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Salinity-induced succession of phototrophic communities in a southwestern Siberian soda lake during the solar activity cycle

Samylina, Olga S. (author), Kosyakova, Anastasia I. (author), Krylov, Artem A. (author), Sorokin, Dimitry Y. (author), Pimenov, Nikolay V. (author)

A variety of lakes located in the dry steppe area of southwestern Siberia are exposed to rapid climatic changes, including intra-century cycles with alternating dry and wet phases driven by solar activity. As a result, the salt lakes of that region experience significant fluctuations in water level and salinity, which have an essential impact...

journal article 2024

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Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales

Zhilina, Tatjana N. (author), Sorokin, Dimitry Y. (author), Toschakov, Stepan V. (author), Kublanov, Ilya V. (author), Zavarzina, Daria G. (author)

Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702 ^T is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum...

journal article 2023

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Selective enrichment on a wide polysaccharide spectrum allowed isolation of novel metabolic and taxonomic groups of haloarchaea from hypersaline lakes

Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Khijniak, Tatiana V. (author), Kolganova, Tatiana V. (author), Kublanov, Ilya V. (author)

Extremely halophilic archaea (haloarchaea) of the class Halobacteria is<br/>a dominant group of aerobic heterotrophic prokaryotic communities in<br/>salt-saturated habitats, such as salt lakes and solar salterns. Most of the<br/>pure cultures of haloarchaea were enriched, isolated, and cultivated on<br/>rich soluble substrates such as amino...

journal article 2022

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Natrarchaeobius chitinivorans gen. nov., sp. nov., and Natrarchaeobius halalkaliphilus sp. nov., alkaliphilic, chitin-utilizing haloarchaea from hypersaline alkaline lakes

Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Toshchakov, Stepan V. (author), Bale, Nicole J. (author), Sinninghe Damsté, Jaap S. (author), Khijniak, Tatiana V. (author), Kublanov, Ilya V. (author)

Two groups of alkaliphilic haloarchaea from hypersaline alkaline lakes in Central Asia, Egypt and North America were enriched and isolated in pure culture using chitin as growth substrate. These cultures, termed AArcht, were divided into two groups: group 1 which includes eleven isolates from highly alkaline soda lakes and group 2 which...

journal article 2019

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Diversity and distribution of sulfur oxidation-related genes in thioalkalivibrio, a genus of chemolithoautotrophic and haloalkaliphilic sulfur-oxidizing bacteria

Berben, Tom (author), Overmars, Lex (author), Sorokin, Dimitry Y. (author), Muyzer, Gerard (author)

Soda lakes are saline alkaline lakes characterized by high concentrations of sodium carbonate/bicarbonate which lead to a stable elevated pH (&gt;9), and moderate to extremely high salinity. Despite this combination of extreme conditions, biodiversity in soda lakes is high, and the presence of diverse microbial communities provides a driving...

journal article 2019

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Methanonatronarchaeum thermophilum gen. Nov., sp. nov. and ’Candidatus methanohalarchaeum thermophilum’, extremely halo(natrono)philic methyl-reducing methanogens from hypersaline lakes comprising a new euryarchaeal class Methanonatronarchaeia classis nov.

Sorokin, Dimitry Y. (author), Merkel, Alexander Y. (author), Abbas, B.A. (author), Makarova, Kira S. (author), Rijpstra, W. Irene C. (author), Koenen, M. (author), Sinninghe Damsté, Jaap S. (author), Galinski, Erwin A. (author), Koonin, Eugene V. (author), van Loosdrecht, Mark C.M. (author)

Methanogenic enrichments from hypersaline lakes at moderate thermophilic conditions have resulted in the cultivation of an unknown deep lineage of euryarchaeota related to the class Halobacteria. Eleven soda lake isolates and three salt lake enrichment cultures were methyl-reducing methanogens that utilize C₁ methylated compounds...

journal article 2018

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Effect of salinity on diazotrophic activity and microbial composition of phototrophic communities from Bitter-1 soda lake (Kulunda Steppe, Russia)

Namsaraev, Zorigto (author), Samylina, Olga (author), Sukhacheva, Marina (author), Borisenko, Gennadii (author), Sorokin, Dimitry Y. (author), Tourova, Tatiana (author)

