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Cueto Rojas, H.F. (author), Maleki Seifar, R. (author), ten Pierick, A. (author), Heijnen, J.J. (author), Wahl, S.A. (author)Ammonium (NH4+) is the most common N-source for yeast fermentations, and N-limitation is frequently applied to reduce growth and increase product yields. While there is significant molecular knowledge on NH4 + transport and assimilation, there have been few attempts to measure the in vivo concentration of this metabolite. In this article, we...journal article 2016
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Cueto Rojas, H.F. (author), Maleki Seifar, R. (author), ten Pierick, A. (author), van Helmond, W. (author), Pieterse, M.M. (author), Heijnen, J.J. (author), Wahl, S.A. (author)Ammonium is the most common N source for yeast fermentations. Although its transport and assimilation mechanisms are well documented, there have been only a few attempts to measure the in vivo intracellular concentration of ammonium and assess its impact on gene expression. Using an isotope dilution mass spectrometry (IDMS)-based method, we...journal article 2016