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Páez Watson, T.W. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)The understanding of microbial communities and the biological regulation of its members is crucial for implementation of novel technologies using microbial ecology. One poorly understood metabolic principle of microbial communities is resource allocation and biosynthesis. Resource allocation theory in polyphosphate accumulating organisms ...journal article 2023
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Pei, R. (author), Tarek-Bahgat, N. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Poly(3-hydroxybutyrate) (PHB) was accumulated in full-scale municipal waste activated sludge at pilot scale. After accumulation, the fate of the PHB-rich biomass was evaluated over two weeks as a function of initial pH (5.5, 7.0 and 10), and incubation temperature (25, 37 and 55 °C), with or without aeration. PHB became consumed under aerobic...journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...journal article 2023
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Wang, Xiangyang (author), Shi, Chen (author), Hao, Xiaodi (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Wet-chemical approach is widely applied for phosphate recovery from incinerated ash of waste activated sludge (WAS), along with metals removed/recovered. The high contents of both aluminum (Al) and iron (Fe) in WAS-incinerated ash should be suitable for producing coagulants with some waste anions like Cl<sup>−</sup> and SO<sub>4</sub><sup>2−<...journal article 2023
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Elahinik, A. (author), Li, L.H (author), Pabst, Martin (author), Abbas, B.A. (author), Xevgenos, Dimitris (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Enhanced biological phosphate removal and aerobic sludge granulation are commonly studied with fatty acids as substrate. Fermentative substrates such as glucose have received limited attention. In this work, glucose conversion by aerobic granular sludge and its impact on phosphate removal was studied. Long-term stable phosphate removal and...journal article 2023
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Kleikamp, H.B.C. (author), Grouzdev, Denis (author), Schaasberg, P. (author), van Valderen, R.D. (author), van der Zwaan, R. (author), van de Wijgaart, R. (author), Lin, Y. (author), Abbas, B.A. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)The tremendous progress in sequencing technologies has made DNA sequencing routine for microbiome studies. Additionally, advances in mass spectrometric techniques have extended conventional proteomics into the field of microbial ecology. However, systematic studies that provide a better understanding of the complementary nature of these ...journal article 2023
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Haaksman, V.A. (author), Schouteren, M. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)There is a growing interest to implement aerobic granular sludge (AGS) in existing conventional activated sludge (CAS) systems with a continuous flow-through configuration. The mode of anaerobic contact of raw sewage with the sludge is an important aspect in the adaptation of CAS systems to accommodate AGS. It remains unclear how the...journal article 2023
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Corbera Rubio, F. (author), Laureni, M. (author), Koudijs, N. (author), Müller, S. (author), van Alen, Theo (author), Schoonenberg, Frank (author), Lücker, Sebastian (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author)Rapid sand filters (RSF) are an established and widely applied technology for groundwater treatment. Yet, the underlying interwoven biological and physical-chemical reactions controlling the sequential removal of iron, ammonia and manganese remain poorly understood. To resolve the contribution and interactions between the individual reactions...journal article 2023
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Bahgat, T.M.S.M. (author), Wilfert, P.K. (author), Korving, Leon (author), van Loosdrecht, Mark C.M. (author)The study evaluated the combined phosphorus, nitrogen, methane, and extracellular polymeric substances (EPS) recovery from aerobic granular sludge (AGS) wastewater treatment plants. About 30% of sludge organics are recovered as EPS and 25–30% as methane (≈260 ml methane/g VS) by integrating alkaline anaerobic digestion (AD). It was shown that...journal article 2023
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van Loosdrecht, Mark C.M. (author), Hao, Xiaodi (author), Wu, Yuanyuan (author)摘要： 好氧颗粒污泥技术具有占地面积小、能耗低、剩余污泥量少、抗冲击性强等优点，是<br/>一种可持续的污水处理技术。以北京某污水处理厂实际生活污水作为基质，在中试规模（有效容积<br/>为2.5 m3）反应器中进行好氧颗粒污泥培养，研究了颗粒污泥在低温条件下的稳定性。中试自夏季<br/>开始运行到次年春季结束，共计运行260 d。结果表明，采用我国实际低碳源市政污水可以培养出好<br/>氧颗粒污泥，污泥颗粒化程度可达94%，SVI5/SVI30为1.1 ~ 1.3。冬季低温会造成颗粒污泥的枝状结<br/>构增多、丝状菌生长，进而引起颗粒化比例略有下降，为80%~91%，污泥平均粒径降低，SVI5/SVI30升<br/>高至1.5 ~ 1.8。在冬季低温条件下，通过优化调控进水流量...journal article 2022
