Print Email Facebook Twitter Immunomodulation of surface biofunctionalized 3D printed porous titanium implants Title Immunomodulation of surface biofunctionalized 3D printed porous titanium implants Author Razzi, F. (TU Delft ChemE/Product and Process Engineering) Fratila-Apachitei, E.L. (TU Delft Biomaterials & Tissue Biomechanics) Fahy, N. (Erasmus MC) Bastiaansen-Jenniskens, Yvonne M. (Erasmus MC) Apachitei, I. (TU Delft Biomaterials & Tissue Biomechanics) Farrell, E. (Erasmus MC) Zadpoor, A.A. (TU Delft Biomaterials & Tissue Biomechanics) Date 2020 Abstract Additive manufacturing (AM) techniques have provided many opportunities for the rational design of porous metallic biomaterials with complex and precisely controlled topologies that give rise to unprecedented combinations of mechanical, physical, and biological properties. These favorable properties can be enhanced by surface biofunctionalization to enable full tissue regeneration and minimize the risk of implant-associated infections (IAIs). There is, however, an increasing need to investigate the immune responses triggered by surface biofunctionalized AM porous metals. Here, we studied the immunomodulatory effects of AM porous titanium (Ti-6Al-4V) printed using selective laser melting and of two additional groups consisting of AM implants surface biofunctionalized using plasma electrolytic oxidation (PEO) with/without silver nanoparticles. The responses of human primary macrophages and human mesenchymal stromal cells (hMSCs) were studied in terms of cell viability, cell morphology and biomarkers of macrophage polarization. Non-treated AM porous titanium triggered a strong pro-inflammatory response in macrophages, albeit combined with signs of anti-inflammatory effects. The PEO treatment of AM porous titanium implants showed a higher potential to induce polarization towards a pro-repair macrophage phenotype. We detected no cytotoxicity against hMSCs in any of the groups. However, the incorporation of silver nanoparticles resulted in strong cytotoxicity against attached macrophages. The results of this study indicate the potential immunomodulatory effects of the AM porous titanium enhanced with PEO treatment, and point towards caution and further research when using silver nanoparticles for preventing IAIs. Subject metal 3D printingplasma electrolytic oxidationimmunomodulationmacrophages To reference this document use: http://resolver.tudelft.nl/uuid:e9283915-f324-40bd-984f-f98de7979b6e DOI https://doi.org/10.1088/1748-605X/ab7763 ISSN 1748-6041 Source Biomedical Materials, 15 (3) Part of collection Institutional Repository Document type journal article Rights © 2020 F. Razzi, E.L. Fratila-Apachitei, N. Fahy, Yvonne M. Bastiaansen-Jenniskens, I. Apachitei, E. Farrell, A.A. Zadpoor Files PDF Razzi_2020_Biomed._Mater. ... 035017.pdf 2.76 MB Close viewer /islandora/object/uuid:e9283915-f324-40bd-984f-f98de7979b6e/datastream/OBJ/view