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Boender, L.G.M. (author), Van Maris, A.J.A. (author), De Hulster, E.A.F. (author), Almering, M.J.H. (author), Van der Klei, I.J. (author), Veenhuis, M. (author), De Winde, J.H. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)Extremely low specific growth rates (below 0.01 h?1) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-zero specific growth rates. While quiescence is...journal article 2011
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Van Zutphen, T. (author), Baerends, R.J.S. (author), Susanna, K.A. (author), De Jong, A. (author), Kuipers, O.P. (author), Veenhuis, M. (author), Van der Klei, I.J. (author)Background: Methylotrophic yeast species (e.g. Hansenula polymorpha, Pichia pastoris) can grow on methanol as sole source of carbon and energy. These organisms are important cell factories for the production of recombinant proteins, but are also used in fundamental research as model organisms to study peroxisome biology. During exponential...journal article 2010
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Gidijala, L. (author), Kiel, J.A.K.W. (author), Douma, R.D. (author), Seifar, R.M. (author), Van Gulik, W.M. (author), Bovenberg, R.A.L. (author), Veenhuis, M. (author), Van Klei, I.J. (author)This study aimed at developing an alternative host for the production of penicillin (PEN). As yet, the industrial production of this β-lactam antibiotic is confined to the filamentous fungus Penicillium chrysogenum. As such, the yeast Hansenula polymorpha, a recognized producer of pharmaceuticals, represents an attractive alternative....journal article 2009
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Sagt, C.M.J. (author), Ten Haaft, P.J.T. (author), Minneboo, I.M. (author), Hartog, M.P. (author), Damveld, R.A. (author), Van der Laan, J.M. (author), Akeroyd, M. (author), Wenzel, T.J. (author), Luesken, F.A. (author), Veenhuis, M. (author), Van der Klei, I. (author), De Winde, J.H. (author)Background: Enzyme production in microbial cells has been limited to secreted enzymes or intracellular enzymes followed by expensive down stream processing. Extracellular enzymes consists mainly of hydrolases while intracellular enzymes exhibit a much broader diversity. If these intracellular enzymes could be secreted by the cell the potential...journal article 2009
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Gidijala, L. (author), Bovenberg, R.A.L. (author), Klaassen, P. (author), Van der Klei, I.J. (author), Veenhuis, M. (author), Kiel, J.A.K.W. (author)Background: ?-Lactams like penicillin and cephalosporin are among the oldes known antibiotics used against bacterial infections. Industrially, penicillin is produced by the filamentous fungus Penicillium chrysogenum. Our goal is to introduce the entire penicillin biosynthesis pathway into the methylotrophic yeast Hansenula polymorpha. Yeast...journal article 2008
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- Beudeker, R.F. (author), Veenhuis, M. (author), Kuenen, J.G. (author) journal article 1981