Bitter-1 is a shallow hypersaline soda lake in Kulunda Steppe (Altai region, Russia). During a study period between 2005 and 2016, the salinity in the littoral area of the lake fluctuated within the range from 85 to 400 g/L (in July of each year). Light-dependent nitrogen fixation occurred in this lake up to the salt-saturating conditions....

journal article 2018

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Natronobiforma cellulositropha gen. nov., sp. nov., a novel haloalkaliphilic member of the family Natrialbaceae (class Halobacteria) from hypersaline alkaline lakes

Sorokin, Dimitry Y. (author), Khijniak, Tatiana V. (author), Kostrikina, Nadezhda A. (author), Elcheninov, Alexander G. (author), Toshchakov, Stepan V. (author), Bale, Nicole J. (author), Damsté, Jaap S.Sinninghe (author), Kublanov, Ilya V. (author)

Six strains of extremely halophilic and alkaliphilic euryarchaea were enriched and isolated in pure culture from surface brines and sediments of hypersaline alkaline lakes in various geographical locations with various forms of insoluble cellulose as growth substrate. The cells are mostly flat motile rods with a thin monolayer cell wall while...

journal article 2018

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Phenotypic and genomic properties of a novel deep-lineage haloalkaliphilic member of the phylum Balneolaeota from soda lakes possessing Na⁺-translocating proteorhodopsin

Sorokin, Dimitry Y. (author), Muntyan, Maria S. (author), Toshchakov, Stepan V. (author), Korzhenkov, Aleksei (author), Kublanov, Ilya V. (author)

Stable development of a heterotrophic bacterial satellite with a peculiar cell morphology has been observed in several enrichment cultures of haloalkaliphilic benthic filamentous cyanobacteria from a hypersaline soda lake in Kulunda Steppe (Altai, Russia). The organism was isolated in pure culture (strain Omega) using sonicated cyanobacterial...

journal article 2018

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Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments

Timmers, Peer H.A. (author), Vavourakis, Charlotte D. (author), Kleerebezem, R. (author), Sinninghe Damsté, Jaap S. (author), Muyzer, Gerard (author), Stams, Alfons J.M. (author), Sorokin, Dimitry Y. (author), Plugge, Caroline M. (author)

Anaerobic syntrophic acetate oxidation (SAO) is a thermodynamically unfavorable process involving a syntrophic acetate oxidizing bacterium (SAOB) that forms interspecies electron carriers (IECs). These IECs are consumed by syntrophic partners, typically hydrogenotrophic methanogenic archaea or sulfate reducing bacteria. In this work, the...

journal article 2018

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Analysis of the genes involved in thiocyanate oxidation during growth in continuous culture of the haloalkaliphilic sulfur-oxidizing bacterium Thioalkalivibrio thiocyanoxidans ARh 2T using transcriptomics

Berben, Tom (author), Balkema, Cherel (author), Sorokin, Dimitry Y. (author), Muyzer, Gerard (author)

Thiocyanate (N=C-S-) is a moderately toxic, inorganic sulfur compound. It occurs naturally as a by-product of the degradation of glucosinolatecontaining plants and is produced industrially in a number of mining processes. Currently, two pathways for the primary degradation of thiocyanate in bacteria are recognized, the carbonyl sulfide...

journal article 2017

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Comparative genome analysis of three thiocyanate oxidizing Thioalkalivibrio species isolated from soda lakes

Berben, Tom (author), Overmars, Lex (author), Sorokin, Dimitry Y. (author), Muyzer, Gerard (author)

Thiocyanate is a C1 compound containing carbon, nitrogen, and sulfur. It is a (by)product in a number of natural and industrial processes. Because thiocyanate is toxic to many organisms, including humans, its removal from industrial waste streams is an important problem. Although a number of bacteria can use thiocyanate as a nitrogen source,...

journal article 2017

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Desulfonatronospira sulfatiphila sp. Nov., and Desulfitispora elongata sp. nov., two novel haloalkaliphilic sulfidogenic bacteria from soda lakes

Sorokin, Dimitry Y. (author), Chernyh, Nikolai A. (author)

Two novel haloalkaliphilic bacteria with dissimilatory sulfidogenic metabolism were recovered from syntrophic associations obtained from anaerobic sediments of hypersaline soda lakes in Kulunda Steppe (Altai, Russia). Strain ASO3-2^T was a member of a sulfidogenic syntrophic association oxidizing acetate at extremely haloalkaline...

