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4D printed shape-shifting biomaterials for tissue engineering and regenerative medicine applications

Kalogeropoulou, Maria (author), Diaz Payno, P.J. (author), Mirzaali, Mohammad J. (author), van Osch, G.J.V.M. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

The existing 3D printing methods exhibit certain fabrication-dependent limitations for printing curved constructs that are relevant for many tissues. Four-dimensional (4D) printing is an emerging technology that is expected to revolutionize the field of tissue engineering and regenerative medicine (TERM). 4D printing is based on 3D printing,...

review 2024

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Incorporating strontium enriched amorphous calcium phosphate granules in collagen/collagen-magnesium-hydroxyapatite osteochondral scaffolds improves subchondral bone repair

Xu, J. (author), Vecstaudza, Jana (author), Wesdorp, Marinus A. (author), Labberté, Margot (author), Salerno, Manuela (author), Kok, Joeri (author), van Rietbergen, Bert (author), van Osch, G.J.V.M. (author), Locs, Janis (author), Brama, Pieter A.J. (author)

Osteochondral defect repair with a collagen/collagen-magnesium-hydroxyapatite (Col/Col-Mg-HAp) scaffold has demonstrated good clinical results. However, subchondral bone repair remained suboptimal, potentially leading to damage to the regenerated overlying neocartilage. This study aimed to improve the bone repair potential of this scaffold by...

journal article 2024

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Glycosaminoglycan-Mediated Interactions in Articular, Auricular, Meniscal, and Nasal Cartilage

Rathnayake, Manula S.B. (author), Boos, Manuela A. (author), Farrugia, Brooke L. (author), van Osch, G.J.V.M. (author), Stok, Kathryn S. (author)

Glycosaminoglycans (GAGs) are ubiquitous components in the cartilage extracellular matrix (ECM). Ultrastructural arrangement of ECM and GAG-mediated interactions with collagen are known to govern the mechanics in articular cartilage, but these interactions are less clear in other cartilage types. Therefore, this article reviews the current...

review 2024

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Biomimetic Approaches for the Design and Fabrication of Bone-to-Soft Tissue Interfaces

Pitta Kruize, Carlos (author), Panahkhahi, S. (author), Putra, N.E. (author), Diaz Payno, P.J. (author), van Osch, G.J.V.M. (author), Zadpoor, A.A. (author), Mirzaali, Mohammad J. (author)

Bone-to-soft tissue interfaces are responsible for transferring loads between tissues with significantly dissimilar material properties. The examples of connective soft tissues are ligaments, tendons, and cartilages. Such natural tissue interfaces have unique microstructural properties and characteristics which avoid the abrupt transitions...

review 2023

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Effectiveness of BMP-2 and PDGF-BB Adsorption onto a Collagen/Collagen-Magnesium-Hydroxyapatite Scaffold in Weight-Bearing and Non-Weight-Bearing Osteochondral Defect Bone Repair: In Vitro, Ex Vivo and In Vivo Evaluation

Xu, J. (author), Fahmy-Garcia, Shorouk (author), Wesdorp, Marinus A. (author), Forte, Lucia (author), De Luca, Claudio (author), Filardo, Giuseppe (author), Labberté, Margot (author), Kok, Joeri (author), Nickel, Joachim (author), van Osch, G.J.V.M. (author)

Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet...

journal article 2023

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Engineered biochemical cues of regenerative biomaterials to enhance endogenous stem/progenitor cells (ESPCs)-mediated articular cartilage repair

Zhou, Liangbin (author), Xu, J. (author), Schwab, Andrea (author), Tong, Wenxue (author), Zheng, Lizhen (author), Li, Zhuo (author), Xu, Shunxiang (author), van Osch, G.J.V.M. (author), Wen, Chunyi (author), Qin, Ling (author)

As a highly specialized shock-absorbing connective tissue, articular cartilage (AC) has very limited self-repair capacity after traumatic injuries, posing a heavy socioeconomic burden. Common clinical therapies for small- to medium-size focal AC defects are well-developed endogenous repair and cell-based strategies, including microfracture,...

review 2023

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MR Vascular Fingerprinting with Hybrid Gradient–Spin Echo Dynamic Susceptibility Contrast MRI for Characterization of Microvasculature in Gliomas

Venugopal, Krishnapriya (author), Arzanforoosh, Fatemeh (author), van Dorth, Daniëlle (author), Smits, M. (author), van Osch, Matthias J.P. (author), Hernandez-Tamames, J.A. (author), Warnert, E.A.H. (author), Poot, Dirk H.J. (author)