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Kulshreshtha, Y. (author), Vardon, P.J. (author), Meesters, G.M.H. (author), van Loosdrecht, Mark C.M. (author), Mota, Nelson (author), Jonkers, H.M. (author)The water-resistance of cow-dung has made it a widely used stabiliser in traditional earthen structures in several Asian and African countries. Multiple studies have shown an improvement in water-resistance with the addition of cow-dung, but none provides insight into this behaviour. The present study investigates the water-resistance behaviour...journal article 2022
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Calderon Franco, D. (author), Sarelse, R.G. (author), Christou, S. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)In the One Health context, wastewater treatment plants (WWTPs) are central to safeguarding water resources. Nonetheless, many questions remain about their effectiveness in preventing antimicrobial resistance (AMR) dissemination. Most surveillance studies monitor the levels and removal of selected antibiotic resistance genes (ARGs) and mobile...journal article 2022
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van den Berg, L. (author), Toja Ortega, S. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Aerobic granular sludge (AGS) is an advanced biofilm-based technology for wastewater treatment. Diffusion of substrates into the granules is a key aspect of this technology. Domestic wastewater contains soluble organic substrates of different sizes that could potentially diffuse into the granules. In this study, the relation between the...journal article 2022
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Le, Quan H. (author), Verheijen, Peter J.T. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)This study deals with the application of data reconciliation to wastewater treatment processes which are subject to dynamic conditions and therefore do not reach a steady-state behaviour sensu stricto. The SHARON partial nitritation process, which is operated cyclically with alternating aerated and anoxic periods, is studied as an example....journal article 2022
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Cerruti, M. (author), Kim, J.H. (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Purple bacteria (PPB), anoxygenic photoorganoheterotrophic organisms with a hyper-versatile metabolism and high biomass yields over substrate, are promising candidates for the recovery of nutrient resources from wastewater. Infrared light is a pivotal parameter to control and design PPB-based resource recovery. However, the effects of light...journal article 2022
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Elahinik, A. (author), Haarsma, M.N. (author), Abbas, B.A. (author), Pabst, Martin (author), Xevgenos, Dimitris (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Glycerol is abundantly present in wastewater from industries such as biodiesel production facilities. Glycerol is also a potential carbon source for microbes that are involved in wastewater nutrient removal processes. The conversion of glycerol in biological phosphorus removal of aerobic granular sludge processes has not been explored to date....journal article 2022
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Petrik, Leslie Felicia (author), Ngo, Huu Hao (author), Varjani, Sunita (author), Xevgenos, Dimitris (author), Osseweijer, P. (author), van Loosdrecht, Mark C.M. (author), Smol, Marzena (author), Yang, Xuejing (author), Mateo-Sagasta, Javier (author)Eighty percent of wastewater is left untreated or not reused, exacerbating the water quality challenge, especially in vulnerable communities. This Voices asks: how can we improve wastewater management and convert wastewater into a resource?journal article 2022
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Kulshreshtha, Y. (author), Vardon, P.J. (author), Du, Y. (author), Habert, G. (author), Vissac, A. (author), Morel, J.C. (author), van Loosdrecht, Mark C.M. (author), Mota, Nelson (author), Jonkers, H.M. (author)Earthen construction is re-gaining popularity as an ecological and economical alternative to contemporary building materials. While building with earth offers several benefits, its performance due to water ingress is a concern for its widespread application. This limitation is often solved by adding chemical stabilisers such as Portland cement...journal article 2022
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Soler Jofra, A. (author), Schmidtchen, Lisbeth (author), Olmo, Lluc (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)Hydroxylamine is a nitrogen intermediate of ammonium oxidizing bacteria (AOB) that can transiently accumulate during nitrification. The impact of hydroxylamine on aerobic ammonium oxidations is still obscure. In the present study the short and long term impact of hydroxylamine on partial nitritation granular sludge was investigated. Dissolved...journal article 2022
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Wijdeveld, W. K. (author), Prot, T.J.F. (author), Sudintas, G. (author), Kuntke, P. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)Phosphorus (P) is an essential resource for food production and chemical industry. Phosphorus use has to become more sustainable and should include phosphorus recycling from secondary sources. About 20% of P ends up in sewage sludge, making this a substantial secondary P source. There is currently a technological gap to recover P from sludge...journal article 2022