Precision Engineering of the Co-immobilization of Enzymes for Cascade Biocatalysis

The design and orderly layered co-immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the...

A novel 4H-SiC multiple stepped SGT MOSFET with improved high frequency figure of merit

A novel 4H-SiC Multiple Stepped SGT MOSFET (MSGT-MOSFET) is presented and investigated utilizing TCAD simulations in this paper. We have quantitatively studied the characteristics of the device through simulation modeling and physical model calculations, and comparatively analyzed the performance and application prospects of this novel device...

Laser-Induced Graphene Formation on Chitosan Derivatives toward Ecofriendly Electronics

Laser-induced graphene (LIG) has aroused a wide range of research interests ranging from micro-nano energy devices to the Internet of Things (IoT). Nevertheless, the non-degradability of most-used synthetic polymer carbon sources poses a serious threat to the environment. In this work, ecofriendly chitosan-based derivatives, including...

Rapid extraction of pavement aggregate gradation based on point clouds using deep learning networks

Usage of asphalt mixture with poor gradation will most likely lead to pavement deficiency. There is a growing need for rapid and non-destructive methods to extract pavement aggregate gradation. In this study, a deep learning-based method that utilizes point clouds data for gradation extraction was proposed. Firstly, a data enhancement...

Designing an enzyme assembly line for green cascade processes using bio-orthogonal chemistry

Two non-canonical amino acids (ncAAs) with bio-orthogonal reactive groups, namely, p-azido-l-phenylalanine (p-AzF) and p-propargyloxy-l-phenylalanine (p-PaF), were genetically inserted into an aldo-keto reductase (AKR) and an alcohol dehydrogenase (ADH), respectively, at two preselected sites for each enzyme. The variants were expressed in...

Air entrainment and free-surface fluctuations in A-type hydraulic jumps with an abrupt drop

In high dam construction projects in China, stilling basin design with an abrupt bottom drop is sometimes introduced to reduce the bottom velocity and pressure loads by generating A-type hydraulic jumps. Although the stilling basin design is not new, A-type hydraulic jumps have not been studied taking into account the air entrainment and...

Engineering ketoreductases for the enantioselective synthesis of chiral alcohols

The use of engineered ketoreductases (KREDS), both as whole microbial cells and isolated enzymes, in the highly enantiospecific reduction of prochiral ketones is reviewed. The homochiral alcohol products are key intermediates in, for example, pharmaceuticals synthesis. The application of sophisticated protein engineering and enzyme...

Towards weathering and corrosion resistant, self-warning and self-healing epoxy coatings with tannic acid loaded nanocontainers

In this work, an active protective epoxy coating with weathering resistant, corrosion-warning, and self-healing properties was developed by incorporating tannic acid (TA) loaded mesoporous silica (MSN-TA) nanocontainers. The introduction of MSN-TA nanocontainers could alleviate the coating degradation via scavenging the radicals generated...

Investigation of Unclamped Inductive Switch Characteristics in 4H-SiC MOSFETs With Different Cell Topologies

To investigate the unclamped inductive switch (UIS) characteristics, 1200 V silicon carbide (SiC) planar MOSFETs with four cell topologies of linear, current sharing linear, square, and hexagon are designed and manufactured. The experimental platform was built and tested. The results show that the single pulse avalanche energy density of the...

Lateral variation in slab window viscosity inferred from global navigation satellite system (GNSS)–observed uplift due to recent mass loss at Patagonia ice fields

The geographic coincidence of the Chile Ridge slab window and the Patagonia ice fields offers a unique opportunity for assessing the effects of slab window rheology on glacial isostatic adjustment (GIA). Mass loss of these ice fields since the Little Ice Age causes rapid but variable crustal uplift, 12–24 mm/yr around the North Patagonia ice...

A numerical assessment on the managed aquifer recharge to achieve sustainable groundwater development in Chaobai River area, Beijing, China

Intensive groundwater exploitation has depleted groundwater storage and led to a series of geo-environmental problems in Beijing Plain, China. Managed Aquifer Recharge (MAR) has been endorsed to mitigate the groundwater storage depletion and achieve groundwater sustainability. A pilot MAR has been tested in the Chaobai River catchment since...

Ensemble of Metaheuristic and Exact Algorithm Based on the Divide-And-Conquer Framework for Multisatellite Observation Scheduling

Satellite observation scheduling plays a significant role in improving the efficiency of Earth observation systems. To solve the large-scale multisatellite observation scheduling problem, this article proposes an ensemble of metaheuristic and exact algorithms based on a divide-And-conquer framework (EHE-DCF), including a task allocation phase...

Solvent-Free Three-Dimensional Printing of Biodegradable Elastomers Using Liquid Macrophotoinitiators

Vat photopolymerization 3D printing provides new opportunities for the fabrication of tissue scaffolds and medical devices. However, for the manufacturing of biodegradable elastomers, it usually requires the use of organic solvents to dissolve the solid photoinitators and achieve low resin viscosity, making this process environmentally...

Cascade CO₂ electroreduction enables efficient carbonate-free production of ethylene

CO ₂ electroreduction offers a route to net-zero-emission production of C ₂H ₄—the most-produced organic compound. However, the formation of carbonate in this process causes loss of CO ₂ and a severe energy consumption/production penalty. Dividing the CO ₂-to-C ₂H ₄...

Microwave roasting of blast furnace slag for carbon dioxide mineralization and energy analysis

For both the waste treatment of large quantities of blast furnace (BF) slag and carbon dioxide (CO₂) that are discharged in ironworks, mineral carbonation by BF slag was proposed in this decade. However, it has not been widely used due to its high energy consumption and low production efficiency. In this study, a microwave roasting...

Identification of driving factors for green building development in China

Green building (GB) has been actively promoted in many countries, but it has not become the mainstream in Chinese construction industry due to various reasons. This paper aims to investigate the major driving factors for the development of GB with reference of the Chinese construction market. Twenty-one factors influencing the development of...

Dielectric behavior and phase transition in perovskite oxide Pb(Fe1/2Nb1/2)1?xTixO3 single crystal

Perovskite oxide Pb(Fe1/2Nb1/2)0.52Ti0.48O3 crystals, which were grown using a modified Bridgman method, show a high low-frequency dielectric constant response at room temperature. The Curie phase transition occurs at 523 K. The characteristic frequency of the dielectric dispersion versus the inverse of temperature obeys an Arrhenius relation,...