Studi di Padova) Orlova, Valeria (Leiden University Medical Center) Mastrangeli, Massimo (TU Delft Electronic Components, Technology and Materials) Mummery, Christine (Leiden University Medical Center) Sarro, Pasqualina M (TU Delft Electronic Components, Technology and Materials) Date 2020-07-30 Abstract We present a wafer-scale fabricated, PDMS-based platform for culturing miniaturized engineered heart tissues (EHTs) which allows highly accurate measurements of the contractile properties of these tissues. The design of the platform is an anisometrically downscaled version of the Heart-Dyno system, consisting of two elastic micropillars inside an elliptic microwell with volume ranging from 3 down to 1μL which supports EHT formation. Size downscaling facilitates fabrication of the platform and makes it compatible with accurate and highly reproducible batch wafer-scale processing; furthermore, downscaling reduces the cost of cell cultures and increases assay throughput. After fabrication, the devices were characterized by nanoindentation to assess the mechanical properties of the pillars and transferred to 96-well plates for cell seeding. Regardless the size of the platform, cell seeding resulted in successful formation of EHTs and all tissues were functionally active (i.e. showed cyclic contractions). The precise characterization of the stiffness of the micropillars enabled accurate measurements of the contractile forces exerted by the cardiac tissues through optical tracking of micropillar displacement. The miniature EHT platforms described in this paper represent a proper microenvironment for culturing and studying EHTs. Subject Engineered heart tissuemicrofabricationnanoindentationorgans-on-chippluripotent stem cells To reference this document use: http://resolver.tudelft.nl/uuid:d9dfd7ba-7a5b-48d1-927f-fb73248b7a93 DOI https://doi.org/10.1109/JMEMS.2020.3011196 ISSN 1057-7157 Source IEEE Journal of Microelectromechanical Systems, 29 (5), 881-887 Part of collection Institutional Repository Document type journal article Rights © 2020 M. Dostanic, Jeroen Stein, Laura Windt, Berend van Meer, Milena Bellin, Valeria Orlova, Massimo Mastrangeli, Christine Mummery, Pasqualina M Sarro Files PDF DostanicEtAl_JMEMS2020.pdf 5.42 MB Close viewer /islandora/object/uuid:d9dfd7ba-7a5b-48d1-927f-fb73248b7a93/datastream/OBJ/view