Accurate Measurement of the in vivo  Ammonium Concentration in Saccharomyces cerevisiae

Ammonium (NH4+) is the most common N-source for yeast fermentations, and N-limitation is frequently applied to reduce growth and increase product yields. While there is significant molecular knowledge on NH4 + transport and assimilation, there have been few attempts to measure the in vivo concentration of this metabolite. In this article, we...