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Mannina, Giorgio (author), Chandran, Kartik (author), Capodici, Marco (author), Cosenza, Alida (author), Di Trapani, Daniele (author), van Loosdrecht, Mark C.M. (author)This study aimed at evaluating the nitrous oxide (N2O) emissions from membrane bioreactors (MBRs) for wastewater treatment. The study investigated the N2O emissions considering multiple influential factors over a two-year period: (i) different MBR based process configurations; (ii) wastewater composition (municipal or industrial); (iii)...journal article 2018
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Sanawar, H. (author), Pinel, I.S.M. (author), Farhat, N. M. (author), Bucs, Sz S. (author), Zlopasa, J. (author), Kruithof, J. C. (author), Witkamp, G.J. (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Chemical cleaning is routinely performed in reverse osmosis (RO) plants for the regeneration of RO membranes that suffer from biofouling problems. The potential of urea as a chaotropic agent to enhance the solubilization of biofilm proteins has been reported briefly in the literature. In this paper the efficiency of urea cleaning for RO...journal article 2018
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Conthe Calvo, M. (author), Wittorf, Lea (author), Kuenen, J.G. (author), Kleerebezem, R. (author), Hallin, Sara (author), van Loosdrecht, Mark C.M. (author)Nitrous oxide (N<sub>2</sub>O) reducing microorganisms may be key in the mitigation of N<sub>2</sub>O emissions from managed ecosystems. However, there is still no clear understanding of the physiological and bioenergetic implications of microorganisms possessing either of the two N<sub>2</sub>O reductase genes (nosZ), clade I and the more...journal article 2018
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Lin, Y. (author), Reino, Clara (author), Carrera, Julián (author), Pérez, Julio (author), van Loosdrecht, Mark C.M. (author)A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different glycoconjugates. Its...journal article 2018
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Conthe Calvo, M. (author), Wittorf, Lea (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Hallin, Sara (author)Reduction of the greenhouse gas N<sub>2</sub>O to N<sub>2</sub> is a trait among denitrifying and non-denitrifying microorganisms having an N<sub>2</sub>O reductase, encoded by nosZ. The nosZ phylogeny has two major clades, I and II, and physiological differences among organisms within the clades may affect N<sub>2</sub>O emissions from...journal article 2018
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Wang, Zhongwei (author), Dunne, Aislinn (author), van Loosdrecht, Mark C.M. (author), Saikaly, Pascal E. (author)Saline wastewater is known to affect the performance of phosphate-accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) process. However, studies comparing the effect of salinity on different PAO clades are lacking. In this study, 'Candidatus Accumulibacter phosphatis' Clade I and II (hereafter referred to as PAOI and...journal article 2018
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Sorokin, Dimitry Y. (author), Merkel, Alexander Y. (author), Abbas, B.A. (author), Makarova, Kira S. (author), Rijpstra, W. Irene C. (author), Koenen, M. (author), Sinninghe Damsté, Jaap S. (author), Galinski, Erwin A. (author), Koonin, Eugene V. (author), van Loosdrecht, Mark C.M. (author)Methanogenic enrichments from hypersaline lakes at moderate thermophilic conditions have resulted in the cultivation of an unknown deep lineage of euryarchaeota related to the class Halobacteria. Eleven soda lake isolates and three salt lake enrichment cultures were methyl-reducing methanogens that utilize C<sub>1</sub> methylated compounds...journal article 2018
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Le, Quan H. (author), Verheijen, Peter J.T. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)A stepwise experimental design procedure to obtain reliable data from wastewater treatment plants (WWTPs) was developed. The proposed procedure aims at determining sets of additional measurements (besides available ones) that guarantee the identifiability of key process variables, which means that their value can be calculated from other,...journal article 2018
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Soler Jofra, A. (author), Wang, R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)A lab-scale partial nitritation granular sludge air-lift reactor was operated in continuous mode treating low strength synthetic medium (influent ca. 50 mg-N-NH<sub>4</sub> <sup>+</sup>/L). Granules were initially stratified with AOB in the external shell and NOB in the inner core at 20 °C. Once temperature was decreased progressively from 20...journal article 2019