journal article 2017

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Natronospira proteinivora gen. nov., sp. nov, an extremely salttolerant, alkaliphilic gammaproteobacterium from hypersaline soda lakes

Sorokin, Dimitry Y. (author), Kublanov, Ilya V. (author), Khijniak, Tatiana V. (author)

Brine samples from Kulunda Steppe soda lakes (Altai, Russia) were inoculated into a hypersaline alkaline mineral medium with β-keratin (chicken feather) as a substrate. The micro-organisms dominating the enrichment culture were isolated by limiting serial dilution on the same medium with casein as a substrate. The cells of strain BSker1<sup>T...

journal article 2017

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Sulfur and Oxygen Isotope Fractionation During Bacterial Sulfur Disproportionation Under Anaerobic Haloalkaline Conditions

Poser, Alexander (author), Vogt, Carsten (author), Knöller, Kay (author), Sorokin, Dimitry Y. (author), Finster, Kai W. (author), Richnow, Hans H. (author)

Sulfur and oxygen isotope fractionation of elemental sulfur disproportionation at anaerobic haloalkaline conditions was evaluated for the first time. Isotope enrichment factors of the strains Desulfurivibrio alkaliphilus and Dethiobacter alkaliphilus growing at pH 9 or 10 were −0.9‰ to −1‰ for sulfide (³⁴ϵ), +3.6‰ to +4.7‰ for...

journal article 2016

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‘Candidatus Desulfonatronobulbus propionicus’: a first haloalkaliphilic member of the order Syntrophobacterales from soda lakes

Sorokin, Dimitry Y. (author), Chernyh, N. A. (author)

Propionate can be directly oxidized anaerobically with sulfate as e-acceptor at haloalkaline conditions either incompletely to acetate (an example is Desulfobulbus alkaliphilus), or completely (for example by the members of genus Desulfonatronobacter). An enrichment with propionate at methanogenic conditions (without sulfate) inoculated with...

journal article 2016

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Desulfurispira natronophila gen. nov. sp. nov.: An obligately anaerobic dissimilatory sulfur-reducing bacterium from soda lakes

Sorokin, D.Y. (author), Muyzer, G. (author)

Anaerobic enrichment cultures with elemental sulfur as electron acceptor and either acetate or propionate as electron donor and carbon source at pH 10 and moderate salinity inoculated with sediments from soda lakes in Kulunda Steppe (Altai, Russia) resulted in the isolation of two novel members of the bacterial phylum Chrysiogenetes. The...

journal article 2010

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Haloalkaliphilic spore-forming sulfidogens from soda lake sediments and description of Desulfitispora alkaliphila gen. nov., sp. nov.

Sorokin, D.Y. (author), Muyzer, G. (author)

An anaerobic enrichment with pyruvate as electron donor and thiosulfate at pH 10 and 0.6 M Na? inoculated with pasteurized soda lake sediments resulted in a sulfidogenic coculture of two morphotypes of obligately anaerobic haloalkaliphilic endospore-forming clostridia, which were further isolated in pure culture. Strain AHT16 was a thin long rod...

journal article 2010

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Propionate and butyrate dependent bacterial sulfate reduction at extremely haloalkaline conditions and description of Desulfobotulus alkaliphilus sp. nov.

Sorokin, D.Y. (author), Detkova, E.N. (author), Muyzer, G. (author)

Evidence on the utilization of simple fatty acids by sulfate-reducing bacteria (SRB) at extremely haloalkaline conditions are practically absent, except for a single case of syntrophy by Desulfonatronum on acetate. Our experiments with sediments from soda lakes of Kulunda Steppe (Altai, Russia) showed sulfide production with sulfate as electron...

journal article 2009

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Bacterial dissimilatory MnO2 reduction at extremely haloalkaline conditions

Sorokin, D.Y. (author), Muyzer, G. (author)

A possibility of dissimilatory MnO2 reduction at extremely high salt and pH was studied in sediments from hypersaline alkaline lakes in Kulunda Steppe (Altai, Russia). Experiments with anaerobic sediment slurries demonstrated a relatively rapid reduction of colloidal MnO2 in the presence of acetate and formate as electron donor at in situ...

journal article 2009