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Samylina, Olga S. (author), Kosyakova, Anastasia I. (author), Krylov, Artem A. (author), Sorokin, Dimitry Y. (author), Pimenov, Nikolay V. (author)A variety of lakes located in the dry steppe area of southwestern Siberia are exposed to rapid climatic changes, including intra-century cycles with alternating dry and wet phases driven by solar activity. As a result, the salt lakes of that region experience significant fluctuations in water level and salinity, which have an essential impact...journal article 2024
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Bale, Nicol J. (author), Sinnighe Damsté, Jaap S. (author), Kublanov, Ilya V. (author)Beta-mannans are insoluble plant polysaccharides with beta-1,4-linked mannose as the backbone. We used three forms of this polysaccharide, namely, pure mannan, glucomannan, and galactomannan, to enrich haloarchaea, which have the ability to utilize mannans for growth. Four mannan-utilizing strains obtained in pure cultures were closely related...journal article 2024
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Khomyakova, Maria A. (author), Merkel, Alexander Y. (author), Slobodkin, Alexander I. (author), Sorokin, Dimitry Y. (author)Highly purified cultures of alkaliphilic aceticlastic methanogens were collected for the first time using methanogenic enrichments with acetate from a soda lake and a terrestrial mud volcano. The cells of two strains were non-motile rods forming filaments. The mud volcano strain M04Ac was alkalitolerant, with the pH range for growth from 7.5 to...journal article 2023
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Elcheninov, Alexander G. (author), Ugolkov, Yaroslav A. (author), Elizarov, Ivan M. (author), Klyukina, Alexandra A. (author), Kublanov, Ilya V. (author), Sorokin, Dimitry Y. (author)Extremely halophilic archaea are one of the principal microbial community components in hypersaline environments. The majority of cultivated haloarchaea are aerobic heterotrophs using peptides or simple sugars as carbon and energy sources. At the same time, a number of novel metabolic capacities of these extremophiles were discovered recently...journal article 2023
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Ahn, Anne Catherine (author), Schuurmans, J. Merijn (author), Sorokin, Dimitry Y. (author), Muyzer, Gerard (author)Haloalkaliphilic chemolithoautotrophic sulfur-oxidizing bacteria belonging to the genus Thioalkalivibrio are highly abundant in microbial communities found in soda lakes and dominant in full-scale bioreactors removing sulfide from industrial waste gases. Despite certain soda lakes being remote and unaffected by anthropogenic activities,...journal article 2023
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Koziaeva, Veronika V. (author), Sorokin, Dimitry Y. (author), Kolganova, Tatiana V. (author), Grouzdev, Denis S. (author)A spiral-shaped, highly motile bacterium was isolated from freshwater sulfidic sediment. Strain J10 <sup>T</sup> is a facultative autotroph utilizing sulfide, thiosulfate, and sulfur as the electron donors in microoxic conditions. Despite high 16S rRNA gene sequence sequence identity to Magnetospirillum gryphiswaldense MSR-1 <sup>T</sup> (99...journal article 2023
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Merkel, Alexander Y. (author), Bale, Nocole J. (author), Sininghe-Damste, Jaap S. (author), Kublanov, Ilya V. (author)Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according to<br/>phylogenomic analysis, and represent a distant novel species group in the genus Halapricum of the family Haloarculaceae....journal article 2023
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Sorokin, Dimitry Y. (author), Tikhonova, Tamara V. (author), Koch, Hanna (author), van den Berg, E.M. (author), Hinderks, Renske S. (author), Pabst, Martin (author), Kuenen, J.G. (author), van Loosdrecht, Mark C.M. (author), Lücker, Sebastian (author)Dissimilatory nitrate reduction to ammonia (DNRA) is a common biochemical process in the nitrogen cycle in natural and man-made habitats, but its significance in wastewater treatment plants is not well understood. Several ammonifying Trichlorobacter strains (former Geobacter) were previously enriched from activated sludge in nitrate-limited...journal article 2023
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La Cono, Violetta (author), Messina, Enzo (author), Reva, Oleg (author), Smedile, Francesco (author), La Spada, Gina (author), Crisafi, Francesca (author), Marturano, Laura (author), Miguez, Noa (author), Sorokin, Dimitry Y. (author)Climate change, desertification, salinisation of soils and the changing hydrology of the Earth are creating or modifying microbial habitats at all scales including the oceans, saline groundwaters and brine lakes. In environments that are saline or hypersaline, the biodegradation of recalcitrant plant and animal polysaccharides can be...journal article 2023
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de Jong, S.I. (author), Sorokin, Dimitry Y. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author), McMillan, D.G.G. (author)Proteomics has greatly advanced the understanding of the cellular biochemistry of microorganisms. The thermoalkaliphile Caldalkalibacillus thermarum TA2.A1 is an organism of interest for studies into how alkaliphiles adapt to their extreme lifestyles, as it can grow from pH 7.5 to pH 11. Within most classes of microbes, the membrane-bound...journal article 2023
