DNA sensing with nanopores in graphene nanoribbons

The information that can be extracted from DNA base sequences, is important for our understanding of biology, for insights in disease, and the way we practice medicine. The technology that one uses to determine DNA-base sequences is called ‘DNA sequencing’. A portable device that enables fast and accurate sequencing, can potentially greatly...

Probing DNA Translocations with Inplane Current Signals in a Graphene Nanoribbon with a Nanopore

Many theoretical studies predict that DNA sequencing should be feasible by monitoring the transverse current through a graphene nanoribbon while a DNA molecule translocates through a nanopore in that ribbon. Such a readout would benefit from the special transport properties of graphene, provide ultimate spatial resolution because of the single...

Detection of CRISPR-dCas9 on DNA with Solid-State Nanopores

Solid-state nanopores have emerged as promising platforms for biosensing including diagnostics for disease detection. Here we show nanopore experiments that detect CRISPR-dCas9, a sequence-specific RNA-guided protein system that specifically binds to a target DNA sequence. While CRISPR-Cas9 is acclaimed for its gene editing potential, the...

Through-membrane electron-beam lithography for ultrathin membrane applications

We present a technique to fabricate ultrathin (down to 20 nm) uniform electron transparent windows at dedicated locations in a SiN membrane for in situ transmission electron microscopy experiments. An electron-beam (e-beam) resist is spray-coated on the backside of the membrane in a KOH-etched cavity in silicon which is patterned using...

Graphene nanodevices for DNA sequencing

Fast, cheap, and reliable DNA sequencing could be one of the most disruptive innovations of this decade, as it will pave the way for personalized medicine. In pursuit of such technology, a variety of nanotechnology-based approaches have been explored and established, including sequencing with nanopores. Owing to its unique structure and...

Mapping Geotechnical Risk for Infrastructural Works in Deltaic Areas

This paper presents method and first results of a study to quantify and communicate geotechnical risk for highway construction on soft soil and large building pits associated with infrastructural works in the Netherlands. A set of easy-to-read maps will inform the end users, geotechnical consultants at the Dutch Ministry of Infrastructure and...

Geotechnical Engineering in Contracts for Civil Projects

Geo-Impuls is a Dutch nationwide programme with the aim to reduce geotechnical related failures in projects. During the last 5 years, a large number of teams have been working within three themes (techniques, communications and contracts) on a procedure for coping with the natural uncertainties of the Dutch subsoil. The large variation in the...

Geo Risk Management Results for a Public Client Organization

Rijkswaterstaat is the executive agency of the Ministry of Infrastructure and the Environment, responsible for development and maintenance of the Dutch main road network, main waterway network and main water systems. Managing and realizing these networks, not only within time, budget, safety margins and quality standards, but also with a minimum...

In-situ product removal by membrane extraction

In bioproduction processes of chemicals and pharmaceuticals, downstream processing usually is a significant cost factor. The products require a high purity (especially biopharmaceutical products), therefore, the process usually contains a large number of separation steps. Moreover, the high costs in downstream processing are caused by the fact...

Preparing a long term management plan for the future of the Slufter: An analysis of the functional, spatial and geotechnical possibilities

The Slufter is a large scale disposal facility for contaminated dredged material from the Dutch rivers, channels and harbour basins. It is located in the Rotterdam harbour, on the south-western tip of the Maasvlakte. Since the Slufter was built in 1987, the supply of contaminated dredged material has decreased significantly. As a result, it will...

Jetty design at Rio Araranguá

Master project report. The hydraulic efficiency of the river Araranguá will be increased by the construction of jetties at the mouth of the river. The river tends to migrate from south to north which decreases the hydraulic efficiency and causes flooding upstream during events of high discharge. To stop the migration the construction of jetties...

Investigation of the response of a turret type calm buoy in sub-arctic conditions

Development of the Yoke Quay Mooring Concept (YQM) – Weathervaning LNG Terminal in Shallow Water

Grondstoffenverwerking: Afbraak en opbouw

The cad environment of the national aerospace laboratory NLR, the Netherlands

CAD systems can be used as carrier of knowledge from applied technical research. As such they can facilitate knowledge transfer from institutes for scientific technical research to industry. It is essential that CAD systems form part of a consistent infrastructure for information processing in the concerning industry. As a result it is required...

EPIDAS: A multi-discipline and integrated facility for management and evaluation of engineering data

The paper describes EDIPAS, the multi-discipline and integrated facility for management, and analysis and presentation of engineering data. EDIPAS has been designed to serve: - as a framework to transfer data between computer programs used in the various design disciplines; - as a standard set of functions for analysis and presentation of data,...

EDIPAS : a general approach to engineering data management and analysis to wind-tunnel testing

In the design of vehicles, the application of experimental and computational tools, including geometry handling, are increasingly important. In this situation organisations need facilities that: -serve as a neutral transfer point between the various tools and between the various disciplines involved in the design process; -provide a standard set...

Funktionele analyse van de automatiseringsbehoeftenen technisch concept voor de verdere ontwikkeling van de computer-infrastructuur bij het NLR

Om haar taken doeltreffend te kunnen uitvoeren was in de zeventiger jaren voor het NLR een ver doorgevoerde centralisering nodig van funkties op de centrale computer in de Noordoostpolder. Deze centralisering werd mogelijk gemaakt door de opzet van een computernetwerk rondom deze centrale computer. Voor de jaren tachtig gelden de eisen, welke...