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Calderon Franco, D. (author), Corbera Rubio, F. (author), Cuesta Sanz, M. (author), Pieterse, Brent (author), de Ridder, David (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author), Laureni, M. (author), Weissbrodt, D.G. (author)
Drinking water treatment plants (DWTPs) are designed to remove physical, chemical, and biological contaminants. However, until recently, the role of DWTPs in minimizing the cycling of antibiotic resistance determinants has got limited attention. In particular, the risk of selecting antibiotic-resistant bacteria (ARB) is largely overlooked in...
journal article 2023
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Chen, L.M. (author), Keisham, Sunanda (author), Tateno, Hiroaki (author), van Ede, J.M. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)
Bacteria can synthesize a diverse array of glycans, being found attached to proteins and lipids or as loosely associated polysaccharides to the cells. The major challenge in glycan analysis in environmental samples lies in developing high-throughput and comprehensive characterization methodologies to elucidate the structure and monitor the...
journal article 2023
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Li, Zejiao (author), Wang, Jixiang (author), Liu, Jialin (author), Chen, Xingyu (author), Lei, Zhongfang (author), Yuan, Tian (author), Lee, Duu Jong (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)
Reducing CO<sub>2</sub> emission and energy consumption is crucial for the sustainable management of wastewater treatment plants (WWTPs). In this study, an algal-bacterial aerobic granular sludge (AGS) system was developed for efficient carbon (C) assimilation and nitrogen (N)/phosphorus (P) removal without the need for mechanical aeration....
journal article 2023
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Li, Ji (author), Hao, Xiaodi (author), Shen, Zhan (author), Wu, Yuanyuan (author), van Loosdrecht, Mark C.M. (author)
A high moisture content of waste activated sludge (WAS) associated with a low calorific value needs to be deeply dried towards self-supporting incineration. On the other hand, thermal energy with low temperature exchanged from treated effluent has great potential for drying sludge. Unfortunately, low-temperature drying of sludge seems to be...
journal article 2023
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Kim, Nam Kyeun (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)
In the present research, a bio-based flame retardant (FR) was prepared using a biopolymer derived from wastewater sludge to improve the fire performance of polypropylene (PP). Extracellular polymeric substances (EPS), which were extracted from wastewater aerobic granular sludge, were absorbed into cellulose-based fibres, such as flax and...
journal article 2023
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Ochs, Pascal (author), Martin, Ben (author), Germain-Cripps, Eve (author), Stephenson, Tom (author), van Loosdrecht, Mark C.M. (author), Soares, Ana (author)
Over the past twenty years, various commercial technologies have been deployed to remove ammonia (NH<sub>4</sub>–N) from anaerobic digestion (AD) liquors. In recent years many anaerobic digesters have been upgraded to include a pre-treatment, such as the thermal hydrolysis process (THP), to produce more biogas, increasing NH<sub>4</sub>–N...
journal article 2023
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Elahinik, A. (author), Li, L.H (author), Pabst, Martin (author), Abbas, B.A. (author), Xevgenos, Dimitris (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)
Enhanced biological phosphate removal and aerobic sludge granulation are commonly studied with fatty acids as substrate. Fermentative substrates such as glucose have received limited attention. In this work, glucose conversion by aerobic granular sludge and its impact on phosphate removal was studied. Long-term stable phosphate removal and...
journal article 2023
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Kleikamp, H.B.C. (author), Grouzdev, Denis (author), Schaasberg, P. (author), van Valderen, R.D. (author), van der Zwaan, R. (author), van de Wijgaart, R. (author), Lin, Y. (author), Abbas, B.A. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)
The tremendous progress in sequencing technologies has made DNA sequencing routine for microbiome studies. Additionally, advances in mass spectrometric techniques have extended conventional proteomics into the field of microbial ecology. However, systematic studies that provide a better understanding of the complementary nature of these ...
journal article 2023
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Haaksman, V.A. (author), Schouteren, M. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)
There is a growing interest to implement aerobic granular sludge (AGS) in existing conventional activated sludge (CAS) systems with a continuous flow-through configuration. The mode of anaerobic contact of raw sewage with the sludge is an important aspect in the adaptation of CAS systems to accommodate AGS. It remains unclear how the...
journal article 2023
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Corbera Rubio, F. (author), Laureni, M. (author), Koudijs, N. (author), Müller, S. (author), van Alen, Theo (author), Schoonenberg, Frank (author), Lücker, Sebastian (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author)
Rapid sand filters (RSF) are an established and widely applied technology for groundwater treatment. Yet, the underlying interwoven biological and physical-chemical reactions controlling the sequential removal of iron, ammonia and manganese remain poorly understood. To resolve the contribution and interactions between the individual reactions...
