- document
- de Zoete, M.C. (author) doctoral thesis 1995
- document
-
De Lathouder, K.M. (author)Recently, biocatalysis has become an interesting alternative for conventional industrial processes. Enzyme reactions are highly selective and fast, and can be performed under ambient conditions. A drawback of the use of enzymes is their fragile nature. Stirring, high temperature, or solvents can cause deactivation. Also separation of the free...doctoral thesis 2007
- document
-
Hiseni, A. (author), Arends, I.W.C.E. (author), Otten, L.G. (author)Two carotenoid 1,2-hydratase (CrtC) genes from the photosynthetic bacteria Rubrivivax gelatinosus and Thiocapsa roseopersicina were cloned and expressed in Escherichia coli in an active form and purified by affinity chromatography. The biochemical properties of the recombinant enzymes and their substrate specificities were studied. The purified...journal article 2011
- document
-
Hollmann, F. (author), Arends, I.W.C.E. (author)Biocatalysis is propagating into practically every area of organic chemistry, amongst them radical polymerizations. A review of the recent developments of this dynamic and quickly evolving area of research is presented together with a critical evaluation of its potential to yield novel polymers and/or environmentally more benign synthetic...journal article 2012
- document
-
De Torres, M. (author), Dimroth, J. (author), Arends, I.W.C.E. (author), Keilitz, J. (author), Hollmann, F. (author)Rh(III)-TsDPEN, an immobilized analog of the well-known [Cp*Rh(bpy)(H2O)]2+ was evaluated as a heterogeneous, recyclable regeneration catalyst for reduced oxidoreductase cofactors [NAD(P)H]. Repeated use of this catalyst was established and the catalytic properties were initially investigated. Apparently, Rh(III)-TsDPEN is prone to severe...journal article 2012
- document
- Straathof, A.J.J. (author) journal article 2013
- document
-
Chen, B.S. (author), Otten, L.G. (author), Resch, V. (author), Muyzer, G. (author), Hanefeld, U. (author)Rhodococcus rhodochrous ATCC 17895 possesses an array of mono- and dioxygenases, as well as hydratases, which makes it an interesting organism for biocatalysis. R. rhodochrous is a Gram-positive aerobic bacterium with a rod-like morphology. Here we describe the features of this organism, together with the complete genome sequence and annotation....journal article 2013
- document
-
Chen, B.S. (author), Resch, V. (author), Otten, L.G. (author), Hanefeld, U. (author)The enantioselective Michael addition using water as both nucleophile and solvent has to date proved beyond the ability of synthetic chemists. Herein, the direct, enantioselective Michael addition of water in water to prepare important ?-hydroxy carbonyl compounds using whole cells of Rhodococcus strains is described. Good yields and excellent...journal article 2014
- document
-
Gargiulo, S. (author)Oxidation of alcohols is a reaction of major interest for organic chemistry. However, the most common chemical routes developed so far involve the use of toxic or hazardous reagents or catalysts that often lack good chemoselectivity. In this respect, alcohol dehydrogenases (ADHs) represent a very valuable biocatalytic alternative, as they can...doctoral thesis 2015
- document
-
Chen, B. (author)?-Hydroxy carbonyl compounds are an important class of compounds often found as a common structural motif in natural products. Although the molecules themselves look rather simple, their synthesis can be challenging. Water addition to conjugated C = C bonds opens up a straightforward route for the preparation of ?-hydroxy carbonyl compounds....doctoral thesis 2015
- document
-
Hiseni, A. (author), Otten, L.G. (author), Arends, I.W.C.E. (author)Carotenoid 1,2-hydratases (CrtC) catalyze the selective addition of water to an isolated carbon–carbon double bond. Although their involvement in the carotenoid biosynthetic pathway is well understood, little is known about the mechanism by which these hydratases transform carotenoids such as lycopene into the corresponding hydroxyl compounds....journal article 2015
- document
-
