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Hagen, G.M. (author), Caarls, W. (author), Thomas, M. (author), Hill, A. (author), Lidke, K.A. (author), Rieger, B. (author), Fritsch, C. (author), Van Geest, B. (author), Jovin, T.M. (author), Arndt-Jovin, D.J. (author)
We report on a new generation, commercial prototype of a programmable array optical sectioning fluorescence microscope (PAM) for rapid, light efficient 3D imaging of living specimens. The stand-alone module, including light source(s) and detector(s), features an innovative optical design and a ferroelectric liquid-crystal-on-silicon (LCoS)...
conference paper 2007
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Rieger, B. (author), Van Vliet, L.J. (author)
conference paper 2001
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Chakrova, N. (author), Rieger, B. (author), Stallinga, S. (author)
We present a versatile fluorescence microscope, built by complementing a conventional fluorescence microscope with a digital micro-mirror device (DMD) in the illumination path. Arbitrary patterns can be created on the DMD and projected onto the sample. This patterned illumination can be used to improve lateral and axial resolution over the...
conference paper 2015
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Dietrich, H.R.C. (author), Vermolen, B.J. (author), Rieger, B. (author), Young, I.T. (author), Garini, Y. (author)
Single-molecule techniques continue to gain in popularity in research disciplines such as the study of intermolecular interactions. These techniques provide information that otherwise would be lost by using bulk measurements that deal with a large number of molecules. We describe in this report the motion of tethered DNA molecules that have been...
conference paper 2007
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Guo, Y. (author), Rieger, B. (author)
With electron tomography, we can reconstruct a threedimensional<br/>(3D) volume of a specimen from a series of its two-dimensional (2D) projection images on the nanoscale. In a scanning transmission electron microscope (STEM), element-specific maps and mass-contrast projections can be simultaneously acquired from the X-ray spectrometer and...
conference paper 2018
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Guo, Y. (author), Rieger, B. (author)
Bimodal tomography introduces a weighting factor α to incorporate X-ray data into projection images acquired from scanning transmission electron microscope (STEM) for achieving an atom-specific three-dimensional (3D) reconstruction of an object on the nanoscale. Currently its value is chosen by computing reconstructions for a large range of...
conference paper 2018
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Guo, Y. (author), Rieger, B. (author)
Regularization has been introduced to electron tomography for enhancing the reconstruction quality. Since over-regularization smears out sharp edges and under-regularization leaves the image too noisy, finding the optimal regularization strength is crucial. To this end, one can either manually tune regularization parameters by trial and error...
conference paper 2019
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Rieger, B. (author)
In this thesis we investigate the measurement of local properties in multi-dimensional grey-value images. Special attention is given to orientation representation and curvature estimation. Furthermore, the possibility to compute global shape properties from these local properties. We aim to derive sampling-error free measurements. A sampling...
doctoral thesis 2004
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Rieger, V.S. (author)
Emissions of road traffic crucially influence Earth’s climate. The vehicle fleet emits not only carbon dioxide (CO2), but also nitrogen oxides (NOx), volatile organic compounds (VOC) and carbon monoxide (CO) which produce ozone (O3) and destroy methane (CH4) in the troposphere. As the demand of mobility is expected to further increase in future,...
doctoral thesis 2018
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Nieuwenhuizen, R.P.J. (author), Nahidiazar, L. (author), Manders, E.M.M. (author), Jalink, K. (author), Stallinga, S. (author), Rieger, B. (author)
Co-localization analysis is a widely used tool to seek evidence for functional interactions between molecules in different color channels in microscopic images. Here we extend the basic co-localization analysis by including the orientations of the structures on which the molecules reside. We refer to the combination of co-localization of...
journal article 2015
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Rieger, B. (author), van Vliet, L.J. (author)
journal article 2002
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Dietrich, H.R.C. (author), Rieger, B. (author), Wiertz, F.G.M. (author), De Groote, F.H. (author), Heering, H.A. (author), Young, I.T. (author), Garini, Y. (author)
journal article 2009
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Vulovic, M. (author), Rieger, B. (author), Van Vliet, L.J. (author), Koster, A.J. (author), Ravelli, R.B.G. (author)
Charge-coupled devices (CCD) are nowadays commonly utilized in transmission electron microscopy (TEM) for applications in life sciences. Direct access to digitized images has revolutionized the use of electron microscopy, sparking developments such as automated collection of tomographic data, focal series, random conical tilt pairs and...
journal article 2009
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Löschberger, A. (author), Van de Linde, S. (author), Debauvalle, M.C. (author), Rieger, B. (author), Heilemann, M. (author), Krohne, G. (author), Sauer, M. (author)
One of the most complex molecular machines of cells is the nuclear pore complex (NPC), which controls all trafficking of molecules in and out of the nucleus. Because of their importance for cellular processes such as gene expression and cytoskeleton organization, the structure of NPCs has been studied extensively during the last few decades,...
journal article 2012
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Selig, B. (author), Vermeer, K.A. (author), Rieger, B. (author), Hillenaar, T. (author), Hendriks, C.L.L. (author)
Background Manual and semi-automatic analyses of images, acquired in vivo by confocal microscopy, are often used to determine the quality of corneal endothelium in the human eye. These procedures are highly time consuming. Here, we present two fully automatic methods to analyze and quantify corneal endothelium imaged by in vivo white light slit...
journal article 2015
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Nieuwenhuizen, R.P.J. (author), Bates, M. (author), Szymborska, A. (author), Lidke, K.A. (author), Rieger, B. (author), Stallinga, S. (author)
Quantification in localization microscopy with reversibly switchable fluorophores is severely hampered by the unknown number of switching cycles a fluorophore undergoes and the unknown stoichiometry of fluorophores on a marker such as an antibody. We overcome this problem by measuring the average number of localizations per fluorophore, or...
journal article 2015
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Meddens, M.B.M. (author), Rieger, B. (author), Figdor, C.G. (author), Cambi, A. (author), Van den Dries, K. (author)
Podosomes are cellular adhesion structures involved in matrix degradation and invasion that comprise an actin core and a ring of cytoskeletal adaptor proteins. They are most often identified by staining with phalloidin, which binds F-actin and therefore visualizes the core. However, not only podosomes, but also many other cytoskeletal structures...
journal article 2013
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Brinkers, S. (author), Dietrich, H.R.C. (author), De Groote, F.H. (author), Young, I.T. (author), Rieger, B. (author)
The wormlike chain model describes the micromechanics of semiflexible polymers by introducing the persistence length. We propose a method of measuring the persistence length of DNA in a controllable near-native environment. Using a dark field microscope, the projected positions of a gold nanoparticle undergoing constrained Brownian motion are...
journal article 2009
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Rieger, B. (author), van den Doel, L.R. (author), van Vliet, L.J. (author)
journal article 2003
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Voortman, L.M. (author), Stallinga, S. (author), Schoenmakers, R.H.M. (author), Van Vliet, L.J. (author), Rieger, B. (author)
Today, the resolution in phase-contrast cryo-electron tomography is for a significant part limited by the contrast transfer function (CTF) of the microscope. The CTF is a function of defocus and thus varies spatially as a result of the tilting of the specimen and the finite specimen thickness. Models that include spatial dependencies have not...
journal article 2011
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