journal article 2023
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Bahgat, T.M.S.M. (author), Wilfert, P.K. (author), Korving, Leon (author), van Loosdrecht, Mark C.M. (author)
The study evaluated the combined phosphorus, nitrogen, methane, and extracellular polymeric substances (EPS) recovery from aerobic granular sludge (AGS) wastewater treatment plants. About 30% of sludge organics are recovered as EPS and 25–30% as methane (≈260 ml methane/g VS) by integrating alkaline anaerobic digestion (AD). It was shown that...
journal article 2023
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Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)
The transformation of environmental microorganisms by extracellular DNA is an overlooked mechanism of horizontal gene transfer and evolution. It initiates the acquisition of exogenous genes and propagates antimicrobial resistance alongside vertical and conjugative transfers. We combined mixed-culture biotechnology and Hi-C sequencing to...
journal article 2023
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Sorokin, Dimitry Y. (author), Tikhonova, Tamara V. (author), Koch, Hanna (author), van den Berg, E.M. (author), Hinderks, Renske S. (author), Pabst, Martin (author), Kuenen, J.G. (author), van Loosdrecht, Mark C.M. (author), Lücker, Sebastian (author)
Dissimilatory nitrate reduction to ammonia (DNRA) is a common biochemical process in the nitrogen cycle in natural and man-made habitats, but its significance in wastewater treatment plants is not well understood. Several ammonifying Trichlorobacter strains (former Geobacter) were previously enriched from activated sludge in nitrate-limited...
journal article 2023
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Ali, Muhammad (author), Singh, Yogesh (author), Fortunato, Luca (author), Rehman, Zahid Ur (author), Manjunath, Sarvajith (author), Vrouwenvelder, J.S. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Saikaly, Pascal E. (author)
This study describes a novel integration of aerobic granular sludge (AGS) with a gravity-driven membrane (GDM) system at a pilot scale with a treatment capacity of approximately 150 L per day to treat raw domestic wastewater. The treatment performance and energy consumption of the AGS-GDM system were compared to the neighboring full-scale...
journal article 2023
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Yin, Xiaole (author), Chen, Xi (author), Jiang, Xiao Tao (author), Yang, Ying (author), Li, Bing (author), Shum, Marcus Ho Hin (author), Schmitt, H. (author), Cha, Chang Jun (author), van Loosdrecht, Mark C.M. (author)
Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys in human and animal sectors under the "One-Health"framework. However, there are substantial challenges in comparing and synthesizing the results of multiple studies that employ different test methods and...
journal article 2023
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Moradi, N. (author), Vazquez, Carlos Lopez (author), Hernandez, Hector Garcia (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Rincón, Francisco Rubio (author)
Digestate is a rich source of nutrients that can be applied in agricultural fields as fertilizer or irrigation water. However, most of the research about application of digestate have focused on its agronomic properties and neglected the potential harm of the presence of contaminants of emerging concern (CECs). Aadvanced oxidation processes ...
journal article 2023
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Li, Xuan (author), Liu, Huan (author), Gao, Li (author), Sherchan, Samendra P. (author), Zhou, Ting (author), Khan, Stuart J. (author), van Loosdrecht, Mark C.M. (author), Wang, Qilin (author)
Although the coronavirus disease (COVID-19) emergency status is easing, the COVID-19 pandemic continues to affect healthcare systems globally. It is crucial to have a reliable and population-wide prediction tool for estimating COVID-19-induced hospital admissions. We evaluated the feasibility of using wastewater-based epidemiology (WBE) to...
journal article 2023
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Pei, R. (author), Vicente-Venegas, Gerard (author), Tomaszewska-Porada, Agnieszka (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)
Polyhydroxyalkanoates (PHAs) can be produced with municipal waste activated sludge from biological wastewater treatment processes. Methods of selective fluorescent staining with confocal laser scanning microscopy (CLSM) were developed and optimized to evaluate the distribution of PHA storage activity in this mixed culture activated sludge...
journal article 2023
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de Jong, S.I. (author), Sorokin, Dimitry Y. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author), McMillan, D.G.G. (author)
Proteomics has greatly advanced the understanding of the cellular biochemistry of microorganisms. The thermoalkaliphile Caldalkalibacillus thermarum TA2.A1 is an organism of interest for studies into how alkaliphiles adapt to their extreme lifestyles, as it can grow from pH 7.5 to pH 11. Within most classes of microbes, the membrane-bound...
journal article 2023
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Pronk, M. (author), van Dijk, E.J.H. (author), van Loosdrecht, Mark C.M. (author)
book chapter 2023
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