Searched for: department%3A%22BT%255C%257Eslsh%257EBiotechnology%22
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Sleenhoff, S. (author), Osseweijer, P. (author)
With a mandatory labeling scheme for GM food in Europe since 2004 measuring actual consumer choice in practice has become possible. Anticipating Europeans negative attitude toward GM food, the labeling was enforced to allow consumers to make an informed choice. We studied consumers actual purchase behavior of GM food products and compared this...
journal article 2013
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Ursem, W.N.J. (author), Van de Vorle-Houben, E.J.J. (author), De Haar, J.A.W. (author)
The invention provides a gas purification system, for instance for agricultural application comprising a corona discharge system, the corona discharge system comprising a counter electrode, a conductive strip with a longitudinal edge comprising tooth structures, wherein the tooth structures have tooth tops with shortest distances selected from...
patent 2013
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Kuijpers, N.G. (author), Solis-Escalante, D. (author), Bosman, L. (author), Van den Broek, M. (author), Pronk, J.T. (author), Daran, J.M. (author), Daran-Lapujade, P.A.S. (author)
Background: In vivo recombination of overlapping DNA fragments for assembly of large DNA constructs in the yeast Saccharomyces cerevisiae holds great potential for pathway engineering on a small laboratory scale as well as for automated high-throughput strain construction. However, the current in vivo assembly methods are not consistent with...
journal article 2013
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Van Spronsen, J. (author), Witkamp, G.J. (author)
The invention is directed to a process for the recovery or removal of one or more crystallizable compounds from an aqueous solution containing, apart from the said crystallizable compounds, one or more organic or inorganic scale- forming or scale-inducing materials having a lower solubility in water than the said crystallizable compounds, said...
patent 2013
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Gonzalez-Ramos, D. (author), Van den Broek, M. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M.G. (author)
Background n-Butanol and isobutanol produced from biomass-derived sugars are promising renewable transport fuels and solvents. Saccharomyces cerevisiae has been engineered for butanol production, but its high butanol sensitivity poses an upper limit to product titers that can be reached by further pathway engineering. A better understanding of...
journal article 2013
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Hagen, W.R. (author)
EPR spectroscopy employs a resonator operating at a single microwave frequency and phase-sensitive detection using modulation of the magnetic field. The X-band spectrometer is the general standard with a frequency in the 9–10 GHz range. Most (bio)molecular EPR spectra are determined by a combination of the frequency-dependent electronic Zeeman...
journal article 2013
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Jorda, J. (author), Suarez Mendez, C.A (author), Carnicer, M. (author), Ten Pierick, A. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author), Ferrer, P. (author), Albiol, J. (author), Wahl, S.A. (author)
Background Several studies have shown that the utilization of mixed carbon feeds instead of methanol as sole carbon source is beneficial for protein production with the methylotrophic yeast Pichia pastoris. In particular, growth under mixed feed conditions appears to alleviate the metabolic burden related to stress responses triggered by protein...
journal article 2013
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Van Vugt-Lussenburg, B.M.A. (author), Van der Weel, L. (author), Hagen, W.R. (author), Hagedoorn, P.L. (author)
Background: The highly homologous [4Fe-4S] containing fumarases FumA and FumB, sharing 90% amino acid sequence identity, from Escherichia coli are differentially regulated, which suggests a difference in their physiological function. The ratio of FumB over FumA expression levels increases by one to two orders of magnitude upon change from...
journal article 2013
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Ursem, W.N.J. (author), Marijnissen, J.C.M. (author), Van de Vorle-Houben, E.J.J. (author)
The invention provides a gas purification system (10) for agricultural application comprising an elongated gas channel (100) having a rectangular cross-section, a channel height (h1), and a longitudinal axis (1); a gas transport unit (200); and a corona discharge system (300), comprising a counter electrode (340) within the elongated gas channel...
