Precision Engineering of the Co-immobilization of Enzymes for Cascade Biocatalysis

The design and orderly layered co-immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the...

A novel 4H-SiC multiple stepped SGT MOSFET with improved high frequency figure of merit

A novel 4H-SiC Multiple Stepped SGT MOSFET (MSGT-MOSFET) is presented and investigated utilizing TCAD simulations in this paper. We have quantitatively studied the characteristics of the device through simulation modeling and physical model calculations, and comparatively analyzed the performance and application prospects of this novel device...

Rapid extraction of pavement aggregate gradation based on point clouds using deep learning networks

Usage of asphalt mixture with poor gradation will most likely lead to pavement deficiency. There is a growing need for rapid and non-destructive methods to extract pavement aggregate gradation. In this study, a deep learning-based method that utilizes point clouds data for gradation extraction was proposed. Firstly, a data enhancement...

Designing an enzyme assembly line for green cascade processes using bio-orthogonal chemistry

Two non-canonical amino acids (ncAAs) with bio-orthogonal reactive groups, namely, p-azido-l-phenylalanine (p-AzF) and p-propargyloxy-l-phenylalanine (p-PaF), were genetically inserted into an aldo-keto reductase (AKR) and an alcohol dehydrogenase (ADH), respectively, at two preselected sites for each enzyme. The variants were expressed in...

Engineering ketoreductases for the enantioselective synthesis of chiral alcohols

The use of engineered ketoreductases (KREDS), both as whole microbial cells and isolated enzymes, in the highly enantiospecific reduction of prochiral ketones is reviewed. The homochiral alcohol products are key intermediates in, for example, pharmaceuticals synthesis. The application of sophisticated protein engineering and enzyme...

Investigation of Unclamped Inductive Switch Characteristics in 4H-SiC MOSFETs With Different Cell Topologies

To investigate the unclamped inductive switch (UIS) characteristics, 1200 V silicon carbide (SiC) planar MOSFETs with four cell topologies of linear, current sharing linear, square, and hexagon are designed and manufactured. The experimental platform was built and tested. The results show that the single pulse avalanche energy density of the...

Blockchain technologies empowering peer-to-peer trading in multi-energy systems: From advanced technologies towards applications

A Crystal-Less Clock Generation Technique for Battery-Free Wireless Systems

The size of wireless systems is required to be reduced in many applications, such as ultra-low-power sensor nodes and wearable/implantable devices, where battery and crystal are the two main bottlenecks in system miniaturization. In recent years, battery-free radios based on wireless power transfer (WPT) have shown great potential in...

A Series of Ternary Metal Chloride Superionic Conductors for High-Performance All-Solid-State Lithium Batteries

Understanding the relationship between structure, ionic conductivity, and synthesis is the key to the development of superionic conductors. Here, a series of Li_3-3_xM₁₊_xCl₆ (−0.14 < x ≤ 0.5, M = Tb, Dy, Ho, Y, Er, Tm) solid electrolytes with orthorhombic and trigonal structures are...

A 2m-Range 711uW Body Channel Communication Transceiver Featuring Dynamically-Sampling Bias-Free Interface Front End

Body Channel Communication (BCC) offers a low-loss signal transmission medium for ultra-low-power wearable devices on human body [1]. However, the effective communication range on human body is limited to less than 1m in the state-of-the-art BCC transceivers [2], where the signal loss at the interface of body surface and BCC receiver remains to...

Ensemble of Metaheuristic and Exact Algorithm Based on the Divide-And-Conquer Framework for Multisatellite Observation Scheduling

Satellite observation scheduling plays a significant role in improving the efficiency of Earth observation systems. To solve the large-scale multisatellite observation scheduling problem, this article proposes an ensemble of metaheuristic and exact algorithms based on a divide-And-conquer framework (EHE-DCF), including a task allocation phase...

Green Approach for Post-industrial Urban Regeneration: A case of Economic and Technical Development Zone, China

For cities facing post-industrial transition, urban greenways are proven strategies to activate urban spaces and improve the sustainability of cities. However, significant challenges remain when seeking to link greenways as a greenway network and integrate greenway networks to urban spaces. Given this shortcoming, Yantai National Economic and...

Microwave roasting of blast furnace slag for carbon dioxide mineralization and energy analysis

For both the waste treatment of large quantities of blast furnace (BF) slag and carbon dioxide (CO₂) that are discharged in ironworks, mineral carbonation by BF slag was proposed in this decade. However, it has not been widely used due to its high energy consumption and low production efficiency. In this study, a microwave roasting...

Form-finding and construction of ice composite shell structures

By using inflatable moulds and then spraying cellulose-water mixture, one ice dome and two ice towers were built in Harbin in December 2016. During the whole process, form-finding of the inflatable moulds as well as the construction of these ice composite shell structures are very important for the final results.<br/>The mould for the ice dome...

Determination of the intrinsic α/γ interface mobility during massive transformations in interstitial free Fe-X alloys

Kinetics of the austenite (γ) to ferrite (α) transformation and the reverse ferrite (α) to austenite (γ) transformation in a series of Fe-X (X = Ni, Mn and Co) binary alloys has been experimentally and theoretically investigated. A transition from partitioning to partitionless transformation has been predicted to occur during both the γ→α and...