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Conthe Calvo, M. (author), Wittorf, Lea (author), Kuenen, J.G. (author), Kleerebezem, R. (author), Hallin, Sara (author), van Loosdrecht, Mark C.M. (author)Nitrous oxide (N<sub>2</sub>O) reducing microorganisms may be key in the mitigation of N<sub>2</sub>O emissions from managed ecosystems. However, there is still no clear understanding of the physiological and bioenergetic implications of microorganisms possessing either of the two N<sub>2</sub>O reductase genes (nosZ), clade I and the more...journal article 2018
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Lin, Y. (author), Reino, Clara (author), Carrera, Julián (author), Pérez, Julio (author), van Loosdrecht, Mark C.M. (author)A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different glycoconjugates. Its...journal article 2018
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Sanawar, H. (author), Siddiqui, A. (author), Bucs, S.S. (author), Farhat, N. M. (author), van Loosdrecht, Mark C.M. (author), Kruithof, J. C. (author), Vrouwenvelder, J.S. (author)Biofouling studies addressing biofouling control are mostly executed in short-term studies. It is unclear whether data collected from these experiments are representative for long-term biofouling as occurring in full-scale membrane systems. This study investigated whether short-term biofouling studies accelerated by biodegradable nutrient...journal article 2017
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Siddiqui, A (author), Lehmann, S (author), Haaksman, V.A. (author), Ogier, J. (author), Schellenberg, Carsten (author), van Loosdrecht, Mark C.M. (author), Kruithof, J. C. (author), Vrouwenvelder, J.S. (author)The porosity of spacer-filled feed channels influences the hydrodynamics of spiral-wound membrane systems and impacts the overall performance of the system. Therefore, an exact measurement and a detailed understanding of the impact of the feed channel porosity is required to understand and improve the hydrodynamics of spiral-wound membrane...journal article 2017
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Rubio-Rincón, Francisco (author), Lopez-Vazquez, Carlos (author), Welles, L. (author), van den Brand, Tessa (author), Abbas, B.A. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)The concentration of sulphate present in wastewater can vary from 10 to 500 mg SO<sub>4</sub> <sup>2−</sup>/L. During anaerobic conditions, sulphate is reduced to sulphide by sulphate-reducing bacteria (SRB). Sulphide generation is undesired in wastewater treatment plants (WWTPs). Previous research indicated that SRB are inhibited by the...journal article 2017
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Rubio Rincon, F.J. (author), Welles, L. (author), Lopez Vazquez, Carlos (author), Nierychlo, M. (author), Abbas, B.A. (author), Geleijnse, M.A.A. (author), Nielsen, P.H. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)Thiothrix caldifontis was the dominant microorganism (with an estimated bio-volume of 65 ± 3%) in a lab-scale enhanced biological phosphorus removal (EBPR) system containing 100 mg of sulphide per litre in the influent. After a gradual exposure to the presence of sulphide, the EBPR system initially dominated by Candidatus Accumulibacter...journal article 2017
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Pronk, M. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)A new acid soluble extracellular polymeric substance (acid soluble EPS) was extracted from an acetate fed aerobic granular sludge reactor operated at 35 °C. Acid soluble EPS dominated granules exhibited a remarkable and distinctive tangled tubular morphology. These granules are dominated by Defluviicoccus Cluster II organisms. Acetic acid...journal article 2017
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Sorokin, Dimitry Y. (author), Makarova, Kira S. (author), Abbas, B.A. (author), Ferrer, Manuel (author), Golyshin, Peter N. (author), Galinski, Erwin A. (author), Ciordia, Sergio (author), Mena, María Carmen (author), Merkel, Alexander Y. (author), Wolf, Yuri I. (author), van Loosdrecht, Mark C.M. (author), Koonin, Eugene V. (author)Methanogenic archaea are major players in the global carbon cycle and in the biotechnology of anaerobic digestion. The phylum Euryarchaeota includes diverse groups of methanogens that are interspersed with non-methanogenic lineages. So far, methanogens inhabiting hypersaline environments have been identified only within the order...journal article 2017
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Debeljak, Pavla (author), Pinto, M.J. (author), Proietti, Maira (author), Reisser, Julia (author), Ferrari, Francesco F. (author), Abbas, B.A. (author), van Loosdrecht, Mark C.M. (author), Slat, Boyan (author), Herndl, Gerhard J. (author)The ubiquity of plastics in oceans worldwide raises concerns about their ecological implications. Suspended microplastics (<5 mm) can be ingested by a wide range of marine organisms and may accumulate up the food web along with associated chemicals. Additionally, plastics provide a stable substrate to a wide range of organisms and, owing...journal article 2017
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Welles, L. (author), Abbas, B.A. (author), Sorokin, Dimitry Y. (author), Lopez Vazquez, C.M. (author), Hooijmans, C. M. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)The objective of this study was to investigate the ability of a culture highly enriched with the polyphosphate-accumulating organism, “Candidatus Accumulibacter phosphatis” clade IIC, to adjust their metabolism to different phosphate availabilities. For this purpose the biomass was cultivated in a sequencing batch reactor with acetate and...journal article 2017
