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Hoekstra, M. (author), Geilvoet, Stefan P. (author), Hendrickx, Tim L.G. (author), van Erp Taalman Kip, Charlotte S. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)The aim of this research was to study the biological feasibility of the Partial Nitritation/Anammox (PN/A) technology to remove nitrogen from municipal mainstream wastewaters. During stable process operations at summer temperatures (23.2 ± 1.3°C), the total nitrogen removal rate was 0.223 ± 0.029 kg N (m<sup>3 </sup>d)<sup>−1</sup> while at...journal article 2018
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Peters, M.C.F.M. (author), Keuten, M.G.A. (author), Knezev, Aleksandra (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author), Rietveld, L.C. (author), de Kreuk, M.K. (author)Bathers release bacteria in swimming pool water, but little is known about the fate of these bacteria and potential risks they might cause. Therefore, shower water was characterized and subjected to chlorination to identify the more chlorine-resistant bacteria that might survive in a chlorinated swimming pool and therefore could form a...journal article 2018
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El Chakhtoura, J. (author), Saikaly, Pascal E. (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)We sampled the tap water of seven unique, full-scale drinking water distribution systems at different locations as well as the corresponding treatment plant effluents to evaluate the impact of distribution and the potential presence of a core drinking water microbiome. The water was also sampled during network flushing to examine its effect on...journal article 2018
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de Graaff, D.R. (author), van Dijk, E.J.H. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)For a stable operation, the aerobic granular sludge process requires mechanically strong granules in balance with the shear forces in the reactor. Despite a wide general interest in granular stability, the mechanical strength of both anaerobic and aerobic granular sludge received very little attention. In this study, a high-shear method for...journal article 2018
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van den Brand, Tessa (author), Snip, Laura (author), Palmen, Luc (author), Weij, Paul (author), Sipma, Jan (author), van Loosdrecht, Mark C.M. (author)The application of sulfate reducing bacteria (SRB) to treat municipal wastewater is seldom considered. For instance, due to low sludge yield it can reduce the amount of excess sludge produced significantly. Several studies, mainly at laboratory-scale, revealed that SRB can proliferate in artificial wastewater systems at temperatures of 20°C...journal article 2018
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Boleij, M. (author), Pabst, Martin (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules. To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS...journal article 2018
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Suresh Kumar, P. (author), Ejerssa, Wondesen Workneh (author), Wegener, Carita Clarissa (author), Korving, Leon (author), Dugulan, A.I. (author), Temmink, Hardy (author), van Loosdrecht, Mark C.M. (author), Witkamp, G.J. (author)Phosphate is a vital nutrient for life but its discharge from wastewater effluents can lead to eutrophication. Adsorption can be used as effluent polishing step to reduce phosphate to very low concentrations. Adsorbent reusability is an important parameter to make the adsorption process economically feasible. This implies that the adsorbent...journal article 2018
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Jafari, Morez (author), Desmond, Peter (author), van Loosdrecht, Mark C.M. (author), Derlon, Nicolas (author), Morgenroth, Eberhard (author), Picioreanu, C. (author)Biofilm formation in membrane systems negatively impacts the filtration system performances. This study evaluated how biofilm compression driven by permeate flow increases the hydraulic resistance and leads to reduction in permeate flux. We analysed the effect of biofilm compression on hydraulic resistance and permeate flux through...journal article 2018
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Mannina, Giorgio (author), Chandran, Kartik (author), Capodici, Marco (author), Cosenza, Alida (author), Di Trapani, Daniele (author), van Loosdrecht, Mark C.M. (author)This study aimed at evaluating the nitrous oxide (N2O) emissions from membrane bioreactors (MBRs) for wastewater treatment. The study investigated the N2O emissions considering multiple influential factors over a two-year period: (i) different MBR based process configurations; (ii) wastewater composition (municipal or industrial); (iii)...journal article 2018
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Sanawar, H. (author), Pinel, I.S.M. (author), Farhat, N. M. (author), Bucs, Sz S. (author), Zlopasa, J. (author), Kruithof, J. C. (author), Witkamp, G.J. (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Chemical cleaning is routinely performed in reverse osmosis (RO) plants for the regeneration of RO membranes that suffer from biofouling problems. The potential of urea as a chaotropic agent to enhance the solubilization of biofilm proteins has been reported briefly in the literature. In this paper the efficiency of urea cleaning for RO...journal article 2018