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Conthe Calvo, M. (author), Lycus, Pawel (author), Arntzen, Magnus (author), Ramos da Silva, Aline (author), Frostegård, Åsa (author), Bakken, Lars R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)The strong greenhouse gas nitrous oxide (N<sub>2</sub>O) can be emitted from wastewater treatment systems as a byproduct of ammonium oxidation and as the last intermediate in the stepwise reduction of nitrate to N<sub>2</sub> by denitrifying organisms. A potential strategy to reduce N<sub>2</sub>O emissions would be to enhance the activity of...journal article 2019
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Suresh Kumar, P. (author), Korving, Leon (author), van Loosdrecht, Mark C.M. (author), Witkamp, G.J. (author)Eutrophication and the resulting formation of harmful algal blooms (HAB) causes huge economic and environmental damages. Phosphorus (P) from sewage effluent and agricultural run-off has been identified as a major cause for eutrophication. Phosphorous concentrations greater than 100 μg P/L are usually considered high enough to cause...review 2019
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Sanawar, Huma (author), Bucs, Szilárd S. (author), Pot, Martin A. (author), Zlopasa, J. (author), Farhat, Nadia M. (author), Witkamp, Geert Jan (author), Kruithof, Joop C. (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Routine chemical cleaning with the combined use of sodium hydroxide (NaOH) and hydrochloric acid (HCl) is carried out as a means of biofouling control in reverse osmosis (RO) membranes. The novelty of the research presented herein is in the application of urea, instead of NaOH, as a chemical cleaning agent to full-scale spiral-wound RO...journal article 2019
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Rombouts, J.L. (author), Mos, G. (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or...journal article 2019
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Rubio Rincon, F.J. (author), Welles, L. (author), Lopez Vazquez, Carlos (author), Abbas, B.A. (author), van Loosdrecht, Mark C.M. (author), Brdjanovic, Damir (author)Candidatus Accumulibacter phosphatis is in general presented as the dominant organism responsible for the biological removal of phosphorus in activated sludge wastewater treatment plants. Lab-scale enhanced biological phosphorus removal (EBPR) studies, usually use acetate as carbon source. However, the complexity of the carbon sources present in...journal article 2019
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Suresh Kumar, P. (author), Korving, Leon (author), Keesman, Karel J. (author), van Loosdrecht, Mark C.M. (author), Witkamp, G.J. (author)Phosphate is a vital nutrient but its presence in surface waters even at very low concentrations can lead to eutrophication. Adsorption is often suggested as a step for reducing phosphate down to very low concentrations. Porous metal oxides can be used as granular adsorbents that have a high surface area and hence a high adsorption capacity....journal article 2019
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- Sorokin, Dimitry Y. (author), Makarova, Kira S. (author), Abbas, B.A. (author), Ferrer, Manuel (author), Golyshin, Peter N. (author), Galinski, Erwin A. (author), Ciorda, Sergio (author), Mena, María Carmen (author), van Loosdrecht, Mark C.M. (author) journal article 2019
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Jafari, Morez (author), Derlon, Nicolas (author), Desmond, Peter (author), van Loosdrecht, Mark C.M. (author), Morgenroth, Eberhard (author), Picioreanu, C. (author)Poroelastic fluid-structure interaction models were coupled to experimental data to determine the effects of biofilm spatial distribution of mechanical and hydraulic properties on the biofilm hydraulic resistance and compressibility in membrane filtration processes. Biofilms were cultivated on ultrafiltration membranes for 20 and 30 days...journal article 2019
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Felz, S. (author), Vermeulen, Pascalle (author), van Loosdrecht, Mark C.M. (author), Lin, Yue Mei (author)Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on...journal article 2019
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de Graaff, D.R. (author), Felz, S. (author), Neu, Thomas R. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS). Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by...journal article 2019
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Olavarria, Karel (author), Fina, Albert (author), Velasco Alvarez, M.I. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Biotechnological industry strives to develop anaerobic bioprocesses fueled by abundant and cheap carbon sources, like sucrose. However, oxygen-limiting conditions often lead to by-product formation and reduced ATP yields. While by-product formation is typically decreased by gene deletion, the breakdown of oligosaccharides with inorganic...journal article 2019