Condensin extrudes DNA loops in steps up to hundreds of base pairs that are generated by ATP binding events

The condensin SMC protein complex organizes chromosomal structure by extruding loops of DNA. Its ATP-dependent motor mechanism remains unclear but likely involves steps associated with large conformational changes within the ∼50 nm protein complex. Here, using high-resolution magnetic tweezers, we resolve single steps in the loop extrusion...

SMC complexes can traverse physical roadblocks bigger than their ring size

Ring-shaped structural maintenance of chromosomes (SMC) complexes like condensin and cohesin extrude loops of DNA. It remains, however, unclear how they can extrude DNA loops in chromatin that is bound with proteins. Here, we use in vitro single-molecule visualization to show that nucleosomes, RNA polymerase, and dCas9 pose virtually no...

Bridging-induced phase separation induced by cohesin SMC protein complexes

Structural maintenance of chromosome (SMC) protein complexes are able to extrude DNA loops. While loop extrusion constitutes a fundamental building block of chromosomes, other factors may be equally important. Here, we show that yeast cohesin exhibits pronounced clustering on DNA, with all the hallmarks of biomolecular condensation. DNA...

Publisher Correction: The condensin holocomplex cycles dynamically between open and collapsed states (Nature Structural & Molecular Biology, (2020), 27, 12, (1134-1141), 10.1038/s41594-020-0508-3)

An amendment to this paper has been published and can be accessed via a link at the top of the paper.