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Conthe Calvo, M. (author), Wittorf, Lea (author), Kuenen, J.G. (author), Kleerebezem, R. (author), Hallin, Sara (author), van Loosdrecht, Mark C.M. (author)Nitrous oxide (N<sub>2</sub>O) reducing microorganisms may be key in the mitigation of N<sub>2</sub>O emissions from managed ecosystems. However, there is still no clear understanding of the physiological and bioenergetic implications of microorganisms possessing either of the two N<sub>2</sub>O reductase genes (nosZ), clade I and the more...journal article 2018
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Lin, Y. (author), Reino, Clara (author), Carrera, Julián (author), Pérez, Julio (author), van Loosdrecht, Mark C.M. (author)A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different glycoconjugates. Its...journal article 2018
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Sanawar, H. (author), Siddiqui, A. (author), Bucs, S.S. (author), Farhat, N. M. (author), van Loosdrecht, Mark C.M. (author), Kruithof, J. C. (author), Vrouwenvelder, J.S. (author)Biofouling studies addressing biofouling control are mostly executed in short-term studies. It is unclear whether data collected from these experiments are representative for long-term biofouling as occurring in full-scale membrane systems. This study investigated whether short-term biofouling studies accelerated by biodegradable nutrient...journal article 2017
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Siddiqui, A (author), Lehmann, S (author), Haaksman, V.A. (author), Ogier, J. (author), Schellenberg, Carsten (author), van Loosdrecht, Mark C.M. (author), Kruithof, J. C. (author), Vrouwenvelder, J.S. (author)The porosity of spacer-filled feed channels influences the hydrodynamics of spiral-wound membrane systems and impacts the overall performance of the system. Therefore, an exact measurement and a detailed understanding of the impact of the feed channel porosity is required to understand and improve the hydrodynamics of spiral-wound membrane...journal article 2017
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Rubio-Rincón, Francisco (author), Lopez-Vazquez, Carlos (author), Welles, L. (author), van den Brand, Tessa (author), Abbas, B.A. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)The concentration of sulphate present in wastewater can vary from 10 to 500 mg SO<sub>4</sub> <sup>2−</sup>/L. During anaerobic conditions, sulphate is reduced to sulphide by sulphate-reducing bacteria (SRB). Sulphide generation is undesired in wastewater treatment plants (WWTPs). Previous research indicated that SRB are inhibited by the...journal article 2017
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Rubio Rincon, F.J. (author), Welles, L. (author), Lopez Vazquez, Carlos (author), Nierychlo, M. (author), Abbas, B.A. (author), Geleijnse, M.A.A. (author), Nielsen, P.H. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)Thiothrix caldifontis was the dominant microorganism (with an estimated bio-volume of 65 ± 3%) in a lab-scale enhanced biological phosphorus removal (EBPR) system containing 100 mg of sulphide per litre in the influent. After a gradual exposure to the presence of sulphide, the EBPR system initially dominated by Candidatus Accumulibacter...journal article 2017
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Pronk, M. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)A new acid soluble extracellular polymeric substance (acid soluble EPS) was extracted from an acetate fed aerobic granular sludge reactor operated at 35 °C. Acid soluble EPS dominated granules exhibited a remarkable and distinctive tangled tubular morphology. These granules are dominated by Defluviicoccus Cluster II organisms. Acetic acid...journal article 2017
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Sorokin, Dimitry Y. (author), Makarova, Kira S. (author), Abbas, B.A. (author), Ferrer, Manuel (author), Golyshin, Peter N. (author), Galinski, Erwin A. (author), Ciordia, Sergio (author), Mena, María Carmen (author), Merkel, Alexander Y. (author), Wolf, Yuri I. (author), van Loosdrecht, Mark C.M. (author), Koonin, Eugene V. (author)Methanogenic archaea are major players in the global carbon cycle and in the biotechnology of anaerobic digestion. The phylum Euryarchaeota includes diverse groups of methanogens that are interspersed with non-methanogenic lineages. So far, methanogens inhabiting hypersaline environments have been identified only within the order...journal article 2017
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Verhagen, K.J.A. (author), Gabriel Guedes da Silva, L. (author), van de Wijgaart, R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Natural habitats of microorganisms are dynamic environments with non-continuous supply of carbon and energy sources, in which intermediate storage of substrates can increase competitiveness. Plasticicumulans acidivorans are polyhydroxybutyrate (PHB) accumulating bacteria enriched from activated sludge using carbon feast-famine cycles as...poster 2017
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Debeljak, Pavla (author), Pinto, M.J. (author), Proietti, Maira (author), Reisser, Julia (author), Ferrari, Francesco F. (author), Abbas, B.A. (author), van Loosdrecht, Mark C.M. (author), Slat, Boyan (author), Herndl, Gerhard J. (author)The ubiquity of plastics in oceans worldwide raises concerns about their ecological implications. Suspended microplastics (<5 mm) can be ingested by a wide range of marine organisms and may accumulate up the food web along with associated chemicals. Additionally, plastics provide a stable substrate to a wide range of organisms and, owing...journal article 2017