Characterization of tumor microvasculature is important in tumor assessment and studying treatment response. This is possible by acquiring vascular biomarkers with magnetic resonance imaging (MRI) based on dynamic susceptibility contrast (DSC). We propose magnetic resonance vascular fingerprinting (MRVF) for hybrid echo planar imaging (HEPI)...

journal article 2023

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In Vitro Mineralisation of Tissue-Engineered Cartilage Reduces Endothelial Cell Migration, Proliferation and Tube Formation

Ji, Encheng (author), Leijsten, Lieke (author), Witte-Bouma, Janneke (author), Rouchon, Adelin (author), Di Maggio, Nunzia (author), Banfi, Andrea (author), van Osch, G.J.V.M. (author), Farrell, Eric (author), Lolli, Andrea (author)

Tissue engineering bone via endochondral ossification requires the generation of a cartilage template which undergoes vascularisation and remodelling. While this is a promising route for bone repair, achieving effective cartilage vascularisation remains a challenge. Here, we investigated how mineralisation of tissue-engineered cartilage...

journal article 2023

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Women’s contribution to stem cell research for osteoarthritis: an opinion paper

Velot, Émilie (author), Balmayor, Elizabeth R. (author), Bertoni, Lélia (author), Chubinskaya, Susan (author), Cicuttini, Flavia (author), de Girolamo, Laura (author), Demoor, Magali (author), van Osch, G.J.V.M. (author), Cucchiarini, Magali (author)

review 2023

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Rational positioning of 3D-printed voxels to realize high-fidelity multifunctional soft-hard interfaces

Cruz Saldivar, M. (author), Veeger, Robin Petrus Elias (author), Tay, E.T.W.S. (author), Fenu, M. (author), Klimopoulou, M. (author), van Osch, G.J.V.M. (author), Fratila-Apachitei, E.L. (author), Doubrovski, E.L. (author), Mirzaali, Mohammad J. (author), Zadpoor, A.A. (author)

Living organisms use functional gradients (FGs) to interface hard and soft materials (e.g., bone and tendon), a strategy with engineering potential. Past attempts involving hard (or soft) phase ratio variation have led to mechanical property inaccuracies because of microscale-material macroscale-property nonlinearity. This study examines 3D...

journal article 2023

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Addition of heparin binding sites strongly increases the bone forming capabilities of BMP9 in vivo

Siverino, Claudia (author), Fahmy-Garcia, Shorouk (author), Niklaus, Viktoria (author), Kops, Nicole (author), Dolcini, Laura (author), Misciagna, Massimiliano Maraglino (author), Ridwan, Yanto (author), Farrell, Eric (author), van Osch, G.J.V.M. (author), Nickel, Joachim (author)

Bone Morphogenetic proteins (BMPs) like BMP2 and BMP7 have shown great potential in the treatment of severe bone defects. In recent in vitro studies, BMP9 revealed the highest osteogenic potential compared to other BMPs, possibly due to its unique signaling pathways that differs from other osteogenic BMPs. However, in vivo the bone forming...

journal article 2023

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Senescence during early differentiation reduced the chondrogenic differentiation capacity of mesenchymal progenitor cells

Voskamp, Chantal (author), Koevoet, Wendy J.L.M. (author), van Osch, G.J.V.M. (author), Narcisi, Roberto (author)

Introduction: Mesenchymal stromal/progenitor cells (MSCs) are promising for cartilage cell-based therapies due to their chondrogenic differentiation capacity. However, MSCs can become senescent during in vitro expansion, a state characterized by stable cell cycle arrest, metabolic alterations, and substantial changes in the gene expression and...

journal article 2023

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Improved reliability of perfusion estimation in dynamic susceptibility contrast MRI by using the arterial input function from dynamic contrast enhanced MRI

Tseng, C. (author), Jaspers, Jaap (author), Romero, Alejandra Mendez (author), Wielopolski, Piotr (author), Smits, M. (author), van Osch, Matthias J.P. (author), Vos, F.M. (author)

The arterial input function (AIF) plays a crucial role in estimating quantitative perfusion properties from dynamic susceptibility contrast (DSC) MRI. An important issue, however, is that measuring the AIF in absolute contrast-agent concentrations is challenging, due to uncertainty in relation to the measured (Formula presented.) -weighted...

journal article 2023

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Modulating design parameters to drive cell invasion into hydrogels for osteochondral tissue formation

Schwab, Andrea (author), Wesdorp, Marinus A. (author), Xu, J. (author), Abinzano, Florencia (author), Loebel, Claudia (author), Levato, Riccardo (author), Eglin, David (author), Narcisi, Roberto (author), Malda, Jos (author), van Osch, G.J.V.M. (author)

