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Naapuri, Janne M. (author), Wagner, Philip K. (author), Hollmann, F. (author), Deska, Jan (author)Vanadate-dependent chloroperoxidase from Curvularia inaequalis catalyzes 5-endo-trig bromocyclizations of α-allenols to produce valuable halofunctionalized furans as versatile synthetic building blocks. In contrast to other haloperoxidases, also the more challenging 5-exo-trig halocyclizations of γ-allenols succeed with this system even...journal article 2022
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Xu, X. (author), Brasselet, H.L.Y. (author), Jongkind, E.P.J. (author), Alcalde, Miguel (author), Paul, C.E. (author), Hollmann, F. (author)In this study, we developed a new bienzymatic reaction to produce enantioenriched phenylethanols. In a first step, the recombinant, unspecific peroxygenase from Agrocybe aegerita (rAaeUPO) was used to oxidise ethylbenzene and its derivatives to the corresponding ketones (prochiral intermediates) followed by enantioselective reduction into the...journal article 2022
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Beltrán-Nogal, Alejandro (author), Sánchez-Moreno, Israel (author), Méndez-Sánchez, Daniel (author), Gómez de Santos, Patricia (author), Hollmann, F. (author), Alcalde, Miguel (author)The selective insertion of oxygen into non-activated organic molecules has to date been considered of utmost importance to synthesize existing and next generation industrial chemicals or pharmaceuticals. In this respect, the minimal requirements and high activity of fungal unspecific peroxygenases (UPOs) situate them as the jewel in the crown...review 2022
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Zippilli, C. (author), Bartolome, Miguel Jimenez (author), Hilberath, T. (author), Botta, Lorenzo (author), Hollmann, F. (author), Saladino, Raffaele (author)A photochemoenzymatic halodecarboxylation of ferulic acid was achieved using vanadate-dependent chloroperoxidase as (bio)catalyst and oxygen and organic solvent as sole stoichiometric reagents in a biphasic system. Performance and selectivity were improved through a phase transfer catalyst, reaching a turnover number of 660.000 for the enzyme...journal article 2022
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Hwang, Se Yeun (author), Song, Dayoon (author), Seo, Eun Ji (author), Hollmann, F. (author), You, Youngmin (author), Park, Jin Byung (author)Photobiocatalysis is a growing field of biocatalysis. Especially light-driven enzyme catalysis has contributed significantly to expanding the scope of synthetic organic chemistry. However, photoenzymes usually utilise a rather narrow wavelength range of visible (sun)light. Triplet–triplet annihilation-based upconversion (TTA-UC) of long...journal article 2022
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Ma, Yunjian (author), Liang, Hongjing (author), Zhao, Zexin (author), Wu, Bin (author), Lan, Dongming (author), Hollmann, F. (author), Wang, Yonghua (author)Unspecific peroxygenases (UPOs, EC 1.11.2.1) are promising oxyfunctionalization catalysts because of their unique stereoselectivity. However, so far only a few UPOs have been reported. In this study, gene mining was used to identify a gene from Galerina marginata that coded for a novel UPO (GmaUPO). GmaUPO was expressed in Pichia pastoris X...journal article 2022
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Yun, Chul Ho (author), Kim, Jinhyun (author), Hollmann, F. (author), Park, Chan Beum (author)Enzymes are the catalyst of choice for highly selective reactions, offering nature-inspired approaches for sustainable chemical synthesis. Oxidative enzymes (e.g., monooxygenases, peroxygenases, oxidases, or dehydrogenases) catalyze a variety of enantioselective oxyfunctionalization and dehydrogenation reactions under mild conditions. To...review 2022
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Benincá, Luiza A.D. (author), França, Alexandre S. (author), Brêda, Gabriela C. (author), Leão, Raquel A.C. (author), Almeida, Rodrigo V. (author), Hollmann, F. (author), de Souza, Rodrigo O.M.A. (author)The fatty acid photodecarboxylase from Chlorella variabilis NC64A (CvFAP) promotes the elimination of CO<sub>2</sub> from fatty acids (C<sub>n</sub>) producing the corresponding hydrocarbon (<sub>Cn-1</sub>). Therefore, this enzyme is of great biotechnological interest since it can be used in alternative biofuel production routes matching the...journal article 2022
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Zhong, Xuanru (author), Ma, Yunjian (author), Zhang, Xizhen (author), Zhang, Jiahui (author), Wu, Bin (author), Hollmann, F. (author), Wang, Yonghua (author)Hydrocarbon synthesis from (waste)oils enabled by a cascade of lipase-catalysed hydrolysis and decarboxylase-catalysed decarboxylation has become an active area of research en route to alternative, biobased fuels. However, Poor substrate transport efficiency is a major issue causing low reaction rates. This study focused on a protein self...journal article 2022