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Velasco Alvarez, M.I. (author), ten Pierick, A. (author), van Dam, P.T.N. (author), Maleki Seifar, R. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Poly(3-hydroxybutyrate) (PHB) is an interesting biopolymer for replacing petroleum-based plastics, its biological production is performed in natural and engineered microorganisms. Current metabolic engineering approaches rely on high-throughput strain construction and screening. Analytical procedures have to be compatible with the small scale...journal article 2017
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van den Berg, E.M. (author), Perdigão Elisiário, M. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Denitrification and dissimilatory reduction to ammonium (DNRA) are competing nitrate-reduction processes that entail important biogeochemical consequences for nitrogen retention/removal in natural and man-made ecosystems. The nature of the available carbon source and electron donor have been suggested to play an important role on the outcome of...journal article 2017
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van den Berg, E.M. (author), Rombouts, J.L. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes that compete for oxidized nitrogen compounds in the environment. The objective of this work was to determine the role of nitrite versus nitrate as terminal electron acceptor on the competition between DNRA and denitrification. Initially, a mixed...journal article 2017
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Hoekstra, M. (author), de Weerd, F.A. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)The implementation of autotrophic nitrogen removal in the mainstream of a municipal wastewater treatment plant is currently pursued. Among the crucial unknown factors are the kinetic properties of anaerobic ammonium oxidising (anammox) bacteria at low temperatures. In this study we investigated the adaptation of a fast-growing anammox culture...journal article 2017
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Rubio Rincon, F.J. (author), Lopez-Vazquez, C. M. (author), Welles, L. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)Although simultaneous P-removal and nitrate reduction has been observed in laboratory studies as well as full-scale plants, there are contradictory reports on the ability of PAO I to efficiently use nitrate as electron acceptor. Such discrepancy could be due to other microbial groups performing partial denitrification from nitrate to nitrite....journal article 2017
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Suresh Kumar, P. (author), Prot, T.J.F. (author), Korving, Leon (author), Keesman, Karel J. (author), Dugulan, A.I. (author), van Loosdrecht, Mark C.M. (author), Witkamp, G.J. (author)Adsorption is often suggested for to reach very low phosphate levels in municipal wastewater effluent and even to recover phosphate. Adsorbent performance is usually associated with surface area but the exact role of the pore size distribution (PSD) is unclear. Here, we show the effect of the PSD on phosphate adsorption. Granular activated...journal article 2017
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van den Berg, E.M. (author), Boleij, M. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) compete for nitrate in natural and engineered environments. A known important factor in this microbial competition is the ratio of available electron donor and elector acceptor, here expressed as Ac/N ratio (acetate/nitrate-nitrogen). We studied the impact of the Ac/N ratio...journal article 2016
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Wilfert, P.K. (author), Mandalidis, A. (author), Dugulan, A.I. (author), Goubitz, K. (author), Korving, L. (author), Temmink, H. (author), Witkamp, G.J. (author), van Loosdrecht, Mark C.M. (author)Iron is an important element for modern sewage treatment, inter alia to remove phosphorus from sewage. However, phosphorus recovery from iron phosphorus containing sewage sludge, without incineration, is not yet economical. We believe, increasing the knowledge about iron-phosphorus speciation in sewage sludge can help to identify new routes...journal article 2016
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Prest, E.I.E.D. (author), Weissbrodt, D.G. (author), Hammes, F (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Large seasonal variations in microbial drinking water quality can occur in distribution networks, but are often not taken into account when evaluating results from short-term water sampling campaigns. Temporal dynamics in bacterial community characteristics were investigated during a two-year drinking water monitoring campaign in a full-scale...journal article 2016
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Felz, S. (author), Al-Zuhairy, S.H.K. (author), Aarstad, Olav Andreas (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)To evaluate and develop methodologies for the extraction of gel-forming extracellular polymeric substances (EPS), EPS from aerobic granular sludge (AGS) was extracted using six different methods (centrifugation, sonication, ethylenediaminetetraacetic acid (EDTA), formamide with sodium hydroxide (NaOH), formaldehyde with NaOH and sodium...journal article 2016