Dominguez de Maria, P (author), Hollmann, F. (author)Biocatalysis is generally regarded as a “green” technology. This statement is justified by the mild reaction conditions, the use of aqueous reaction media—with water as the paradigm of green solvents—, and the renewable nature of the biocatalysts. However, researchers making these statements frequently do not take into account the entire picture...journal article 2015
- document
-
Fernandez Fueyo, E. (author), Younes, S.H.H. (author), Van Rootselaar, Stefan (author), Aben, René W M (author), Renirie, R. (author), Wever, Ron (author), Holtmann, Dirk (author), Rutjes, Floris P J T (author), Hollmann, F. (author)A catalytic, enzyme-initiated (aza-) Achmatowicz reaction is presented. The involvement of a robust vanadium-dependent peroxidase from Curvularia inaequalis allows the simple use of H<sub>2</sub>O<sub>2</sub> and catalytic amounts of bromide.journal article 2016
- document
-
Holtmann, Dirk (author), Hollmann, F. (author)Monooxygenases are promising catalysts because they in principle enable the organic chemist to perform highly selective oxyfunctionalisation reactions that are otherwise difficult to achieve. For this, monooxygenases require reducing equivalents, to allow reductive activation of molecular oxygen at the enzymes' active sites. However, these...journal article 2016
- document
-
Younes, S.H.H. (author), Ni, Y. (author), Schmidt, S. (author), Kroutil, Wolfgang (author), Hollmann, F. (author)Alcohol dehydrogenases are well-established catalysts for various reduction reactions. However, the reduction of carboxylic acid derivatives has not yet been reported with these enzymes. In this contribution, we demonstrated that carboxylic acid thioesters could be readily reduced by a range of alcohol dehydrogenases, albeit at significantly...journal article 2017
- document
-
Dong, J. (author), Fernandez Fueyo, E. (author), Hollmann, F. (author), Paul, C.E. (author), Pesic, M. (author), Schmidt, S. (author), Wang, Yonghua (author), Younes, S.H.H. (author), Zhang, W. (author)Oxidation chemistry using enzymes is approaching maturity and practical applicability in organic synthesis. Oxidoreductases (enzymes catalysing redox reactions) enable chemists to perform highly selective and efficient transformations ranging from simple alcohol oxidations to stereoselective halogenations of non‐activated C−H bonds. For many of...journal article 2018
- document
-
Huijbers, M.M.E. (author), Zhang, W. (author), Tonin, F. (author), Hollmann, F. (author)The photoenzymatic decarboxylation of fatty acids to alkanes is proposed as an alternative approach for the synthesis of biodiesel. By using a recently discovered photodecarboxylase from Chlorella variabilis NC64A (CvFAP) we demonstrate the irreversible preparation of alkanes from fatty acids and triglycerides. Several fatty acids and their...journal article 2018
- document
-
Hollmann, F. (author), Kara, Selin (author), Opperman, Diederik J. (author), Wang, Yonghua (author)Cyclic esters and amides (lactones and lactams) are important active ingredients and polymer building blocks. In recent years, numerous biocatalytic methods for their preparation have been developed including enzymatic and chemoenzymatic Baeyer–Villiger oxidations, oxidative lactonisation of diols, and reductive lactonisation and...journal article 2018
- document
-
Höfler, G.T. (author), Fernandez Fueyo, E. (author), Pesic, M. (author), Younes, S.H.H. (author), Choi, Eun Gyu (author), Kim, Yong H. (author), Urlacher, Vlada B. (author), Arends, I.W.C.E. (author), Hollmann, F. (author)A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD<sup>+</sup> into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the...journal article 2018
- document
-
Bracco Garcia, M.P. (author), Torrelo Villa, G. (author), Noordam, Sander (author), de Jong, Glenn (author), Hanefeld, U. (author)The hydroxynitrile lyase from Prunus amygdalus was immobilized on Celite R-633. The immobilized enzyme could successfully be utilized in buffer saturated MTBE and excellent conversions of benzaldehyde to R-mandelonitrile were observed. No leaching occurred. To achieve high enantioselectivities, the suppression of the undesired background...journal article 2018