patent 2013
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Tamis, J. (author), Kleerebezem, R. (author), Van Loosdrecht, M.C.M. (author)
The invention is directed to a method for producing an open phototrophic culture with improved storage compound production capability. In accordance with the invention, a starting culture is submitted to selective pressure, thus giving a competitive advantage to storage compound producing species, by subjecting said starting culture to a cycle...
patent 2013
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Kartal, B. (author), De Almeida, N.M. (author), Maalcke, W.J. (author), Op den Camp, H.J.M. (author), Jetten, M.S.M. (author), Keltjens, J.T. (author)
Anaerobic ammonium-oxidizing (anammox) bacteria primarily grow by the oxidation of ammonium coupled to nitrite reduction, using CO2 as the sole carbon source. Although they were neglected for a long time, anammox bacteria are encountered in an enormous species (micro)diversity in virtually any anoxic environment that contains fixed nitrogen. It...
journal article 2013
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Gori, F. (author), Tringe, S.G. (author), Folino, G. (author), Van Hijum, S.A.F.T. (author), Op den Camp, H.J.M. (author), Jetten, M.S.M. (author), Marchiori, E. (author)
Background Sequencing technologies have different biases, in single-genome sequencing and metagenomic sequencing; these can significantly affect ORFs recovery and the population distribution of a metagenome. In this paper we investigate how well different technologies represent information related to a considered organism of interest in a...
journal article 2013
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Verhaert, P.D.E.M. (author)
journal article 2013
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Vrouwenvelder, J.S. (author), Prest, E.I.E.D. (author), Kuhl, M. (author), Van Loosdrecht, M.C.M. (author), Staal, M. (author)
journal article 2012
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Dreszer, C. (author), Vrouwenvelder, J.S. (author), Paulitsch-Fuchs, A.H. (author), Zwijnenburg, A. (author), Kruithof, J.C. (author), Flemming, H.C. (author)
journal article 2012
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Daran, J.M. (author), Pronk, J.T. (author)
In this feature, leading researchers in the field of environmental microbiology speculate on the technical and conceptual developments that will drive innovative research and open new vistas over the next few years. For the better part of four decades, genetic engineering has relied on a universal toolbox containing three indispensable...
journal article 2012
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Solis-Escalante, D. (author), Kuijpers, N.G.A. (author), Bongaerts, N. (author), Bolat, I. (author), Bosman, L. (author), Pronk, J.T. (author), Daran, J.M. (author), Daran-Lapujade, P.A.S. (author)
Despite the large collection of selectable marker genes available for Saccharomyces cerevisiae, marker availability can still present a hurdle when dozens of genetic manipulations are required. Recyclable markers, counterselectable cassettes that can be removed from the targeted genome after use, are therefore valuable assets in ambitious...
journal article 2012
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Koopman, F.W. (author), Beekwilder, J. (author), Crimi, B. (author), Van Houwelingen, A. (author), Hall, R.D. (author), Bosch, D. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
Background Flavonoids comprise a large family of secondary plant metabolic intermediates that exhibit a wide variety of antioxidant and human health-related properties. Plant production of flavonoids is limited by the low productivity and the complexity of the recovered flavonoids. Thus to overcome these limitations, metabolic engineering of...
journal article 2012
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Cruz, L.A.B. (author), Hebly, M. (author), Duong, G.H. (author), Wahl, S.A. (author), Pronk, J.T. (author), Heijnen, J.J. (author), Daran-Lapujade, P. (author), Van Gulik, W.M. (author)
Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in Saccharomyces cerevisiae during temperature changes....
journal article 2012
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Zhao, Z. (author), Ten Pierick, A. (author), De Jonge, L. (author), Heijnen, J.J. (author), Wahl, S.A. (author)
Background Penicillium chrysogenum, the main production strain for penicillin-G, has a high content of intracellular carbohydrates, especially reduced sugars such as mannitol, arabitol, erythritol, as well as trehalose and glycogen. In previous steady state 13C wash-in experiments a delay of labeling enrichments in glycolytic intermediates was...
journal article 2012
Searched for: department%3A%22BT%255C%257Eslsh%257EBiotechnology%22
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