Background: The use of acellular hydrogels to repair osteochondral defects requires cells to first invade the biomaterial and then to deposit extracellular matrix for tissue regeneration. Due to the diverse physicochemical properties of engineered hydrogels, the specific properties that allow or even improve the behaviour of cells are not yet...

journal article 2023

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Human Osteoblasts’ Response to Biomaterials for Subchondral Bone Regeneration in Standard and Aggressive Environments

Pagani, Stefania (author), Salerno, Manuela (author), Filardo, Giuseppe (author), Locs, Janis (author), van Osch, G.J.V.M. (author), Vecstaudza, Jana (author), Dolcini, Laura (author), Borsari, Veronica (author), Fini, Milena (author), Giavaresi, Gianluca (author), Columbaro, Marta (author)

Osteochondral lesions, when not properly treated, may evolve into osteoarthritis (OA), especially in the elderly population, where altered joint function and quality are usual. To date, a collagen/collagen–magnesium–hydroxyapatite (Col/Col-Mg-HAp) scaffold (OC) has demonstrated good clinical results, although suboptimal subchondral bone...

journal article 2023

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White matter changes measured by multi-component MR Fingerprinting in multiple sclerosis

Nagtegaal, M.A. (author), Hermann, I. (author), Weingärtner, S.D. (author), Martinez-Heras, Eloy (author), Solana, Elisabeth (author), Llufriu, Sara (author), Gass, Achim (author), Poot, Dirk H.J. (author), van Osch, Matthias J.P. (author), Vos, F.M. (author), de Bresser, Jeroen (author)

T₂-hyperintense lesions are the key imaging marker of multiple sclerosis (MS). Previous studies have shown that the white matter surrounding such lesions is often also affected by MS. Our aim was to develop a new method to visualize and quantify the extent of white matter tissue changes in MS based on relaxometry properties. We...

journal article 2023

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A culture model to analyze the acute biomaterial-dependent reaction of human primary neutrophils in vitro

Wesdorp, Marinus A. (author), Schwab, Andrea (author), Bektas, Ezgi Irem (author), Narcisi, Roberto (author), Eglin, David (author), Stoddart, Martin J. (author), van Osch, G.J.V.M. (author), D'Este, Matteo (author)

Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials, the onset and resolution of chronic inflammation, and macrophage polarization. However, the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood. Here, we report an in vitro...

journal article 2023

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The role of cell-matrix interactions in connective tissue mechanics

Muntz, I.A.A. (author), Fenu, M. (author), van Osch, G.J.V.M. (author), Koenderink, G.H. (author)

Living tissue is able to withstand large stresses in everyday life, yet it also actively adapts to dynamic loads. This remarkable mechanical behaviour emerges from the interplay between living cells and their non-living extracellular environment. Here we review recent insights into the biophysical mechanisms involved in the reciprocal...

review 2022

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Intra-articular injection of triamcinolone acetonide sustains macrophage levels and aggravates osteophytosis during degenerative joint disease in mice

Ferrao Blanco, Mauricio N. (author), Bastiaansen Jenniskens, Yvonne M. (author), Kops, Nicole (author), Chavli, Athina (author), Narcisi, Roberto (author), Botter, Sander M. (author), Leenen, P.J.M. (author), van Osch, G.J.V.M. (author), Fahy, Niamh (author)

Background and purpose: Corticosteroids such as triamcinolone acetonide (TAA) are potent drugs administered intra-articularly as an anti-inflammatory therapy to relieve pain associated with osteoarthritis (OA). However, the ability of early TAA intervention to mitigate OA progression and modulate immune cell subsets remains unclear. Here, we...

journal article 2022

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Intra-articular Administration of Triamcinolone Acetonide in a Murine Cartilage Defect Model Reduces Inflammation but Inhibits Endogenous Cartilage Repair

Wesdorp, Marinus A. (author), Capar, Serdar (author), Bastiaansen-Jenniskens, Yvonne M. (author), Kops, Nicole (author), Creemers, Laura B. (author), Verhaar, Jan A.N. (author), van Osch, G.J.V.M. (author), Wei, Wu (author)

Background: Cartilage defects result in joint inflammation. The presence of proinflammatory factors has been described to negatively affect cartilage formation. Purpose: To evaluate the effect and timing of administration of triamcinolone acetonide (TAA), an anti-inflammatory drug, on cartilage repair using a mouse model. Study Design:...

journal article 2022