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Yoon, Jaeho (author), Jang, Hanhwi (author), Oh, Min Wook (author), Hilberath, T. (author), Hollmann, F. (author), Jung, Yeon Sik (author), Park, Chan Beum (author)Heat is a fundamental feedstock, where more than 80% of global energy comes from fossil-based heating process. However, it is mostly wasted due to a lack of proper techniques of utilizing the low-quality waste heat (<100 °C). Here we report thermoelectrobiocatalytic chemical conversion systems for heat-fueled, enzyme-catalyzed...journal article 2022
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Sang, Xianke (author), Tong, Feifei (author), Zeng, Zhigang (author), Wu, Minghu (author), Yuan, Bo (author), Sun, Zhoutong (author), Sheng, Xiang (author), Hollmann, F. (author), Zhang, W. (author)Propargylic alcohols and amines are versatile building blocks in organic synthesis. We demonstrate a straightforward enzymatic cascade to synthesize enantiomerically pure propargylic alcohols and amines from readily available racemic starting materials. In the first step, the peroxygenase from Agrocybe aegerita converted the racemic...journal article 2022
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Li, Zongquan (author), Ma, Yunjian (author), Hollmann, F. (author), Wang, Yonghua (author)Carvol and carvone are oxidation products from the natural product limonene. They are important raw materials for the flavours and fragrances industry and also act as pharmaceutical active ingredients. Orange waste peels possibly represent an attractive source for limonene, but studies on valorizing orange peel wastes are rare. In this study,...journal article 2022
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Li, Huanhuan (author), Younes, S.H.H. (author), Chen, Shaohang (author), Duan, Peigao (author), Cui, Chengsen (author), Wever, Ron (author), Zhang, Wuyuan (author), Hollmann, F. (author)In this contribution, we report chemoenzymatic bromodecarboxylation (Hunsdiecker-type) of α,ß-unsaturated carboxylic acids. The extraordinarily robust chloroperoxidase from Curvularia inaequalis (CiVCPO) generated hypobromite from H2O2 and bromide, which then spontaneously reacted with a broad range of unsaturated carboxylic acids and yielded...journal article 2022
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Kim, Jinhyun (author), Um, Yunna (author), Han, Seunghyun (author), Hilberath, T. (author), Kim, Yong Hwan (author), Hollmann, F. (author), Park, Chan Beum (author)The pulp and paper manufacturers generate approximately 50 million metric tons of lignin per annum, most of which has been abandoned or incinerated because of lignin's recalcitrant nature. Here, we report bias-free photoelectrochemical (PEC) oxidation of lignin coupled with asymmetric hydrogenation of C=C bonds. The PEC platform consists of a...journal article 2022
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Yang, Liu (author), Ma, Yunjian (author), Chen, Yebao (author), Hollmann, F. (author), Wang, Yonghua (author)Phthalic acid esters (PAEs) are widely used as plastic additives to increase the flexibility and durability of plastics. Constantly leaching out from plastics, PAEs are ubiquitously found in the environment. As PAEs exhibit biological activities such as being endocrine disruptive, the quest for efficient degradation strategies continues. Here...journal article 2022
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Chanquia, Santiago Nahuel (author), Benfeldt, Frederik Vig (author), Petrovai, Noémi (author), Santner, Paul (author), Hollmann, F. (author), Eser, Bekir Engin (author), Kara, Selin (author)Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the...journal article 2022
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- Han, Jeong Woo (author), Hollmann, F. (author), Luque, Rafael (author), Song, In Kyu (author), Talarico, Giovanni (author), Tatsumi, Takashi (author), Yan, Ning (author) journal article 2022
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Hollmann, F. (author), Ulber, Roland (author), Holtmann, Dirk (author)In chemical process engineering, process intensification (PI) has proven itself as a method that resulted very often in processes with an at least doubled process performance. In recent years, the PI techniques have found more and more applications in biotechnology. Exemplary continuous processes, single-use reactors, electrobiotechnology and...review 2021
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Holtmann, Dirk (author), Hollmann, F. (author)Process intensification aims at enabling bridging the gap between fundamental research such as identification of new catalysts and reactions and their implementation in industrial environments. Especially the field of biocatalysis has seen some tremendous improvements and the development of new tools and approaches to bridge this gap. In this...journal article 2021
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Alagumalai, Avinash (author), Mahian, Omid (author), Hollmann, F. (author), Zhang, Wuyuan (author)Versatile bio-derived catalysts have been under dynamic investigation as potential substitutes to conventional chemical catalysts for sustainable biodiesel production. This is because of their unique, low-cost benefits and production processes that are environmentally and economically acceptable. This critical review aspires to present a...review 2021