document

Improving the indoor / outdoor ratio of (ultra)fine particles in a school

Van Dijken, Froukje (author), te Kulve, Marije (author), Ursem, W.N.J. (author)

Buildings located close to busy roads, industry or stock farms, are of risk of increased indoor particle concentrations, which negatively impacts the health of the building occupants. In order to reduce the exposure of the building occupants, it is important to take measures to reduce the concentration of particulate matter indoors. Solutions...

conference paper 2022

document

Cofactor Specificity of the Bifunctional Alcohol and Aldehyde Dehydrogenase (AdhE) in Wild-Type and Mutant Clostridium thermocellum and Thermoanaerobacteriumsaccharolyticum

Zheng, T. (author), Olson, D.G. (author), Tian, L. (author), Bomble, Y.J. (author), Himmel, M.E. (author), Lo, J. (author), Hon, S. (author), Shaw, A.J. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)

Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are thermophilic bacteria that have been engineered to produce ethanol from the cellulose and hemicellulose fractions of biomass, respectively. Although engineered strains of T. saccharolyticum produce ethanol with a yield of 90% of the theoretical maximum, engineered strains of...

journal article 2015

document

The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading

Holwerda, E.K. (author), Thorne, P.G. (author), Olson, D.G. (author), Amador-Noguez, D. (author), Engle, N.L. (author), Tschaplinksi, T.J. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)

Background Clostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concentrations of ?10 g/L. However, industrially relevant concentrations of substrate start at 100 g/L carbohydrate, which...

journal article 2014

document

Atypical Glycolysis in Clostridium thermocellum

Zhou, J. (author), Olson, D.G. (author), Argyros, D.A. (author), Deng, Y. (author), Van Gulik, W.M. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)

Cofactor specificities of glycolytic enzymes in Clostridium thermocellum were studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate (PPi)-linked activity....

journal article 2013

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Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export

Zelle, R.M. (author), De Hulster, E. (author), Van Winden, W.A. (author), De Waard, P. (author), Dijkema, C. (author), Winkler, A.A. (author), Geertman, J.M. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proceeds via carboxylation of...

journal article 2008

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Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of L-Arabinose

Wisselink, H.W. (author), Toirkens, M.J. (author), Del Rosario Franco Berriel, M. (author), Winkler, A.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as L-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the organism used in industrial ethanol production,...

journal article 2007

document

Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component

Van Maris, A.J.A. (author), Winkler, A.A. (author), Kuyper, M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

Metabolic engineering of Saccharomyces cerevisiae for ethanol production from d-xylose, an abundant sugar in plant biomass hydrolysates, has been pursued vigorously for the past 15 years. Whereas wild-type S. cerevisiae cannot ferment d-xylose, the ketoisomer d-xylulose can be metabolised slowly. Conversion of d-xylose into d-xylulose is...

journal article 2007

document

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: Current status

Van Maris, A.J.A. (author), Abbott, D.A. (author), Bellissimi, E. (author), Van den Brink, J. (author), Kuyper, M. (author), Lutik, M.A.H. (author), Wisselink, H.W. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

journal article 2006

document

Metabolic engineering of xylose fermenting eukaryotic cells

Winkler, A.A. (author), Kuyper, S.M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

The present invention relates to further genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers the host cell the ability of isomerising xylose to xylulose. The further genetic modifications are aimed at improving the efficiency of xylose metabolism and include e.g. reduction of...

patent 2006

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Carbonic anhydrase (Nce103p): An essential biosynthetic enzyme for growth of Saccharomyces cerevisiae at atmospheric carbon dioxide pressure

Aguilera, J. (author), Van Dijken, J.P. (author), De Winde, J.H. (author), Pronk, J.T. (author)

journal article 2005

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Metabolic Engineering of Glycerol Production in Saccharomyces cerevisiae

Overkamp, K.M. (author), Bakker, B.M. (author), Kotter, P. (author), Luttik, M.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

journal article 2002

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Effect of Specific Growth Rate on Fermentative Capacity of Baker¿s Yeast

Van Hoek, P. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

journal article 1998

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The Two Acetyl-coenzyme A Synthetases of Saccharomyces cerevisiae Differ with Respect to Kinetic Properties and Transcriptional Regulation

Van den Berg, M.A. (author), De Jong-Gubbels, P. (author), Kortland, C.J. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Steensma, H.Y. (author)

journal article 1996

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Beijerinck Centennial Microbial Physiology and Gene Regulation: Emerging Principles and Applications

Scheffers, W.A. (author), Van Dijken, J.P. (author)

Book of abstracts of Beijerinck Centennial Microbial Physiology and Gene Regulation: Emerging Principles and Applications, 10-14 December 1995 The Hague, The Netherlands. Under the auspices of the Netherlands Foundation for Biotechnology.

book 1995

document

Effects of growth conditions on mitochondrial morphology in Saccharomyces cerevisiae

Visser, W. (author), Van Spronsen, E.A. (author), Nanninga, N. (author), Pronk, J.T. (author), Kuenen, J.G. (author), Van Dijken, J.P. (author)

journal article 1995

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Effects of oxygen limitation on sugar metabolism in yeasts: A continuous-culture study of the Kluyver effect

Weusthuis, R.A. (author), Visser, W. (author), Pronk, J.T. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1994

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Purification and Partial Characterization of Thiosulfate Dehydrogenase from Thiobacillus-Acidophilus

Meulenberg, R. (author), Pronk, J.T. (author), Hazeu, W. (author), Van Dijken, J.P. (author), Frank, J. (author), Bos, P. (author), Kuenen, J.G. (author)

journal article 1993

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Experimental and Theoretical Discrepancies in Growth Yields of Acinetobacter-Calcoaceticus - a Correction of Published Data

Van Schie, B.J. (author), Pronk, J.T. (author), Van Dijken, J.P. (author), Kuenen, J.G. (author)

journal article 1993

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Effect of benzoic acid on metabolic fluxes in yeasts: A continuous-culture study on the regulation of respiration and alcoholic fermentation

Verduyn, C. (author), Postma, E. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1992

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HIGH YIELD METHOD OF GROWING THIOBACILLUS FERROOXIDANS ON FORMATE

Pronk, J.T. (author), Van Dijken, J.P. (author), Bos, P. (author), Kuenen, J.G. (author)

patent 1992