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Merkel, Alexander Y. (author), Bale, Nicole J. (author), Sininghe-Damste, Jaap (author), Kublanov, Ilya V. (author)The authors regret that there is a mistake in the strain collection number: instead of UQ 51487 it should be UQM 51487 in the protologue Table 3 of their paper. The corrected protologue Table is presented below.journal article 2023
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Zhilina, Tatjana N. (author), Sorokin, Dimitry Y. (author), Toschakov, Stepan V. (author), Kublanov, Ilya V. (author), Zavarzina, Daria G. (author)Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702 <sup>T</sup> is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum...journal article 2023
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Sorokin, Dimitry Y. (author), Merkel, Alexander Y. (author), Messina, Enzo (author), Tugui, G. (author), Pabst, Martin (author), Golyshin, Peter N. (author), Yakimov, Michail M. (author)Anaerobic carboxydotrophy is a widespread catabolic trait in bacteria, with two dominant pathways: hydrogenogenic and acetogenic. The marginal mode by direct oxidation to CO<sub>2</sub> using an external e-acceptor has only a few examples. Use of sulfidic sediments from two types of hypersaline lakes in anaerobic enrichments with CO as an e...journal article 2022
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de Rink, Rieks (author), Lavender, Micaela (author), Liu, Dandan (author), Klok, Johannes B.M. (author), Sorokin, Dimitry Y. (author), ter Heijne, Annemiek (author), Buisman, Cees J.M. (author)Sulfide oxidizing bacteria (SOB) are widely applied in industry to convert toxic H2S into elemental sulfur. Haloalkaliphilic planktonic SOB can remove sulfide from solution under anaerobic conditions (SOB are ‘charged’), and release electrons at an electrode (discharge of SOB). The effect of this electron shuttling on product formation and...journal article 2022
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Sorokin, Dimitry Y. (author), Merkel, Alexander Y. (author), Abbas, B.A. (author)Methanonatronarchaeia represents a deep-branching phylogenetic lineage of extremely halo(alkali)philic and moderately thermophilic methyl-reducing methanogens belonging to the phylum Halobacteriota. It includes two genera, the alkaliphilic Methanonatronarchaeum and the neutrophilic Ca. Methanohalarchaeum. The former is represented by multiple...journal article 2022
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Khizhniak, Tatjana V. (author), Koenen, Michel (author), Bale, Nicole J. (author), Damsté, Jaap S.Sinninghe (author), Kublanov, Ilya V. (author)Several pure cultures of alkaliphilic haloaloarchaea were enriched and isolated from hypersaline soda lakes in southwestern Siberia using amylopectin and fructans as substrates. Phylogenomic analysis placed the isolates into two distinct groups within the class Halobacteria. Four isolates forming group 1 were closely related to a recently...journal article 2022
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Khijniak, Tatiana V. (author), Kolganova, Tatiana V. (author), Kublanov, Ilya V. (author)Extremely halophilic archaea (haloarchaea) of the class Halobacteria is<br/>a dominant group of aerobic heterotrophic prokaryotic communities in<br/>salt-saturated habitats, such as salt lakes and solar salterns. Most of the<br/>pure cultures of haloarchaea were enriched, isolated, and cultivated on<br/>rich soluble substrates such as amino...journal article 2022
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Muntyan, Maria S. (author), Viryasov, Mikhail B. (author), Sorokin, Dimitry Y. (author), Skulachev, Vladimir P. (author)As inhabitants of soda lakes, Thioalkalivibrio versutus are halo- and alkaliphilic bacteria that have previously been shown to respire with the first demonstrated Na+-translocating cytochrome-c oxidase (CO). The enzyme generates a sodium-motive force (Ds) as high as -270 mV across the bacterial plasma membrane. However, in these bacteria,...journal article 2022
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Sorokin, Dimitry Y. (author), Yakimov, Mikhail. M (author), Messina, Enzo (author), Merkel, Alexander. Y. (author), Koenen, Michel (author), Bale, Nicole J. (author), Sinnighe-Damste, Jaap S. (author)A pure culture of alkaliphilic haloarchaeon strain AArc-ST capable of anaerobic growth by carbohydrate-dependent sulfur respiration was obtained from hypersaline lakes in southwestern Siberia. According to phylogenetic analysis, AArc-ST formed a new genus level branch most related to the genus Natronoarchaeum in the order Halobacteriales. The...journal article 2022
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Khijniak, Tatiana V. (author), Zaharycheva, Alicia P. (author), Boueva, Olga V. (author), Ariskina, Elena V. (author), Bunk, Boyke (author), Spröer, Cathrin (author), Evtushenko, Lyudmila I. (author)During a cultural diversity survey on hydrolytic bacteria in saline alkaline soils, a hydrolytic actinobacterium strain ACPA39<sup>T</sup> was enriched and isolated in pure culture from a soda solonchak soil in southwestern Siberia. It forms a substrate mycelium with rod-shaped sporangia containing 1–3 exospores. The isolate is obligately...journal article 2022